A preparation method for an anti-aging biological cell preparation. The peripheral blood of healthy adults is used as a raw material for the anti-aging biological cell preparation which is prepared by the following steps: a, acquisition of the peripheral blood of healthy adults, which is used as the raw material for the preparation; b, separation and extraction of mononuclear cells in the peripheral blood; c, transformation and proliferation of the mononuclear cells after induction by cell mitogen in vitro; and d, detection of the obtained preparation. By being injected in a patient in an inoculation method of subcutaneous multi-point injection, the preparation of the invention can stimulate a body's immune cells, regulating immune function, effectively eliminate a sub-health state, delay dysfunction and be anti-aging, clinically achieving curative effects of adjusting the menstrual cycle, improving diet and sleep quality, promoting vitality and physical recovery, improving mental status, increasing disease resistance, making the skin bright and shiny, and maintaining a young appearance.