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Method for promoting growth and acid production of Ketogulonigenium vulgare

A technology of ketogulonic acid bacteria and lipoic acid is applied in the field of promoting the growth of ketogulonic acid bacteria and acid production, and can solve the problems of waste of nutrients and energy in fermentation medium, increased environmental pollution, increased process control, etc., and is beneficial to environmental protection. , the process is controllable, the effect of simplifying the production process

Inactive Publication Date: 2012-01-18
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Problems solved by technology

At present, VC production generally adopts the mixed bacteria fermentation method pioneered in my country. The small bacteria are acid-producing bacteria, but they grow slowly when cultured alone, are difficult to pass down, and have weak acid-producing ability. The mixed culture of large and small bacteria can promote the growth and development of small bacteria. Acid production, but the mixed fermentation of large and small bacteria increases the difficulty of process control, resulting in waste of nutrients and energy in the fermentation medium, increased environmental pollution, and increased production costs

Method used

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  • Method for promoting growth and acid production of Ketogulonigenium vulgare

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Experimental program
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Effect test

Embodiment 1

[0017] 1. Strains and plasmids Ketogulonigenium vulgare Y25 and Bacillus cereus L26 were purchased from Shiyao Weisheng Company.

[0018] 2. Medium HJY basal medium (1L): 15g sorbose, 3g yeast extract, 3g corn steep liquor, 15g peptone, 4g urea, 2g glucose, 0.2g MgSO 4 12H 2 O, 4g CaCO 3 , 5g KH 2 PO 4 , add dH 2 O to 1L, pH 6.8, autoclave at 110°C for 25min.

[0019] 3. The main reagent lipoic acid was purchased from SIGMA Company.

[0020] 4. The main solution lipoic acid solution: the concentration is 0.1mg / mL, dissolved in distilled water, sterilized by filtration with a 0.22μm microporous membrane, and stored at 4°C.

[0021] 5. The effect of lipoic acid on the growth of small bacteria

[0022] Pick a single colony of small bacteria and put it in 5mL HJY medium, cultivate it at 30°C and 200r / min for 72h to activate, and transfer it into fresh 5mL HJY basal medium according to the inoculum size of 1%. Add different amounts of lipoic acid solution to the final conce...

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Abstract

The invention provides a method for promoting growth and acid generation of Ketogulonigenium vulgare. By adding trophic factor lipoic acid into a Ketogulonigenium vulgare culture medium, the growth rate and the acid production capacity of the Ketogulonigenium vulgare can be improved, thereby the object that a single bacterium fermentation method realizes sugar and acid conversion is achieved, andthe fermentation process of vitamin C is simplified.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for promoting the growth and acid production of ketoclonic acid bacteria by adding exogenous nutritional factor lipoic acid. Background technique [0002] Vitamin C (vatamin C, VC) is a water-soluble vitamin, also known as L-ascorbic acid (L-ascorbic acid), which can participate in various hydroxylation reactions and redox reactions in the body, and is an important class of redox compounds in cell metabolism , plays an essential physiological role in the human body, and has important and extensive uses in many fields such as medicine, food, feed, and cosmetics. With the improvement of people's living standards and the increasing demand for VC, the research and improvement of its production process has been promoted, and the biological fermentation method is the development direction of industrial production of VC. [0003] my country's VC mixed bacteria...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12R1/01
Inventor 熊向华张惟材汪建华韩双侯伟
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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