Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid

A technology of gulonic acid and sorbose, which is applied in the field of promoting the transformation of L-sorbose into 2-keto-L-gulonic acid and the high-efficiency synthesis of 2-keto-L-gulonic acid to increase production and promote the growth of small bacteria , the effect of shortening the fermentation cycle

Active Publication Date: 2013-02-20
石药集团维生药业(石家庄)有限公司
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Problems solved by technology

[0006] The extracts of Astragalus and Gastrodia elata have certain effects on the growth of microorganisms, but so far there are

Method used

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  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid
  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid
  • Method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid

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Embodiment Construction

[0016] In order to better illustrate the present invention, the following examples are cited. However, the scope of the present invention is not limited thereto, and the scope of protection is described in the claims.

[0017] Examples 1-9, by using 0.00g / L, 0.50g / L, 0.75g / L, 1.00g / L, 1.25g / L, 1.50g / L, 1.75g / L, 2.00 g / L respectively in the fermentation medium g / L, 2.50 g / L addition amount M (g / L) add astragalus extract, and produce 2-keto by the following "method of promoting conversion of L-sorbose to 2-keto-L-gulonic acid" -L-gulonic acid, the steps are as follows:

[0018] 1. Seed cultivation

[0019] Weigh the ingredients of the seed medium according to the formula to prepare the seed medium (g / L): L-sorbose 20, yeast extract 3, beef extract 3, peptone 10, corn steep liquor 1.5, urea 1, KH 2 PO 4 1. MgSO 4 0.2, CaCO 3 1. Adjust the pH to 6.5-7.0, and sterilize at 121°C for 20 minutes;

[0020] Scrape a ring of mixed bacteria with a ring size and inoculate the law...

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Abstract

The invention discloses a method for promoting L-sorbose to convert to generate 2-keto-L-gulonic acid, belonging to the technical field of microbial fermentation. The method comprises two steps of seed cultivation and fermentative cultivation; according to the method, astragalus extract in an additive amount of 0.75-2.00g/L or gastrodia elata extract in an additive amount of 1.50-3.00g/L is added in a fermentation medium, or the astragalus extract and the astrodia elata extract in additive amounts of 1.00-1.50g/L and 1.50-2.00g/L are added in the fermentation medium at the same time, so as to promote the common ketogulonigenium vulgare (small bacterium) and huge bacillus (large bacterium) to coordinately grow to realize the efficient synthesis of the 2-keto-L-gulonic acid; and through coordinating the mutual functions of the two bacteria, the growth of the small bacterium is promoted, the output of the 2-keto-L-gulonic acid is improved and the fermentation period is shortened. According to the method, a 10L tank is used for the fermentation experiment and a predicted effect is obtained.

Description

technical field [0001] The invention relates to a method for promoting the transformation of L-sorbose into 2-keto-L-gulonic acid, in particular to a method for promoting common ketogenic radical by adding astragalus, gastrodia elata or extracts of astragalus and gastrodia elata into the fermentation medium. A method for synthesizing 2-keto-L-gulonic acid through the coordinated growth of Bacillus couronicum (small bacteria) and Bacillus megaterium (big bacteria). It belongs to the technical field of microbial fermentation. Background technique [0002] Vitamin C (Vitamin C, referred to as Vc), also known as L-Ascorbic acid (L-Ascorbic acid), is an essential vitamin for the human body. It has a wide range of physiological functions and plays an important role in the pharmaceutical and food industries. At present, the "two-step fermentation method" independently developed by China is widely used in my country. The first step of the "two-step fermentation method" is single-ba...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/60C12R1/01C12R1/11
Inventor 冯志军焦迎晖王清格马乐辉安卫红李会然王俊芬
Owner 石药集团维生药业(石家庄)有限公司
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