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Method for culturing chlorella by heterotrophism with sludge hydrolysate

A technology of chlorella and hydrolyzate, applied in the direction of microorganism-based methods, water/sludge/sewage treatment, sludge treatment, etc., can solve the problem of high cost of microalgae biodiesel, achieve resource utilization and save production cost effect

Inactive Publication Date: 2012-10-24
HARBIN INST OF TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of high cost of existing microalgae biodiesel, and to provide a method for cultivating chlorella heterotrophically using sludge hydrolyzate

Method used

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  • Method for culturing chlorella by heterotrophism with sludge hydrolysate
  • Method for culturing chlorella by heterotrophism with sludge hydrolysate
  • Method for culturing chlorella by heterotrophism with sludge hydrolysate

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specific Embodiment approach 1

[0015] Specific embodiment 1: A method for heterotrophic cultivation of chlorella using sludge hydrolysate in this embodiment is carried out according to the following steps: 1. Ultrasonic treatment of sludge: take the remaining sludge from the domestic sewage treatment plant and remove the The moisture content of the sludge is concentrated to a mass percentage of 98%~98.5%, and ultrasonic treatment is carried out for 8~10min under the conditions of a sound energy density of 5~10W / mL and a pulse ratio of 2:1; 2. Sludge hydrolysate Preparation of sludge: Anaerobic hydrolysis of the sludge after ultrasonic treatment in step 1 at a speed of 180r / min and a temperature of 35°C is carried out for 2~3d. During the hydrolysis process, the pH value is adjusted to 9.0 every 12h, and At a nitrogen flow rate of 0.3m 3 Under the condition of / h, the nitrogen is blown off every 12h to obtain the sludge hydrolysate; 3. Add trace elements and other nutrients to the sludge hydrolysate obtained i...

specific Embodiment approach 2

[0018] Specific embodiment two: this embodiment is different from specific embodiment one in that the pH value adjustment described in step two is carried out with 1 mol / L NaOH. Others are the same as the first embodiment.

specific Embodiment approach 3

[0019] Specific embodiment three: This embodiment is different from specific embodiments one to two in that the high-pressure steam sterilization operating conditions described in step three are: 121° C., 121 KPa, and sterilization for 30 minutes. Others are the same as the first to second specific embodiments.

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Abstract

The invention relates to a method for culturing chlorella by heterotrophism with sludge hydrolysate, relating to a method for culturing the chlorella through heterotrophism. The invention aims at solving the problem that the existing microalgae biodiesel is high in cost. The method disclosed by the invention comprises the following steps of: firstly, carrying out ultrasonic processing on excess sludge of a domestic sewage treatment plant; secondly, carrying out anaerobic hydrolysis on the sludge for 2-3 days under the conditions that rotating speed is 180r / min and temperature is 35 DEG C, regulating the pH value to 9.0, and carrying out nitrogen stripping every 12 hours, so as to obtain the sludge hydrolysate, adding trace elements except nitrogen and phosphorus and other nutrition elements, and carrying out high pressure steam sterilization, so as to obtain a culture solution; and thirdly, inoculating the chlorella in percentage by volume of 10-20%, and then culturing for 150-180 hours in a shaking culture tank, thus the culture of the chlorella is completed. By adopting the method disclosed by the invention, the cost for producing the chlorella can be saved, and low-cost raw materials are provided for commercial production of biodiesel. The method disclosed by the invention is applied to the biodiesel preparation field.

Description

Technical field [0001] The invention relates to a method for heterotrophically culturing chlorella. Background technique [0002] The exhaustion of fossil fuels is the focus of global attention, and the existing science and technology cannot solve the current energy demand problem. The large-scale application of crude oil has led to an increase in the price of fossil fuels (where the transportation system is the main consumer). At the same time, the increase in greenhouse gas emissions has caused environmental problems. The development of renewable energy has become a research hotspot in the field of energy and environment, such as solar energy, biomass energy, geothermal, water and wind energy, among which biomass energy. Because of its renewability, biodegradability, and non-toxicity, renewable energy has become a very potential energy source and has a large number of resource sources, such as agricultural and animal husbandry waste, municipal waste, and industrial waste , Te...

Claims

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Application Information

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IPC IPC(8): C12N1/12C02F11/00C02F11/10C02F11/06C02F11/02C12R1/89
CPCY02W10/33Y02W10/37Y02W10/40
Inventor 温沁雪陈志强李硼飞
Owner HARBIN INST OF TECH
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