New use of sodium hydrosulfide for promoting wheat seeds sprouting under heavy metal stress

A technology of wheat seeds and sodium hydrosulfide, applied in the field of known compounds, to achieve the effects of promoting normal growth, alleviating oxidative damage, and promoting stress resistance and germination

Inactive Publication Date: 2009-03-18
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In plants, about H 2 S as a signaling molecule to regulate plant physiological responses has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1, sodium hydrosulfide promotes Al 3+ Germination experiment of wheat seeds under poisoning

[0012] Weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) Add water to prepare a 0.6mmol / L aqueous solution. Wheat seeds were pretreated (soaked, the same below) in 0.6mmol / L sodium hydrosulfide (NaHS) aqueous solution at room temperature for 12 to 24 hours, then the seeds were fished out and sown in 30mmol / L Al 3+ Pollution (under this concentration condition, the germination rate of wheat seeds is 50% of the control group, called Al 3+ half inhibitory concentration) growth environment. The germination rate of seeds 48 hours after sowing increased by 40% compared with the water pretreatment control.

Embodiment 2

[0013] Embodiment 2, sodium hydrosulfide promotes Cr 3+ with Cr 6+ Germination experiment of wheat seeds under poisoning

[0014] Weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) add water to prepare 1.2mmol / L concentration aqueous solution. Wheat seeds were pretreated with 1.2mmol / L sodium hydrosulfide (NaHS) aqueous solution at room temperature for 12 to 24 hours, then the seeds were removed and sown on 4mmol / L Cr 3+ with Cr 6+ Pollution (under this concentration condition, the germination rate of wheat seeds is 50% of the control group, called Cr 3+ with Cr 6+ half inhibitory concentration) in the growth environment. The germination rate of seeds 48 hours after sowing increased by 38% compared with the water pretreatment control.

Embodiment 3

[0015] Embodiment 3, sodium hydrosulfide promotes Cd 2+ Germination experiment of wheat seeds under poisoning

[0016] Weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) add water to prepare 1.5mmol / L concentration aqueous solution. Wheat seeds were pretreated in 1.5mmol / L sodium hydrosulfide (NaHS) aqueous solution at room temperature for 12-24 hours, then the seeds were removed and sown on 7mmol / L Cd 2+ Contamination (under this concentration condition, the germination rate of wheat seed is 50% of the control group, called Cd 2+ half inhibitory concentration) in the growth environment. The germination rate of seeds 48 hours after sowing increased by 45% compared with the water pretreatment control.

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Abstract

The invention discloses a new application of sodium hydrosulfide for promoting germination of wheat seeds under the stress of heavy metals, namely, the application is that the sodium hydrosulfide is taken as a sprout-promoting agent of germination in the soil polluted by heavy metals of Al<3+>, Cd<2+>, Cr<3+>, Cr<6+>, Pb<2+>, Hg<2> and the like. The invention can effectively improve the germinability of the wheat seeds under the stress of heavy metals, and induces the seeds to germinate fast and regularly. Research proves that the sodium hydrosulfide of the hydrogen sulfide donor is adopted to preprocess the wheat seeds, which can remarkably induce the activity expression of hydrolytic enzyme of amylase, protease, lipase and the like, in the seeds, enhances the oxygenolysis of storage matter, relieves the oxidative damage caused by the stress of the heavy metals in the seeds and maintains the lower membrane lipid overoxidation level in the seeds and the seedlings, thus being beneficial to the normal growth of embryo and radicle. The invention solves the key problem of restoring the production of the wheat in the areas polluted by heavy metals, has simple operation; the needed reagent is single and is easy to be obtained.

Description

1. Technical field [0001] The present invention relates to a new application of a known compound, in particular to a new application of sodium hydrosulfide (NaHS) for improving germination of wheat seeds under heavy metal stress. 2. Background technology [0002] In recent years, the massive discharge of industrial wastes, the excessive development and utilization of mineral resources, and the extensive use of various pesticides and fertilizers in agricultural production have led to the gradual expansion of the land area polluted by heavy metals. Heavy metal pollution is an important aspect of soil pollution, which directly affects the production of crops, and countries all over the world attach great importance to it. The independent life of the next generation of plants usually begins with seed germination, which is restricted by external environmental factors, including light, temperature, water, oxygen, and soil factors. Wheat is an important food crop with a large sowi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/02A01P21/00A01G1/00A01G7/00B09C1/08
Inventor 张华焦浩窦伟姜绍通罗建平
Owner HEFEI UNIV OF TECH
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