94 results about "Milk fat globule" patented technology

Humanized antibody molecules (HAMs) are described having specificity for human milk fat globule and having an antigen binding site wherein at least one of the complementarity determining regions (CDRs) of the variable domains is derived from the mouse monoclonal antibody CTMO1 and the remaining immunoglobulin-derived parts of the HAM are derived from a human immunoglobulin. The HAMs may be chimeric humanized antibodies or CDR-grafted humanized antibodies and are preferably produced by recombinant DNA techniques. The HAMs are useful for in vivo diagnosis and therapy.

The present disclosure relates to a lipid source for nutritional compositions, comprising an enriched lipid fraction which comprises structured fat globules. The enriched lipid fraction provides fat globules having a desired size and fatty acid composition and may be stabilized by components such as phospholipids, cholesterol, milk-fat globule membrane protein and combinations thereof. Additionally, the disclosure relates to methods of supporting lipid digestion in a pediatric subject by providing a nutritional composition comprising an enriched lipid fraction having structured fat globules that are more accessible to lipases. The chemical composition, size and structure of the fat globules may improve digestion. The disclosed nutritional compositions may provide additive and or / synergistic beneficial health effects.

A method and composition for administering leptin to a subject. The invention includes suspending isolated native leptin-containing milk fat globules in a suitable medium for administering to a subject. The suspended milk fat globules may be administered orally as well as by intravenous, intramuscular, intraperitoneal, other enteral routes of administration, and other parenteral routes of administration. The invention includes a method for treating growth or maturational-related disorders in newborns as well as subjects having conditions that can be treated by the administration of leptin.

A nutritional composition is proposed comprising a probiotic and milk fat globule membrane (MFGM) wherein the MFGM and the probiotics mutually potentiate their respective effects and promote complementary beneficial protective mechanisms that reflect physiological synergy. The nutritional composition can be an infant formula, infant cereals, baby food or health care nutritional product. The synergistic effect can include promoting immune maturation and / or immune education and / or reducing inflammation and / or treating or preventing disease or infections.

The present invention provides recombinant peptides that specifically and selectively bind to the human milk fat globule (HMFG) antigen, BA46. In particular, the present invention provides recombinant variants of the Mc3 antibody, including humanized versions of Mc3. The variant Mc3 peptides are particularly useful for diagnostic, prognostic, and therapeutic applications in the field of breast cancer. The present invention also provides methods for the humanization of antibodies such as murine monoclonal antibodies. The novel humanization methods are applied to the production of humanized Mc3 antibodies and it is shown that these humanized antibodies retain the ability to engage in high affinity binding to their cognate antigen. Such humanization enables the use of these antibodies for immunodiagnostic and immunotherapeutic applications in humans.

A milk fat globule membrane-containing infant formula milk powder and a preparation method thereof belong to the technical field of preparation of infant formula milk powder. The milk fat globule membrane-containing infant formula milk powder is composed of essential nutrient components of skim milk powder, concentrated whey protein powder, lactose, demineralized whey powder, vegetable oil, vitamins, mineral substance and the like and meanwhile contains 0.01%-1.5% of milk fat globule membrane (MFGM) extract. By taking breast milk as reference, the nutrient components of the milk fat globule membrane-containing infant formula milk powder, especially the composition of fat globules, can be closer to those of the breast milk and accordingly can promote cerebral and neural development of newborns and improve the cognitive competence as well as anti-infection ability.

The invention discloses baby milk powder of which micro functional active components in a formula are comprehensively adjusted and a preparation method of the baby milk powder, and belongs to the field of foods. The baby milk powder of which the micro functional active components in the formula are comprehensively adjusted, provided by the invention, comprises the following components of skimmed milk powder, refined vegetable oil, desalinated whey powder, whey protein powder, lactose, OPO structure fat, galacto oligosaccharide, fructo-oligosaccharide, condensed lactalbumin whey albumen powder,compound minerals and compound vitamins, wherein the whey protein powder is the whey protein powder rich in milk fat globule membranes (MFGM) and the whey protein powder rich in casein glycomacropeptide(CGMP). The composition and the content of micro active components in the baby milk powder provided by the invention approach the levels of breast milk.

