The invention discloses a preparation method of yolk wine, and belongs to the technical field of wine preparation. The preparation method comprises the following steps of: firstly, preparing yolk liquid; then, adding granulated sugar and an emulsifier into the yolk liquid, mixing and uniformly stirring; then, adding food-grade alcohol, brandy and essence into the yolk liquid, mixing, homogenizingfor a period of time, then filtering, filtering the prepared liquid by using a 100-mesh filter screen, retaining the filtrate, and discarding the filter residue; and finally, bottling the filtrate toobtain a finished product. The yolk wine prepared by the preparation method disclosed by the invention not only has the fragrant and sweet flavor of wine, but also retains the nutritional value of eggyolk, can supplement various trace elements for a human body by frequent drinking, can also clean the lipid peroxide in the body and prevent the generation of free radicals, and has great benefits for delaying human aging and prolonging human life. The yolk wine disclosed by the invention fills the blank in the field of prepared wine, enriches the product types, meets the market demand, and has good market prospects and economic benefits.