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Fast breeding method of butterfly orchid and high-quality variety tissue culture propagation method

A technology of tissue culture and Phalaenopsis, which is applied in horticultural methods, botany equipment and methods, plant gene improvement, etc., can solve the problems of increasing the coefficient of variation of seedlings, failing to maintain the consistency of parent seedlings, separation, etc., to shorten the cycle , Realize the effect of rapid breeding and selection of fine varieties, high ornamental value

Inactive Publication Date: 2018-07-06
青州市亚泰农业科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the high-quality Phalaenopsis hybrids are propagated by aseptic sowing, the offspring will be separated, and the excellent characters of the mother and the consistency of the seedlings cannot be kept. Increase the coefficient of variation of seedlings

Method used

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  • Fast breeding method of butterfly orchid and high-quality variety tissue culture propagation method
  • Fast breeding method of butterfly orchid and high-quality variety tissue culture propagation method
  • Fast breeding method of butterfly orchid and high-quality variety tissue culture propagation method

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Embodiment 1

[0030] Embodiment one Phalaenopsis rapid breeding method

[0031] In the present embodiment, utilize the method for cross-breeding to carry out the rapid breeding of Phalaenopsis new variety, concrete steps are as follows:

[0032] 1) Hybrid pollination: use the selected Phalaenopsis species as parents, and use the mother plant that grows vigorously during flowering for pollination and hybridization, control the temperature at 27±3°C, and control the humidity at 60-80%; 120-150 days after pollination, the fruit Ripe seeds are used for sowing;

[0033] 2) Aseptic sowing: Soak the ripe fruit in alcohol with a mass concentration of 70-75% for 25-30 seconds, wash it with sterile water, and sterilize it with a mercuric chloride solution with a mass concentration of 0.10-0.12% for 10 seconds. ~15 minutes, then rinse with sterile water, cut the fruit on the ultra-clean workbench, spread the powdered seeds evenly in the germination medium, and germinate to form protocorms 30 to 38 da...

Embodiment 2

[0049] Embodiment two Phalaenopsis high-quality variety tissue culture propagation method

[0050] In the present embodiment, after the new variety Phalaenopsis is bred by the method described in Example 1, the experimental group 3 of the comparative experimental group 3 is selected, that is, the Phalaenopsis varieties bred by the male parent numbering YT-0231 and the female parent numbering YT-0215. A method of cultivating and propagating comprising the steps of:

[0051] 1) Disinfection treatment of explants: use the pedicels of the new species of Phalaenopsis selected as explants, use a sterilized blade to remove the outer bracts of the axillary buds at the nodes of the pedicels, and lighten them with alcohol with a mass concentration of 70-75%. After wiping the surface and cleaning it with sterile water, cut the pedicels into pedicel segments each containing one node, put them into a mercuric chloride solution with a mass concentration of 0.10-0.12% for 8-10 minutes and ke...

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Abstract

The invention discloses a fast breeding method of a butterfly orchid. Crossbreeding is carried out by utilizing an embryo culture technology, particularly, early-stage embryo culture technology is utilized to overcome the incompatibility of distant hybridization, and by rigidly controlling various links, including environmental conditions and breeding technology, of the whole breeding process, a cultured new variety of plants grow robustly and have excellent characters and a shortened breeding period, so that the fast breeding of a new variety and the selection of an excellent variety are realized. The invention further discloses a high-quality variety tissue culture propagation method of the butterfly orchid, for the selected high-quality new variety, an advantage that a pedicel internodesegment can be used for maintaining the characters of a stock plant in the tissue culturing process is utilized, pedicels of an excellent new variety, of which the seedling has stable characters, theblooming characters are excellent and the plant grows healthily, serve as an explant to perform tissue culture, and particularly preferential culture media are adopted in different phases, so that the butterfly orchid, which is cultured in a conventional condition, is fast in seedling emergence, good in stability, excellent in character and high in ornamental value.

Description

technical field [0001] The invention relates to the technical field of moth orchid cultivation and breeding, in particular to a rapid breeding method of moth orchid and a tissue culture propagation method of high-quality varieties. Background technique [0002] Phalaenopsis (Phalaenopsis) is a perennial herbaceous flower belonging to the Orchidaceae Phalaenopsis. It is native to tropical and subtropical regions. Its flower is shaped like a butterfly, with bright colors and long-lasting flowering. It has high ornamental value and economic value. One of the best-selling potted plants on the Internet. The earliest marketed potted varieties were selected from cut flower varieties with compact plant types, and now a large number of varieties suitable for potted plants have been bred. The breeding methods of Phalaenopsis mainly include: hybrid breeding, mutation breeding and biotechnology breeding, etc. Among them, hybrid breeding is to use intergeneric hybridization to introduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/008
Inventor 董春燕李天宇
Owner 青州市亚泰农业科技有限公司
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