A method for obtaining distant hybrids of sesame wild species and cultivated species by using immature embryo rescue technology

A technology of young embryo rescue and distant hybridization, applied in the fields of botanical equipment and methods, horticultural methods, applications, etc., can solve the problems of ineffective hybridization, abortion of hybrid embryos, and inability to obtain hybrid plants, and achieve good disease resistance and resistance. Inverse traits, improved germplasm, effects of overcoming incompatibility

Inactive Publication Date: 2017-03-08
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a kind of method that utilizes immature embryo rescue technology to obtain the distant hybridization progeny of sesame wild species and cultivated species, solves the problem of wild species sesame (S.radiatum, 2n=64) and cultivated species sesame (2n=26) The hybrids that existed during the distant hybridization were infertile, the hybrid embryos were aborted, and the hybrid F could not be obtained 1 Plants and other issues

Method used

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  • A method for obtaining distant hybrids of sesame wild species and cultivated species by using immature embryo rescue technology
  • A method for obtaining distant hybrids of sesame wild species and cultivated species by using immature embryo rescue technology
  • A method for obtaining distant hybrids of sesame wild species and cultivated species by using immature embryo rescue technology

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Embodiment 1

[0040] A method for obtaining distant hybrid offspring of wild sesame and cultivated sesame by using immature embryo rescue technology, comprising the following steps: (1) parental material preparation: the female parent selects a comprehensive trait comparison of 40-50 days from sowing to flowering. A good cultivar "Zhongzhi 14"; the male parent uses the wild species "Yezhi 5" (S.radiatum, 2n=64) as material, and selects disease-free and plump seeds for planting;

[0041] (2) Distant hybridization: when both parents are in bloom, remove the white corolla and stamens of the female parent, and list them; use the male parent to pollinate, record the number of hybrid flowers, and the number of pollinated capsules;

[0042] (3) Stripping of ovules: respectively select young capsules at the developmental stage of 12d to 18d after cross-pollination, rinse the surface with clear water, disinfect the surface of the young capsules with 75% alcohol for 30 seconds under the ultra-clean wo...

Embodiment 2

[0057]The method described in this example to use the immature embryo rescue technology to obtain the distant hybrid offspring of wild sesame and cultivated sesame is basically the same as that of Example 1, the difference is that: (1) the immature embryo induction medium is: based on MS Add 0.5mg / L gibberellin, 1mg / L 6-benzylaminoadenine, 30g / L sucrose and 8g / L agar in the medium, and the pH value of the medium is 5.8; Add 0.6mg / L IBA, 0.8mg / L AgNO3, 30g / L sucrose and 8g / L agar in the medium, and the pH value of the medium is 5.8; The culture conditions are: temperature 28~30°C, photoperiod 8h / d, light intensity 2000lux; (3) the rooting medium is: add 0.5mg / LNAA, 30g / L sucrose and 8g / L agar, the pH value of the medium is 5.8; the proliferation medium is: add, 3mg / L6-BA, 2mg / L IAA, 30g / L sucrose and 8g / L agar in the MS basic medium, the medium The pH value is 5.8.

[0058] The hybrid F obtained by crossing this embodiment 1 Plants, using molecular identification and cytolo...

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Abstract

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame wild species and cultivar distant hybrid progeny through adopting an immature embryo saving technology. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and sesame wild species (2n=64) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts the immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for obtaining distant hybrid offspring of sesame wild species and cultivated species by using immature embryo rescue technology. Background technique [0002] Sesamum (Sesamum indicumL, 2n=26) belongs to the genus Linaceae, and is an important high-quality oil crop and an advantageous agricultural product for export in my country. However, due to the long-term evolution and artificial selection of cultivated species, many good genes that originally existed in wild species have been eliminated, resulting in narrower and narrower genetic bases and poorer genetic diversity among hybrid parents. The existing sesame cultivars have poor resistance to disease and waterlogging, are easily affected by environmental conditions, and have poor yield stability. A large area is dead, and the yield and quality are greatly reduced, which seriously affects the developme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H1/02
Inventor 杨敏敏赵应忠刘红艳周婷郝国存周芳
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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