The invention relates to the technical field of hybrid breeding, in particular to a wheat hybridization method for increasing the setting rate. The method comprises the steps that when wheat grows to a flower bud differentiation period, a wheat growth promoter is sprayed to male parents and female parents; after female parent ears are completely headed, flag leaves are stripped off, one third of each spikelet is cut off, stamens are removed, a pistil growth promoter is sprayed, glassine bags are taken, the female parent ears are covered with the glassine bags, test tubes containing water are fixed in the glassine bags, male parent ears are cut out to be placed in the test tubes which are fixed in the glassine bags and contain water when the selected male parents hybridized with the female parents grow to a flowering period, the glassine bags are shaken in a hybridizing pollination period to complete natural pollination between the male parents and the female parents, and after pollination is completed, the glassine bags and the test tubes are taken down; when the pollinated female parents grow to a filling period, a filling period regulator is sprayed. According to the method, the pollination method is combined with nutrient supply of wheat in different periods, false hybridization is avoided, the hybrid breeding efficiency is improved, and the setting rate and the yield are increased.