Method for preparing vanillin by virtue of immobilized biological membrane

A technology for vanillin and biofilm, which is applied in the field of immobilized biofilm to prepare vanillin, can solve the problems of limiting oxygen transfer and material exchange, unfavorable efficient production of vanillin, and large oxygen consumption, and achieves oxygen transfer and material exchange. The exchange is easy to maintain, reduces the risk of contamination, and the effect of efficient adsorption

Inactive Publication Date: 2014-08-13
甘肃省商业科技研究所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of vanillin fixed cell fermentation reports, only Bacillus fusiformis calcium alginate immobilized cells were used for vanillin conversion reports, but fermentation production requires a lot of oxygen consumption, the traditional embedding method It limits the oxygen transfer and material exchange in the vanillin fermentation process, which is not conducive to the efficient production of vanillin

Method used

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  • Method for preparing vanillin by virtue of immobilized biological membrane
  • Method for preparing vanillin by virtue of immobilized biological membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 A kind of method that immobilized biofilm prepares vanillin, comprises the following steps:

[0029] (1) At least one biofilm reactor filled with a fiber carrier is connected in series with the fermenter, and the volume of the biofilm reactor is 8% of the volume of the fermenter.

[0030] Wherein: the fiber carrier is composed of a fiber material and a support material, and the fiber material is wound into a barrel or spiral through the support material and fixed in the biofilm reactor. The fiber material refers to plant fibers; the supporting material refers to high-temperature-resistant plastics.

[0031] ⑵After adding the fermentation medium into the fermenter, together with the biofilm reactor, the pressure is 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C; the amount of fermentation medium added accounts for 50% of the volume of the fermentation tank.

[0032] Among them: the fermentation medium refers to mixing 1g ferulic acid, 1...

Embodiment 2

[0037] Example 2 A kind of method that immobilized biofilm prepares vanillin, comprises the following steps:

[0038] (1) At least one biofilm reactor filled with a fiber carrier is connected in series with the fermenter, and the volume of the biofilm reactor is 32% of the volume of the fermenter.

[0039] Wherein: the fiber carrier is composed of a fiber material and a support material, and the fiber material is wound into a barrel or spiral through the support material and fixed in the biofilm reactor. Fibrous material refers to animal fibers; supporting material refers to stainless steel materials.

[0040] ⑵After adding the fermentation medium into the fermenter, together with the biofilm reactor, the pressure is 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C; the amount of fermentation medium added accounts for 90% of the volume of the fermentation tank.

[0041] Among them: fermentation medium refers to mixing 0.2g ferulic acid, 5g peptone, 2g ye...

Embodiment 3

[0044] Example 3 A kind of method that immobilized biofilm prepares vanillin, comprises the following steps:

[0045] (1) At least one biofilm reactor filled with a fiber carrier is connected in series with the fermenter, and the volume of the biofilm reactor is 10% of the volume of the fermenter.

[0046] Wherein: the fiber carrier is composed of a fiber material and a support material, and the fiber material is wound into a barrel or spiral through the support material and fixed in the biofilm reactor. Fiber material refers to synthetic fibers; support material refers to aluminum material.

[0047] ⑵After adding the fermentation medium into the fermenter, together with the biofilm reactor, the pressure is 1.05Kg / cm 2 , Sterilize for 20 minutes at a temperature of 121°C; the amount of fermentation medium added accounts for 60% of the volume of the fermentation tank.

[0048] Among them: fermentation medium refers to mixing 1g ferulic acid, 15g peptone, 10g yeast extrac...

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Abstract

The invention relates to a method for preparing vanillin by virtue of an immobilized biological membrane. The method comprises the following steps: (1) connecting at least one biological membrane reactor filled with fiber carriers with a fermentation tank in series; (2) putting a fermentation medium in the fermentation tank, and then sterilizing the fermentation tank and the biological membrane reactor; (3) inoculating a slant culture of a ring of well-grown bacillus subtilis B7-S ((i)Bacillus subtilis( / i)B7-S) into a shake flask with the fermentation medium for shaking culture, so as to obtain a fermentation liquor; (4) allowing the fermentation liquor to circulate constantly between the biological membrane reactor and the fermentation tank by virtue of a pump, replacing with a fresh fermentation medium till the concentration of the vanillin is not increased, and continuing to ferment to produce the vanillin, wherein the time spent for each circulation of fermentation liquor is not more than 0.5 hour. The method can be used for effectively improving the production efficiency of the vanillin, has the advantages of producing the product with high concentration and being stable in production of each batch, and is suitable for industrial production of the vanillin.

Description

technical field [0001] The invention relates to the technical field of microorganisms and fermentation engineering, in particular to a method for preparing vanillin from an immobilized biofilm. Background technique [0002] Vanillin, also known as vanillin or vanillin or citronellal, the compound name is 4-hydroxy-3-methoxybenzaldehyde. It has a strong smell of vanilla orchid, and it is widely present in natural plants in the form of free and glucoside. Vanillin is widely used in the flavoring of food, beverages, tobacco and alcohol, washing cosmetics and medicines, and can also be used in the deodorization of industrial products such as plastics and rubber. It is also an important pharmaceutical raw material and industrial additive. In the food industry, vanillin has become one of the most popular edible spices for consumers in various countries. It has the reputation of "the king of food spices" and is widely used as a flavoring raw material for high-end foods such as fam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/24C12R1/125
Inventor 陈朋晏磊李素岳王宁波严晓娟梁宁
Owner 甘肃省商业科技研究所有限公司
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