A gene expression regulation method and regulation system based on type I-F CRISPR/Cas

A gene expression and target gene technology, applied in the field of molecular biology, to achieve high transcriptional activation efficiency and strong specificity
CN111979240BActive Publication Date: 2022-04-15SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN Ā· China
Patent Type
Patents(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2022-04-15

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Abstract

The invention discloses a gene expression regulation method and a gene expression regulation system based on Type Iā€‘F CRISPR / Cas. The present invention shows for the first time that the Cascade complex belonging to the Type I-F CRISPR / Cas system, such as the PaeCascade complex, can efficiently bind to the target site in mammalian cells; at the same time, the present invention conducts the Type I-F CRISPR / Cas system The mammalian expression system has been optimized so that it can efficiently recruit transcriptional activators or transcriptional repressors to target sites in mammalian cells, and successfully constructed a transcriptional activation system and a transcriptional repression system. The invention makes it possible to apply the Type I-F CRISPR / Cas system in the gene regulation of mammalian cells, and provides a necessary tool for gene transcription regulation based on the Type I-F CRISPR / Cas system.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology. More specifically, it relates to a type I-F CRISPRCas system-based gene expression regulation method, gene expression regulation system and application. Background technique

[0002] Gene expression regulation and gene editing are key technologies in the field of targeted gene therapy. Gene expression regulation is different from gene editing. Gene expression regulation refers to the regulation and control of gene expression in organisms, so that the process of gene expression in cells is in an orderly state in time and space, and a complex process that responds to changes in environmental conditions. The regulation of gene expression can be carried out at multiple levels, including gene level, transcription level, post-transcription level, translation level and post-translation level regulation. The CRISPR / Cas system is a reported high-efficiency gene-level manipulation method, which...

Claims

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