Provided is a preparation method and device of personalized nano ice cream. Liquid nitrogen and ice cream raw materials are injected into a material container installed in a rotating-revolving mixer in a jet mode, the container containing the liquid nitrogen and ice cream raw materials performs high-speed revolution and rotation in a revolution track through the rotating-revolving mixer. Therefore, revolution energy having powerful accelerated speed is produced, meanwhile rotation makes the materials in the container to produce convection, accordingly full stirring, dispersion and defoaming within short time are achieved, and uniformly distributed nano ice crystals are efficiently formed. High-speed revolution and rotation mixing make the milk fat globules in the ice cream raw materials smaller and more uniformly emulsified, and finally a stable nano ice crystal-milk fat globule-microbubble structure is formed. The material container utilizes a container adapter for placement, common ice cream can be placed in a machine for direct processing, and container cleaning and waste are completely avoided. The production process is simple and stable, no stabilizer nor emulgator is needed in the preparation process, and the made ice cream has a fine and smooth taste and strong milk fragrance and is more natural.

The invention provides infant formula milk powder rich in milk fat globule membrane protein and phospholipid. By adding alpha-lactalbumin, milk fat globule membrane and a physiological activator, suchas bifidobacterium animalis Bb-12, milk fat globule membrane lipids and protein, N-acetylneuraminic acid (sialic acid), SN-2 structure lipid, human milk oligosaccharides (HMOs) and human milk oligose(HMO). By simultaneously adjusting the using amount ratio of all the components, especially sphingomyelin, gangliosides, the milk fat globule membrane protein, the human milk oligosaccharides, the human milk oligose, adiponectin and lysozyme, and the proportion between mucin, xanthine oxidoreductase, mucin 15, CD 36, butyrophilin, adipose differentiation-related protein and fatty acid binding proteins, the content of the active ingredients in the milk powder is closer to the content of active ingredients in human milk, the constitution of the infant formula milk powder is optimized, and the formula milk powder obtained by using the method is closer to the human milk.

The invention discloses an infant formula milk powder for preventing infectious diarrhea, and belongs to the technical field of infant and young children formula milk powder. Each 100 g of the infant formula milk powder contains 250 mg-425 mg of free sialic acid and 30 mg-90 mg of milk fat globule membrane proteins. The invention provides the infant formula milk powder for preventing infectious diarrhea; for solving the risk of occurrence of infant infectious diarrhea, an appropriate amount of the high-quality free sialic acid and an appropriate amount of the milk fat globule membrane proteins are added and play a synergistic effect on preventing the occurrence of infant infectious diarrhea, and the occurrence of infant infectious diarrhea can be prevented.

The invention discloses infant formula goat milk powder added with lipidosome (rich in fat globule membranes and structural lipid OPO) simulating a fat structure of breast milk and a preparation method thereof. The infant formula goat milk powder comprises 0.16%-0.64% of MFGM proteins, 0.14%-0.56% of MFGM phospholipid and 3%-10.4% of structural lipid OPO. The infant formula goat milk powder adoptsdefatted goat milk as the raw material and is also added with the lipidosome which simulates the fat structure of the breast milk besides common nutritional ingredients. Fats in the formula milk power are formed by mixing and matching three types of vegetable oils and the OPO, and the compound oil package wrapped by MFGM is added into the defatted goat milk through a high-pressure micro-jet homogenization technology, thereby preparing the infant formula goat milk powder. By regarding the breast milk as a reference object and adding the milk fat globule membranes and the OPO into the formula powder, the infant formula goat milk powder has the nutritional ingredients, in particular, the fat globule composition and structure, closer to the breast milk, can promote brain and nerve developmentof newborns, can improve cognition and anti-infection abilities, can efficiently reduce a calcium soap constipation problem caused by palmitic acid in the traditional formula milk powder and is easyfor infants to digest and absorb.

