The invention relates to an automatic sample injector bottle pressing assembly, which comprises a needle seat, a needle assembly, a bottle pressing guide rail, a tension spring, a position limiting frame and a bottle pressing block, wherein a sliding block is fixed on the needle seat, the needle assembly is fixed on the needle seat, the bottle pressing guide rail is provided with a sliding groove,the sliding groove and the sliding block form a sliding pair in a matched manner so as to make the bottle pressing guide rail slide relative to the needle seat in a direction parallel to the needle assembly, the upper end of the tension spring is fixed on the bottle pressing guide rail, the lower end of the tension spring is fixed on the needle seat, the position limiting frame is fixed on the bottle pressing guide rail and is used for detecting whether the bottle is deficient by matching with a bottle deficiency detection opticalcoupler, the bottle pressing block is fixed on the lower end ofthe bottle pressing guide rail and is provided with a through hole concentric with the needle assembly, and the needle assembly can penetrate through the through hole of the bottle pressing block. According to the present invention, the automatic sample injector bottle pressing assembly can detect the bottle deficiency, can prevent the automatic sample injector from idle walking, can reduce the failure probability of the puncture action of the automatic sample injector, can improve the reliability, can effectively balance the pressures inside and outside the sample injection bottle, and can ensure the accuracy of the sample injection volume.