Method for sterile seeding and rapid propagation of bougainvillea speetabilis

A technology of aseptic sowing and bougainvillea, applied in the field of plant tissue culture, can solve the problems of incomplete development of medium formulations for inducing sprouting and rooting, and achieve the effects of convenient germplasm exchange, high germination rate, and controllable culture conditions

Inactive Publication Date: 2016-11-09
海南省农业科学院热带园艺研究所
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Problems solved by technology

[0005] The object of the present invention is to provide a method for aseptic sowing and rapid propagation of bougainvillea, aiming at solving the collection and treatment of current seeds; the disinfection and sterilization method of seeds; the development of seed germination and bud-inducing medium formula; bud proliferation medium The development of the formula; the problem that the development of the medium formula for inducing sprouting and rooting is not perfect

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  • Method for sterile seeding and rapid propagation of bougainvillea speetabilis

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Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0025] The application principle of the present invention will be further described below in conjunction with the accompanying drawings.

[0026] Such as figure 1 Shown: bougainvillea aseptic sowing provided by the invention and rapid propagation method comprise the following steps:

[0027] S101: Induce germination by aseptic sowing with seeds: after sterilizing the seeds, insert them into medium MS+sucrose, and conduct bud induction culture at a suitable culture temperature, light time, and light intensity;

[0028] S102: Then use the buds to proliferate and multiply, and then induce rooting to breed seedlings: the germ...

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Abstract

The invention discloses a method for sterile seeding and rapid propagation of bougainvillea speetabilis. The method comprises the steps of conducting sterile seeding and induction germination on seeds, wherein the seeds are sterilized, then a shoot induction medium is inoculated with the sterilized seeds, and shoot induction culture is conducted at proper temperature with proper illumination time and illumination intensity; conducting seedling breeding through bud expanding propagation and rooting reinduction, wherein a sprout enrichment culture medium is inoculated with germinated buds, and bud propagation subculture is conducted at proper temperature with proper illumination time and illumination intensity; inoculating a rooting medium with the bud propagation subcultured bud seedlings, and conducting rooting breeding of seedlings at proper temperature with proper illumination time and illumination intensity. According to the method, seeds are used as breeding explant, seed treatment is easy, pollution rate is low, the germination rate of seeds reaches 100%, bud propagation rate reaches 5.22 times within 30 days, rooting rate is 95.6%, and seedling breeding is not limited by time or season so that whole-year production can be conducted.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for aseptic sowing and rapid propagation of bougainvillea. Background technique [0002] Bougainvillea glabra (Bougainvillea glabra) is a shrub of the genus Bougainvillea in the family Mirabilis. It has many flower colors, long flowering period, prosperous flower sense, and is resistant to barrenness, drought, and strong adaptability. At present, it has been introduced and cultivated almost all over the world, and is widely used in cities. Landscaping and ornamental potted plants are the national flower of the Republic of Zambia and the provincial flower of Hainan Province, Zhuhai City, Shenzhen City, Xiamen City, Liuzhou City, Sanya City, Haikou City and other cities. Bougainvillea is also a multi-purpose resource plant, and the beet pigment contained in it has been used to develop natural food pigments; pinitol isolated from its leaves and root...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 潘梅云勇符瑞侃杨珺戚华沙黄赛吕德任王景飞任军方
Owner 海南省农业科学院热带园艺研究所
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