Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving

A technology of immature embryo rescue and distant hybridization, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as hybridization is not fruitful, hybrid plants cannot be obtained, and hybrid embryos are aborted, so as to overcome incompatibility Effects of sex, improved germplasm, and enriched genetic base

Inactive Publication Date: 2015-12-02
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for obtaining the offspring of sesame distant hybridization by using immature embryos to save and solve the problem of distant hybridization between wild sesame and cultivated sesame, wild sesame and semi-wild sesame. The existing hybrids are not fertile, hybrid embryos are aborted, and hybrid F cannot be obtained 1 Plants and other issues

Method used

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  • Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving
  • Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving
  • Method for obtaining sesame distant hybrid progeny through adopting immature embryo saving

Examples

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Embodiment 1

[0037] A method for obtaining distant hybrid offspring of wild sesame and cultivated sesame by using immature embryo rescue technology, comprising the following steps: (1) parental material preparation: the female parent selects a comprehensive trait comparison of 40-50 days from sowing to flowering. For a good sesame cultivar 'Zhongzhi 14' (2n=26), the male parent is a wild sesame species 'Yezhi 3' (S. prostratum, 2n=58);

[0038] (2) Distant hybridization: when both parents are in bloom, remove the white corolla and stamens of the female parent, and list them; use the male parent to pollinate, record the number of hybrid flowers, and the number of pollinated capsules;

[0039] (3) Stripping of ovules: Select young capsules of different developmental stages such as after cross-pollination respectively, rinse their surfaces with clear water, disinfect the surface of young capsules with 75% alcohol for 30 seconds under an ultra-clean workbench, and then use 3% Disinfect with so...

Embodiment 2

[0052]The method described in this example to use the immature embryo rescue technology to obtain the distant hybrid offspring of wild sesame and cultivated sesame is basically the same as that of Example 1, the difference is that: (1) the immature embryo induction medium is: based on MS Add 0.5mg / LIAA, 1mg / L6-benzylaminoadenine, 30g / L sucrose and 8g / L agar in the medium, and the pH value of the medium is 5.8; the differentiation medium is: add 0.3mg / LIAA, 0.6mg / L6-benzylaminoadenine, 30g / L sucrose and 8g / L agar, the pH value of the medium is 5.8; (2) the immature embryo rescue in vitro induction culture and immature embryo differentiation culture The conditions are: temperature 28~30°C, photoperiod 8h / d, light intensity 2000lux; (3) The rooting medium is: add 0.5mg / LNAA, 0.3mg / LIBA, 30g / L sucrose and 8g / L agar, the pH value of culture medium is 5.8; Described proliferation medium is: add 3mg / L6-BA, 2mg / LIAA, 30g / L sucrose and 8g / L agar in MS basic medium , the pH of the med...

Embodiment 3

[0055] A method for obtaining distant hybrid offspring of wild sesame and cultivated sesame by using immature embryo rescue technology, comprising the following steps: (1) parental material preparation: the female parent selects a comprehensive trait comparison of 40-50 days from sowing to flowering. For good Congo wild sesame (S.schinzianum2n=64), the male parent is semi-wild sesame (S.malabaricum2n=26) 'Yezhi 2';

[0056] (2) Distant hybridization: when both parents are in bloom, remove the white corolla and stamens of the female parent, and list them; use the male parent to pollinate, record the number of hybrid flowers, and the number of pollinated capsules;

[0057] (3) Stripping of ovules: Select young capsules of different developmental stages such as after cross-pollination respectively, rinse their surfaces with clear water, disinfect the surface of young capsules with 75% alcohol for 30 seconds under an ultra-clean workbench, and then use 3% Disinfect with sodium hyp...

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Abstract

The invention belongs to the field of biotechnical breeding, and concretely relates to a method for obtaining sesame distant hybrid progeny through adopting immature embryo saving. The method concretely comprises the following steps: selecting a sesame cultivar (2n = 26) as a female parent and a sesame wild species S. prostratum (2n = 58) as a male parent, or selecting a sesame wild species S. schinzianum (2n = 64) as a female parent and a semi-wild species S. malabaricum (2n = 26) as a male parent; carrying out distant hybirdization on the male parent and the female parent, taking immature capsules 10-18d after hybridization pollination, and picking off ovules; inoculating the ovules into an immature embryo induction medium, and carrying out culture induction on germs to make the germs germinate in order to obtain immature embryos; inoculating the immature embryos into a differentiation medium, and culturing until the immature embryos develop into hybrid F1 seedlings; and carrying out propagation spreading cultivation on the hybrid F1 seedlings to obtain complete hybrid F1 plants. The method adopts an immature embryo saving technology to overcome the problem of easy immature embryo and no obtaining of the hybrid F1 plants in the distant hybirdization process of the sesame wild species and the sesame cultivar, and allows the disease and adverse stress resistance of the wild species to be transferred to the cultivar in order to effectively improve the germplasm of the sesame cultivar.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for obtaining the offspring of sesame distant hybridization by rescuing immature embryos. Background technique [0002] Sesame (Sesamumindicum L, 2n=26) belongs to the genus Linaceae, and is an important high-quality oil crop and characteristic agricultural product in my country. However, due to the long-term evolution and artificial selection of cultivated species, many good genes that originally existed in wild species have been eliminated, resulting in narrower and narrower genetic bases and poorer genetic diversity among hybrid parents. The existing sesame cultivars have poor resistance to disease and waterlogging, are easily affected by environmental conditions, and have poor yield stability. A large area is dead, and the yield and quality are greatly reduced, which seriously affects the development of sesame production. Wild sesame has formed ext...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02
Inventor 杨敏敏赵应忠刘红艳周婷郝国存周芳
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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