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Saccharomyces cerevisiae strain enduring ferulic acid and application of saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae strains and ferulic acid, which is applied in the biological field, can solve the problems of Saccharomyces cerevisiae growth and strong inhibition of fermentation, and achieve the effects of stable genetics, good growth and fermentation performance, and enhanced tolerance

Active Publication Date: 2020-05-15
HEBEI NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to further solve the problem of the strong inhibitory effect of ferulic acid in the hydrolyzate of lignocellulosic biomass on the growth and fermentation of Saccharomyces cerevisiae

Method used

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  • Saccharomyces cerevisiae strain enduring ferulic acid and application of saccharomyces cerevisiae
  • Saccharomyces cerevisiae strain enduring ferulic acid and application of saccharomyces cerevisiae
  • Saccharomyces cerevisiae strain enduring ferulic acid and application of saccharomyces cerevisiae

Examples

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Embodiment 1

[0022] Example 1 Adaptive Evolution of Saccharomyces cerevisiae

[0023] Obtain the adaptive evolution method of ferulic acid-tolerant Saccharomyces cerevisiae strain PAT02, comprising the following steps:

[0024] (1) Configuration of synthetic medium: 20g of anhydrous glucose, 2g of potassium dihydrogen phosphate, 1g of magnesium sulfate, 1g of ammonium sulfate, and 10g of yeast extract powder per liter of medium; sterilize at 121°C for 20 minutes.

[0025] (2) Configuration of ferulic acid medium: Add ferulic acid to the above-mentioned sterilized synthetic medium so that the concentration of ferulic acid in the medium is (0.94-1.79g / L) to make ferulic acid culture base.

[0026] (3) Adaptive evolution of Saccharomyces cerevisiae to ferulic acid: Saccharomyces cerevisiae dry powder (Hubei Yichang Angel Yeast Co., Ltd.) was activated and cultured, and the activation culture conditions: 30°C in synthetic medium, 150 rpm Cultivate for 18 hours to obtain the original yeast st...

Embodiment 2

[0028] Example 2 Verification of tolerance of yeast strain PAT02 to ferulic acid

[0029] (1) Comparison of ferulic acid plate growth: adjust the cell density of yeast strain PAT02 activated for 18 hours and the original yeast strain to OD600 absorbance value 6.0, and then dilute the cell suspension in sequence (10 -1 、10 -2 、10 -3 ), and pipette 5 μL of the bacterial solution dropwise onto ferulic acid medium plates of different concentrations, and incubate at 30°C for 24 hours. Such as figure 1 As shown, the colony growth condition of the yeast strain PAT02 was significantly better than that of the original yeast strain.

[0030] (2) Verification of the genetic stability of ferulic acid tolerance: The yeast strain PAT02 activated for 18 hours was continuously transferred and cultured for 6 times in a synthetic medium without ferulic acid, and the culture conditions were: 30°C, 150 rpm divided into 12 hours each time, and then transfer it and the original yeast strain to...

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Abstract

The invention discloses a saccharomyces cerevisiae strain enduring ferulic acid. The saccharomyces cerevisiae strain is a strain which is obtained after adaptive evolution of saccharomyces cerevisiaewith a ferulic acid inhibitor culture medium and has remarkably improved endurance to ferulic acid, the strain is classified and named as Saccharomyces cerevisiae PAT02 and is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.18022. The saccharomyces cerevisiae strain is capable of enduring ferulic acid, and when the strain is appliedto a fermentation system with the ferulic acid, the growth performance and the ferulic acid degradation velocity of the strain are both higher than those of an original strain.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a ferulic acid-tolerant Saccharomyces cerevisiae strain and its application in a ferulic-acid-tolerant fermentation bacterial agent. Background technique [0002] Lignocellulosic biomass has become one of the most potential substitutes for fossil resources due to its rich resources, environmental friendliness and renewable characteristics. The transformation and utilization of biomass resources are of great significance to the country's energy security and development. Before enzymatic hydrolysis and conversion of lignocellulose into microbial fermentable sugars, lignocellulose needs to be pretreated with high temperature, high pressure and acid-base action to destroy its complex and dense structure. However, pretreatment will also produce by-products that have an inhibitory effect on microorganisms, including: phenols, furans and organic weak acids. Among them, compare...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12P7/06C12R1/865
CPCC12N1/18C12P7/06C12R2001/865C12N1/185Y02E50/10
Inventor 顾翰琦邵玲智刘晓光徐岩岩刘冉樊冰冰李洁
Owner HEBEI NORMAL UNIV FOR NATTIES
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