The invention discloses a preparation method of mutton-smelling-free full fat goat milk powder reserving fatty acid of goat milk. The full fat goat milk powder comprises the following raw materials in parts by mass: 98.1-98.8 parts of fresh goat milk, 0.2-0.3 part of phospholipid, 0.8-1.2 parts of beta-cyclodextrine, 0.1-0.2 part of ascorbic acid, and 0.1-0.2 part of locust bean gum. Goat milk fat globule membranes are weak relatively, and are easy to destroy in various processing processes, so that the content of long chain fatty acid is reduced, the content of medium and short chain fatty acid is increased, and the mutton smelling of goat milk is increased. According to the method, the emulsification of phospholipid and the antioxidation of the ascorbic acid are used, so that the goat milk fat is protected; and besides, the entrapping effect of the beta-cyclodextrine is used, so that under the condition that the medium chain fatty acid is not removed, the mutton smelling is concealed, and the nutrient components of the goat milk are reserved to the maximum extent.

A method and composition for administering leptin to a subject. The invention includes suspending isolated native leptin-containing milk fat globules in a suitable medium for administering to a subject. The suspended milk fat globules may be administered orally as well as by intravenous, intramuscular, intraperitone41, other enteral routes of administration, and other parenteral routes of administration. The invention includes a method for treating growth or maturational-related disorders in newborns as, well as subjects having conditions that can be treated by the administration of leptin.

Provided are methods for preventing and treating inflammation and organ injury after ischemia / reperfusion, and methods for treating lung injury, comprising administering a milk fat globule epidermal growth factor-factor VIII (MFG-E8) to a subject. Also provided are pharmaceutical compositions comprising MFG-E8 in dosage form for preventing and treating inflammation and organ injury after ischemia / reperfusion, and for treating lung injury, and methods of preparing a pharmaceutical composition for preventing and treating inflammation and organ injury after ischemia / reperfusion, and for treating lung injury, comprising formulating MFG-E8 in a pharmaceutical composition.

The invention discloses a separation method of yak milk milk fat globule membrane protein, relates to a separation method of protein and solves problems of low purity and unstable properties in terms of cow's milk milk fat globule membrane protein prepared by existing methods. The separation method includes that cane sugar is added in yak milk, a milk fat initial extract is obtained through centrifugation, then centrifugal washing is performed with a sodion phosphate buffer solution and ultrapure water, and finally the centrifugation is performed with a polyethylene glycol octyl phenyl ether solution to obtain the yak milk milk fat globule membrane protein. The prepared yak milk milk fat globule membrane protein has the advantages that the properties are stable, the purity can reach to 93.5%, and problems of low purity and unstable properties in terms of the cow's milk milk fat globule membrane protein prepared by existing separation methods are solved. The separation method is applicable to mass production of the yak milk milk fat globule membrane protein.

Methods of treating cerebral ischemia by using milk fat globule epidermal growth factor-factor VIII (MFG-E8) are disclosed, as are recombinant human MFG-E8 and uses thereof in pharmaceutical compositions, products and methods for treating inflammation and organ injury after ischemia / reperfusion, sepsis, and lung injury.

The present invention provides a monoclonal and-idiotype antibody 11D10 that elicits an immune response against a specific epitope of a high molecular weight mucin of human milk fat globule (HMFG) and a hybridoma that produces 11D10. The hybridoma that produces 11D10 was selected by specific procedures. 11D10 inducts an Immunological response to HMFG in mice, rabbits, monkeys and patients with advanced HMFG-associated tumors. This invention provides compositions derived from polynucleotide sequences encoding the variable light and / or variable heavy regions of monoclonal anti-idiotype antibody 11D10, as well as polypeptides encoded thereby. The invention also provides compositions which can be used in the detection or treatment of HMFG-associated tumors.

Provided is a preparation method and device of personalized nano ice cream. Liquid nitrogen and ice cream raw materials are injected into a material container installed in a rotating-revolving mixer in a jet mode, the container containing the liquid nitrogen and ice cream raw materials performs high-speed revolution and rotation in a revolution track through the rotating-revolving mixer. Therefore, revolution energy having powerful accelerated speed is produced, meanwhile rotation makes the materials in the container to produce convection, accordingly full stirring, dispersion and defoaming within short time are achieved, and uniformly distributed nano ice crystals are efficiently formed. High-speed revolution and rotation mixing make the milk fat globules in the ice cream raw materials smaller and more uniformly emulsified, and finally a stable nano ice crystal-milk fat globule-microbubble structure is formed. The material container utilizes a container adapter for placement, common ice cream can be placed in a machine for direct processing, and container cleaning and waste are completely avoided. The production process is simple and stable, no stabilizer nor emulgator is needed in the preparation process, and the made ice cream has a fine and smooth taste and strong milk fragrance and is more natural.

