Method for promoting growth and acid production of ketogulonigenium vulgare

Abstract

The invention discloses a method for promoting growth and acid production of ketogulonigenium vulgare. The method comprises the following steps: S1, seed culture: preparing a mixed bacterium of commonketogulonigenium vulgare and bacillus megaterium as a production strain; S2, preparing a culture medium solution; S3, preparing a seed culture solution; S4, preparing a seed culture medium; and S5, carrying out seed fermentation culture, maintaining the pH value in a fermentation tank at 6.2-7.2, and carrying out fermentation to generate 2-keto-L-gulonic acid; according to the method, lipoic acidis added into the seed culture medium, it is guaranteed that mixed bacteria of the common ketogulonigenium vulgare and bacillus megaterium can be directly subjected to single-bacterium fermentation through the small bacteria (ketogulonigenium vulgare), the fermentation process of 2-keto-L-gulonic acid is simplified, and in the seed fermentation culture process, electrolyte is added into the seedculture medium, so that the activity of seed fermentation is stimulated, the speed of seed fermentation is increased, and the purpose of efficiently producing 2-keto-L-gulonic acid is achieved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation engineering, in particular to a method for promoting the growth and acid production of ketoclonic acid bacteria. Background technique [0002] Vitamin C (vatamin C, VC) is a water-soluble vitamin, also known as L-ascorbic acid (L-ascorbic acid), which can participate in various hydroxylation reactions and redox reactions in the body, and is an important class of redox compounds in cell metabolism. It plays an essential physiological role in the human body, and has important and extensive uses in many fields such as medicine, food, feed, and cosmetics. With the improvement of people's living standards and the increasing demand for VC, the research and improvement of its production process has been promoted, and the biological fermentation method is the development direction of industrial production of VC. [0003] 2-keto-L-gulonic acid (2-keto-L-gulonicacid, referred to as 2-KLG) is...

AI Technical Summary

Problems solved by technology

[0004] Application No. CN201110235170.4 provides a method for promoting the growth and acid production of Ketogulonigenium vulgare; by adding the nutritional factor lipoic acid in the ketogulonigenium vulgare culture medium, the method of single-bacteria fermentation can be achieved The purpose of sugar-acid conversion simplifies the fermentation process of vitamin C; this method only clarifies that ketoglobulin itself cannot synthesize lipoic acid, and by adding lipoic acid, it is only a simplified process of promoting the growth of ketoglobulina, and does not Essentially increases the growth rate of ketoglobulina