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Method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue

A technology of carnation eversummer and carnation, applied in the field of biotechnology breeding, can solve the problems of abortion of immature embryos, failure of hybridization, and inability to obtain hybrid plants, etc., and achieve the effect of optimizing breeding technology and enriching genetic basis

Pending Publication Date: 2022-07-12
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Problems solved by technology

[0006] The object of the present invention is to provide a method for obtaining interspecies hybrid offspring of Dianthus persicae and Carnation through embryo rescue, which solves the problems of infertility in hybridization, abortion of immature embryos, failure to obtain hybrid plants, etc. question

Method used

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  • Method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue
  • Method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue
  • Method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue

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Embodiment 1

[0032] A method for obtaining the offspring of the interspecific hybridization of Dianthus dianthus and Carnation through embryo rescue, comprising the following steps: (1) parent material preparation: the female parent selects Dianthus dianthus (2n=6X=90), and it is sown in autumn in 2020; the male parent selects cut flowers Type carnation "big peach red" (2n=2X=30) cutting in November 2020; parents meet in this flowering period in May 2021;

[0033] (2) Distant hybridization: After removing all the stamens of the female parent Dianthus dianthus when it blooms, it is isolated by bagging with sulfuric acid paper, and artificial pollination is carried out once a day on the 3rd to 5th day after flowering; on the parent stigma;

[0034] (3) Stripping of ovules: 7 to 10 days after cross-pollination, select capsules with enlarged calyx, rinse with clean water, disinfect with 75% alcohol for 30 seconds, and then use 0.1% HgCl 2 Sterilize for 3 to 4 minutes, then rinse with sterile ...

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Abstract

The invention belongs to the field of biotechnology breeding, and particularly relates to a method for obtaining interspecific filial generation of dianthus superbus and dianthus caryophyllus through embryo rescue, and the method specifically comprises the following steps: selecting dianthus superbus L as a female parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a female parent, selecting dianthus caryophyllus L as a male parent, selecting dianthus caryophyllus L as a female parent, and selecting dianthus caryophyllus L as a male parent; performing artificial pollination for interspecific hybridization; carrying out in-vitro culture on ovules; and culturing young embryos and transplanting seedlings. According to the method, the obstacle that hybrid plants cannot be obtained due to abortion of immature embryos during interspecific hybridization of the dianthus superbus and the dianthus caryophyllus is overcome by utilizing an embryo rescue technology, a large number of filial generations are obtained, and the hybrid plants inherit excellent characters of parents, are low and robust, are high in stress resistance, are large in flowers, bright in color and rich in flower fragrance and have extremely high ornamental value. According to the method, the interspecific cross breeding efficiency of the dianthus plants can be effectively improved, and the new variety breeding process of the dianthus flowers is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and particularly relates to a method for obtaining interspecific hybrid progeny of Dianthus perennials and Carnation through embryo rescue. Background technique [0002] Changxia Dianthus ( Dianthus plumarius L.), also known as the ground cover Dianthus, is a perennial herbaceous flower of the Dianthus family, native to Europe. Plant height 20 ~ 30cm, stems clustered, multi-branched, leaves linear-lanceolate, blue-gray. Flowers solitary or in clusters, pink or white, with long green period, three-season flowering, cold resistance, drought resistance, barren resistance, salinity resistance, less pests and diseases, strong adaptability to extensive management and other advantages. In gardens, it is often used for edging of flower beds or group planting in rock gardens, and can also be used for flower borders or road slope protection. [0003] Carnation ( Dianthus caryophyllus L.) Aliases...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008A01H4/002
Inventor 杨永恒张婷张永侠原海燕王银杰徐晓洋孙玉明刘清泉
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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