Recombinant strain for synthesizing hypoxanthine and construction method and application of recombinant strain

A technology of hypoxanthine and recombinant bacteria, applied in the field of genetic engineering, can solve the problems of difficult to achieve purine accumulation, long synthetic route, and difficult to meet the needs of large-scale biosynthesis of purine compounds
CN110656074AActive Publication Date: 2020-01-07QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Publication Date
2020-01-07

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Abstract

The invention discloses a recombinant strain for synthesizing hypoxanthine and a construction method and application of the recombinant strain, and belongs to the technical field of genetic engineering. According to the recombinant strain, escherichia coli is used as an original strain, a transcription repressor protein gene purR, a phosphogluconate anhydrase gene edd, an adenylosuccinic acid synthetase gene purA and an inosinic acid dehydrogenase gene guaB on an escherichia coli genome are respectively knocked out, and besides, D128A mutated PRPP synthetase gene prs and K326Q as well as a P410W double-mutated PRPP transamidase gene purF are subjected to overexpression. Besides, the invention further provides a preparation method of the recombinant strain and a method for producing the hypoxanthine through the recombinant strain. The efficient biosynthesis of the hypoxanthine in engineering escherichia coli is realized for the first time. The recombinant strain is suitable for production of the hypoxanthine through fermentation.
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Description

technical field

[0001] The invention relates to a recombinant bacterium for synthesizing hypoxanthine and its construction method and application, belonging to the technical field of genetic engineering. Background technique

[0002] Purine is a class of important life-active substances, which participate in the synthesis of genetic information substances in cells. The intermediate metabolites in the process of purine biosynthesis play an important role in the transfer of genetic material, signal transport and energy metabolism in the process of cell life. In addition, purine and its derivatives are also widely used in the field of medicine, such as the diagnosis and treatment of diseases and research fields such as life sciences, which has aroused people's strong interest in the synthesis of purine and its derivatives. At present, derivatives with purine as the backbone have been widely used in drug research, for example, purine compounds can induce the release of interfer...

Claims

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