The invention relates to infant formula milk powder enriched with milk fat globule membrane and structural grease OPO, as well as a preparation method thereof. The MFGM-protein content of the infant formula milk powder is 0.146-0.438%, and the structural grease OPO content is 2.07-5.17%. The preparation method of the infant formula milk powder enriched with the milk fat globule membrane and the structural grease OPO comprises the following steps: taking raw milk as the main raw material; adding MFGM-enriched whey protein powder, mixed vegetable oil (enriched with structural grease OPO), common whey protein powder, lactose, oligosaccharides, minerals, vitamins, and some selectable nutrients (including choline, inositol, taurine, carnitine, and so on); and carrying out batching standardization. The prepared infant formula milk powder has the effects of promoting growth and development of the infants. The infant formula milk powder is capable of achieving the purposes of promoting physical development and skeletal development of the infants, improving feeding comfort of the infants, as well as reducing incidence rates of intestinal diseases. The formula is suitable for development of formula milk powder for the infants and relatively larger infants.

The invention discloses a process for improving a homogenized milk fat globule membrane structure and components by using phospholipid, and belongs to the field of dairy product processing. Accordingto the invention, fresh milk and natural soybean phospholipid are used as main raw materials; according to the preparation method, liquid milk is obtained through burdening and homogenizing treatment,has smaller particle size and more complete phospholipid membrane structure, reduces whey protein and casein adsorption on the surface of homogenized milk fat globules, and contains MFGM functional proteins such as XDH, PASIII / III and the like in a higher proportion; the digestion amount of fat in the milk is increased by 9.6% compared with that of common homogenized milk, the change process of fat particles better conforms to the natural digestion state of fresh milk, and the purposes of improving the function of milk fat globule membrane MFGM protein and strengthening the nutrition of cow milk are achieved. The method is suitable for liquid milk, milk powder and other dairy products.

The present disclosure relates to a lipid source for nutritional compositions, comprising an enriched lipid fraction which comprises structured fat globules. The enriched lipid fraction provides fat globules having a desired size and fatty acid composition and may be stabilized by components such as phospholipids, cholesterol, milk-fat globule membrane protein and combinations thereof. Additionally, the disclosure relates to methods of supporting lipid digestion in a pediatric subject by providing a nutritional composition comprising an enriched lipid fraction having structured fat globules that are more accessible to lipases. The chemical composition, size and structure of the fat globules may improve digestion. The disclosed nutritional compositions may provide additive and or / synergistic beneficial health effects.

The invention discloses a method for embedding vitamin A acetic ester, relating to the method for embedding the vitamin A acetic ester using milk fat globule membrane phospholipid. The method of the invention comprises the following steps: adding the milk fat globule membrane phospholipid, cholesterol, the vitamin A acetic ester and vitamin E to an organic solvent, removing the organic solvent through rotary evaporation, adding imidazole buffer solution, processing by a high-speed cutter to obtain crude liposome suspension, and finally processing the obtained sample by a high-pressure homogenizer. The highest embedding rate of the liposome of the vitamin A acetic ester prepared by the method reaches up to 88%, which fills the gap in the prior art.

The present disclosure relates to a nutritional composition comprising a fat source that includes an enriched lipid fraction derived from milk. The enriched lipid fraction may contain saturated fatty acids, trans-fatty acids, monounsaturated fatty acids, CLAs, BCFAs, cholesterol, milk fat globule membrane proteins, gangliosides, and mixtures thereof. The enriched lipid fraction may exhibit additive or synergistic beneficial health effects when consumed. The disclosure further relates to methods of providing a nutritional composition comprising an enriched lipid fraction to targeted subjects.

The invention relates to an extraction method of a goat milk fat globule membrane protein for proteome research, and belongs to the technical field of milk fat globule membrane protein extraction. Themethod comprises the steps of adding cane sugar into a goat milk sample, performing high speed centrifugation to obtain crude separated milk fat, sequentially washing the crude extracted milk fat through a potassium phosphate buffer solution and ultrapure water, cracking through a lysis solution, and finally purifying through a precooled acetone solution, thus obtaining a milk fat globule membrane protein sample. According to the method provided by the invention, the influence of the low-content casein component can be effectively reduced, the extracted goat milk fat globule membrane proteinis higher in extraction rate and higher in stability, and the method has better popularization and application prospects and economic value in the goat milk fat globule membrane proteome research.

