Method for improving 2-keto-L-gulonic acid fermentation production strength

A keto-based cologne bacteria, production-intensive technology, applied in the field of microbial fermentation, can solve the problems of long cycle and high energy consumption

Inactive Publication Date: 2012-05-23
DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Compared with the foreign "Rayleigh method", the "two-step fermentation method" of vitamin C in China simplifies the process, reduces the cost and reduces the pollution. However, the second-step fermentation process still has the problems of long cycle and high energy consumption, which restrict production. Therefore, it is very necessary to improve the original fermentation production process and increase the production intensity of 2-KLG

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Connect 12ml of the cultured mixture of common ketogulonic bacteria and Bacillus megaterium to 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium hydrogen, 0.5% light calcium carbonate, 0.16% gelatin fermentation medium, the initial pH of the medium is 7.0, the culture temperature is 29°C, the volume of the shake flask is 750ml, the rotating speed of the shaker is 210rpm, and the final 2-KLG content of the fermentation broth is 79.91mg / ml , The fermentation cycle is 60 hours, which is 12 hours shorter than the control group without gelatin.

Embodiment 2

[0018] Connect 12ml of the cultured mixture of common ketogulonic bacteria and Bacillus megaterium to 80ml containing 8% sorbose, 0.5% corn steep liquor, 1.2% urea, 0.02% magnesium sulfate, 0.05% diphosphate Potassium hydrogen, 0.5% light calcium carbonate, 0.3% gelatin fermentation medium, the initial pH of the medium is 7.0, the culture temperature is 31°C, the volume of the shake flask is 750ml, the rotating speed of the shaker is 210rpm, and the final 2-KLG content of the fermentation broth is 79.36mg / ml , The fermentation period is 54 hours, which is 18 hours shorter than the control group without gelatin.

Embodiment 3

[0020] Connect 100 liters of the cultured mixture of common ketogenic gulonic bacteria and Bacillus megaterium to 350 liters containing 3% sorbose, 1% corn steep liquor, 0.2% urea, 0.03% magnesium sulfate, and 0.1% In the fermentation medium of potassium dihydrogen phosphate, 0.08% gelatin is added to the medium. The fermentation container adopts a 1000-liter automatic fermenter. The initial pH of the medium is 6.8, the culture temperature is 31℃, and the 25% sodium carbonate solution is used for 8 hours of culture. The pH of the fermentation broth was controlled to 7.0, and the sorbose solution with a concentration of 32% was added after 12 hours of cultivation. The final total sugar concentration of fermentation was 8.6%. During the fermentation process, the dissolved oxygen was controlled to 30-50%, and the 2-KLG content in the final fermentation broth was 84.77. mg / ml, the fermentation cycle is 32 hours, the acid production rate is 2.65mg / ml·h, which is 24.4% higher than tha...

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Abstract

The invention discloses a method for improving 2-keto-L-gulonic acid fermentation production strength. According to the method, a mixed strain comprising ketogulonigenium vulgare and bacillus megaterium is adopted as a production strain; L-sorbose is adopted as a substrate to carry out fermentation; 0.05-0.3% of gelatin is added to the fermentation culture media. According to the present invention, the process is adopted, such that the maximum generation rate of the 2-keto-L-gulonic acid and the maximum consumption rate of the sorbose in the fermentation liquid are respectively substantially improved, the fermentation period of the 2-keto-L-gulonic acid can be effectively shortened, and the vitamin C fermentation production strength can be improved.

Description

Technical field [0001] The invention relates to a preparation method of vitamin C, in particular to a method for improving the fermentation production intensity of 2-keto-L-gulonic acid, and belongs to the technical field of microbial fermentation. Background technique [0002] Vitamin C, or L-ascorbic acid, is an essential water-soluble vitamin for the human body and participates in various metabolic activities. At present, vitamin C is not only used as an important medical product to treat a variety of diseases, but also widely used in food, feed and cosmetics. It has developed from a single medicinal use to a multi-purpose product. 2-keto-L-gulonic acid (2-KLG) is an important precursor for the synthesis of vitamin C. At present, major domestic vitamin C manufacturers adopt the "two-step fermentation" production process invented by Yin Guanglin and others in the 1970s. The key step of this technology is the second fermentation process. L-sorbose is one It is biologically oxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/60C12R1/01C12R1/11
Inventor 孔太黄建民刘立明刘杰杜尔凤肖江锋陈克杰
Owner DSM JIANGSHAN PHARMACEUTICAL (JIANGSU) CO LTD
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