The invention discloses an acoustic wave puncture auxiliary system and a method for applying the same. The acoustic wave puncture auxiliary system comprises a loudspeaker, a voltage-controlled oscillator, a display and recording module, a pressure signal amplifier, a pressure sensor, a piston tee, an infusion pump, an epidural puncture needle, a polyvinyl chloride extension tube and a polyethylene extension tube. The loudspeaker is connected with the display and recording module and the pressure signal amplifier by the voltage-controlled oscillator, the pressure signal amplifier is connected with the polyvinyl chloride extension tube and the polyethylene extension tube by the pressure sensor, the other end of the polyvinyl chloride extension tube is connected with the infusion pump, and the other end of the polyethylene extension tube is connected with the epidural puncture needle. The acoustic wave puncture auxiliary system and the method have the advantages that the position of the epidural puncture needle can be judged by the aid of acoustic waves and screen waveforms, accordingly, direct effects can be realized, an anesthetist can hold the puncture needle by the two hands, the stability and the controllability of puncture actions can be facilitated, the puncture success rate can be increased and nearly reaches 100%, and the acoustic wave puncture auxiliary system further can assist in judging the positions of indwelling catheters or can be used for clinically teaching new anesthetists.