The present disclosure generally provides nutritional compositions that are useful for promoting beneficial bacteria in the gastrointestinal tract of a Cesarean-section (C-section)-delivered infant. The nutritional composition can include a prebiotic composition comprising human milk oligosaccharides (HMO), milk fat globule membrane (MFGM), and galacto-oligosaccharides (GOS) and / or polydextrose (PDX). The present disclosure also provides methods for promoting the growth of beneficial microbiota in the gastrointestinal tract of C-section-delivered infants comprising administering to a C-section-delivered infant the disclosed nutritional composition.

An immunochromatographic method for detecting a specific substance contained in milk, which comprises (1) the step of contacting the milk with a test strip having a first part retaining a labeled first antibody directed to the specific substance or the specific substance that is labeled, a second part disposed downstream from the first part, on which a second antibody directed to the specific substance is immobilized, and a third part disposed upstream from the first part or the second part and having voids enabling removal of milk fat globules contained in the milk, at the third part or a further upstream part, and (2) the step of flowing the milk up to the second part or a further downstream part to obtain a detectable signal of the label at the second part or a further downstream part.

The invention relates to the field of milk powder manufacturing, and in particular to novel milk powder and a manufacturing method thereof. The milk powder comprises the following components in partsby weight: OPO, whey protein powder (containing milk fat globule membranes and cephalin groups), raw milk, yak milk or whole yak milk powder, oligosaccharide, choline, DHA and lutein, and also comprises SN-2, wherein the content of the SN-2 is 60% or above of the weight of total palmitic acid in the milk powder. The OPO structural fat used in the novel milk powder is a new generation OPO, and thenovel milk powder has SN-2 close to the SN-2 level of breast milk, is beneficial to digestion and absorption of babies, and can improve energy intake, reduce constipation, enhance bone density, increase intestinal tract probiotics, reduce crying and improve night sleep. The novel milk powder can also improve cognitive ability, learning ability and memory of infants, can promote growth of probiotics, enhance the immune function of intestinal tracts, improve the stool character of the infants, reduce occurrence of constipation, and promote absorption and utilization of various nutrient substances.

An analogue peptide that comprises the variable regions of the light or heavy chains of an antibody of a first species selectively binding to a carcinoma antigen has 1 to 46 amino acids of the framework regions per chain substituted with amino acids such as those present in equivalent positions in antibodies of a species other than the first species, or fragments thereof comprising 1 to 3 variable region CDRs per chain and optionally flanking regions thereof of 1 to 10 or more amino acids, alone or with an N-terminal fragment of 1 to 10 or more amino acids, combinations or mixtures thereof. The polypeptide may also comprise an effector agent and / or be glycosylated, and is presented as a composition with a carrier. The analogue peptides are used in diagnostic kits for carcinomas and methods for in vivo imaging and treating a primary or metastasized carcinoma, and in vitro diagnosing a carcinoma, ex vivo purging neoplastic cells from a biological fluid. RNAs and DNAs encode the analogue peptide, and a hybrid vector carrying the nucleotides and transfected cells express the peptides and a method produces the analogue peptide. An anti-idiotype polypeptide comprises polyclonal antibodies raised against an anti-carcinoma antibody or the analogue peptide of this invention, monoclonal antibodies thereof, Fab, Fab′, (Fab′)2, CDR, variable region, or analogues or fragments thereof, combinations thereof with an oligopeptide comprising a TRP trimer, tandem repeats thereof, or combination or mixtures thereof. An anti-idiotype hybrid polypeptide with an effector agent and the anti-idiotype polypeptide, an anti-carcinoma vaccine, an anti-carcinoma vaccination kit, a method of vaccinating against carcinoma and a method of lowering the serum concentration of a circulating antibody or polypeptide are provided.

Methods of treating cerebral ischemia using milk fat globule epidermal growth factor-factor VIII (MFG-E8) are disclosed, as are recombinant human MFG-E8 and its uses in pharmaceutical compositions, products and methods for treating inflammation and organ injury after ischemia / reperfusion, sepsis, and lung injury.