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Method for overcoming distant hybridization obstruction of poplar

A technology of poplar and hybrid breeding, which is applied in the field of overcoming barriers of distant hybridization of poplars, can solve problems such as abortion of young embryos, failure to obtain seedlings, etc., achieve good plumpness, overcome hybridization barriers, and achieve high seed-setting rates

Inactive Publication Date: 2013-06-19
辽宁省杨树研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic work in this area mainly includes, Xu Weiying (1988) used the glycoprotein treatment stigma technology of female parent pollen to make hybrids of Populus alba x Populus european, Populus tomentosa x Populus european, etc., but the immature embryos were still aborted
Zhang Jinfeng et al. (1999) carried out 21 hybrid experiments between poplar and black poplar.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, hybridization experiment of Chinese aspen × American black poplar

[0024] 1. Experimental materials

[0025] The female parent and male parent used in this embodiment are respectively:

[0026] Female parent: Chinese poplar (Populus davidiana) (Xing Yajuan. Research on the breeding of new strains of Chinese poplar and American poplar hybrids [D], Northeast Forestry University, 2005, the public can obtain from Populus Research Institute of Liaoning Province), for Populus (Populus) aspen poplar subpai poplar;

[0027] Male parent: American Black Poplar D189 (P.deltoides cv.'D189') (Dong Yan, Peng Rusheng et al. Research on Genetic Improvement of Liaoning Poplar, China Forestry By-products, 2005 (5), public available from Liaoning Province Poplar Research obtained), which is poplar of the genus Populus (Populus).

[0028] 2. Experimental method and operation steps

[0029] In this experiment, a total of 5 kinds of hybridization treatment methods were des...

Embodiment 2

[0051] Embodiment 2, hybridization experiment of Chinese aspen x American black poplar

[0052] 1. Experimental materials

[0053] The female parent and male parent used in this embodiment are respectively:

[0054] Female parent: Chinese poplar (Populus davidiana) (Xing Yajuan. Research on the breeding of new strains of Chinese poplar and American poplar hybrids [D], Northeast Forestry University, 2005, the public can obtain from Populus Research Institute of Liaoning Province), for Populus (Populus) aspen poplar subpai poplar;

[0055] Male parent: American Black Poplar D189 (P.deltoides cv.'D189') (Dong Yan, Peng Rusheng et al. Research on Genetic Improvement of Liaoning Poplar, China Forestry By-products, 2005 (5), public available from Liaoning Province Poplar Research obtained), which is poplar of the genus Populus (Populus).

[0056] 2. Experimental method and operation steps

[0057] In this experiment, a total of 5 kinds of hybridization treatment methods were des...

Embodiment 3

[0079] Embodiment 3, hybridization experiment of Chinese aspen × American black poplar

[0080] 1. Experimental materials

[0081] The female parent and male parent used in this embodiment are respectively:

[0082]Female parent: Chinese poplar (Populus davidiana) (Xing Yajuan. Research on the breeding of new strains of Chinese poplar and American poplar hybrids [D], Northeast Forestry University, 2005, the public can obtain from Populus Research Institute of Liaoning Province), for Populus (Populus) aspen poplar subpai poplar;

[0083] Male parent: American Black Poplar D189 (P.deltoides cv.'D189') (Dong Yan, Peng Rusheng et al. Research on Genetic Improvement of Liaoning Poplar, China Forestry By-products, 2005 (5), public available from Liaoning Province Poplar Research obtained), which is poplar of the genus Populus (Populus).

[0084] 2. Experimental method and operation steps

[0085] In this experiment, a total of 5 kinds of hybridization treatment methods were desi...

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Abstract

The invention discloses a method for overcoming distant hybridization obstruction of s poplar, in particular a method for processing a female parent pistil stigma in hybridization parents, which comprises the following steps of: (1) fumigating the pistil stigma of the female parent by volatile gas of normal hexane; and (2) coating aqueous extract solution of pollen of the female parent on the pistil stigma of the female parent processed through the step (1), wherein the female parent is a populus poplar, and the aqueous extract solution of the pollen is liquid which is obtained by extracting the pollen of the female parent through water and does not contain pollen grains. Due to the utilization of the method, incompatibility of hybridization between poplar sections or intro-sections can be effectively overcome, and particularly, the hybridization obstruction of poplar of white poplar section and populus aigeiros is effectively overcome. The experimental results show that the hybridization seeds obtained through the method has high maturing rate and good plumpness, the germination percentage of the seeds is more than 70 percent, and a new way is provided for obtaining an excellent hybridal clone for distant hybridization of the poplar.

Description

technical field [0001] The invention relates to a method for overcoming the obstacle of poplar distant hybridization. Background technique [0002] Populus tree species are divided into 5 factions according to their morphological characteristics, geographical distribution and hybrid affinity, namely: Leuce or Poplus, Aigeiros, Tacamahaca, Leucoides, and Populus euphratica. Pai (Turanga), poplar sect is divided into poplar sub-pai and aspen sub-pai, but intraspecific variation is abundant, and there are many natural and artificial hybrids between species, which makes the genetic relationship between different poplar sects or species different, and the genetic relationship is relatively high. Close poplars are prone to hybridization, while distantly related poplars have hybridization barriers. After Willing R.R and Pryor L.D. (1976) studied the interspecific hybridization of a variety of poplars, they believed that the compatibility between the green, black, and big-leaf popl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 王胜东彭儒胜纪纯阳赵鑫闻杨志岩刘毅赵继梅矫丽曼冯连荣蔄胜军刘巍张兴芬尹杰
Owner 辽宁省杨树研究所
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