Buckwheat stress resistance and lodging resistance yield-increasing regulator and its preparation method and application

Abstract

The invention provides a buckwheat stress resistance and lodging resistance yield-increasing regulator and a preparation method and application thereof, belonging to the technical field of plant growth regulators. The buckwheat stress resistance and lodging resistance yield-increasing regulator uses water as a solvent and comprises the following components in concentrations: 7.5-10.5 g / L sodium p-chlorophenoxyacetate, 125-245 g / L cyprodinil, 70-200 g / L polyaspartate and 18-22 ml / L spreading agent. The buckwheat stress resistance and lodging resistance yield-increasing regulator can induce buckwheat to establish a huge root system, improve root system activity, increase the toughness of buckwheat stalks, reduce plant height, and improve buckwheat's ability to resist lodging; shorten branch length, improve canopy structure and function, improve buckwheat leaf photosynthetic performance and spike development; improve buckwheat stress resistance, improve pollen and stigma activity, extend the effective pollination time, and improve pollination rate and fruit setting rate; and promote buckwheat to obtain high and stable yields.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and in particular relates to a buckwheat stress resistance, lodging resistance and yield-increasing regulator, and a preparation method and application thereof. Background Art

[0002] Buckwheat belongs to the genus Fagopyrum of the family Leucaceae and is an annual herb. Buckwheat grows and develops rapidly, has a short growth period, and has high nutritional value in its grains. In agricultural production, it is often used as a disaster relief crop after wind, hail, and flood disasters. Buckwheat prefers a cool and humid environment to grow, is not resistant to high temperatures, drought, frost, and is prone to lodging. Therefore, buckwheat yields have always been at a low level, which has seriously affected the development of the buckwheat industry.

[0003] Buckwheat is widely distributed in my country, and is grown in the east, west, south and north. The main production areas are in the northwest, northeast, north China and the high-altitude mountainous areas in the southwest, with an annual planting area of ​​about 500,000 hectares. Buckwheat grains have high nutritional value. Its flour contains 13.55% protein, 2.5% fat and 72.2% carbohydrates, which are much higher than wheat, rice and corn. In addition, the main component of buckwheat protein is easily digestible globulin, and the lysine content in the protein is as high as 0.67-1.17% (close to eggs). The starch granules are small and easy to cook and digest. Therefore, the nutritional value index of buckwheat is as high as 80-90%. In addition, the dietary fiber content of buckwheat is 10 times that of refined rice, and the content of trace elements such as iron, manganese, zinc, and magnesium is much higher than that of general grains; and buckwheat contains B vitamins, vitamin E, amino acids, fatty acids, linoleic acid, nicotinic acid, niacin, rutin (rutin), flavonoids, etc., which have the medicinal effects of enhancing vascular elasticity, lowering blood lipids, promoting metabolism, preventing and treating diabetes, and relieving cough and asthma. Therefore, buckwheat is a crop that can be used as both medicine and food, and it is the most comprehensive and nutritious grain in terms of protein and amino acids. Not only that, buckwheat has a high feeding value. The straw contains 4.6% crude protein, 0.9% fat, and 19.5% carbohydrates. After being crushed, it is an excellent green fodder for poultry and livestock. Therefore, the buckwheat industry has good development prospects.

[0004] However, in field production, buckwheat has a low yield, with an average yield of about 85 to 100 kg per mu, which is only 1 / 10 to 1 / 5 of the average yield of staple food crops. Studies have found that in field production, buckwheat is prone to lodging due to its weak root system, shallow distribution, few fibrous roots, many canopy branches, and thin stems. Especially under unreasonable conditions of fertilization, irrigation, and planting density, it is very easy to cause large-scale lodging, resulting in low and unstable buckwheat yields. As the saying goes, "If you pour out a handful of chaff on the grain, you will pour out a handful of grass on the wheat." Buckwheat lodging mainly occurs during the grain filling period. After lodging, the plants are in a superimposed state, disrupting the spatial distribution structure of the leaves, destroying the aboveground group structure, causing ventilation and light transmission to be lost, and the lower functional leaves are completely lost, causing the leaves to wither and rot, and photosynthesis to decrease. At the same time, the internodes at the base are damaged, destroying the stem transportation function, and assimilation is blocked, which seriously inhibits the growth and development of the plant, causing the seeds to shrink and shrunken, and significantly reducing the number of grains per plant and the thousand grains, reducing the yield and quality of buckwheat; and bringing certain difficulties to the later harvest. In addition, the flowering, pollination and fruiting rate of buckwheat is very low, which affects the increase of buckwheat yield. Buckwheat has an indefinite growth habit, grows and develops rapidly, buds and blooms early, and has a short growing period; the growth of vegetative organs such as roots, stems, and leaves overlaps with the growth of reproductive organs such as budding, flowering, and filling for a long time, and the demand for carbohydrates is large. The photosynthetic products produced by its own limited leaf area cannot meet the needs of vegetative growth and reproductive growth, resulting in a high bud and flower abscission rate; and because the fertility of sweet buckwheat flowers is low, self-pollination and pollination of the same-type flowers in the two-type flowers cannot fertilize and bear fruit, and pollination between different-type flowers is required to bear fruit. Moreover, the vitality of pollen and stigma is low, the fertilization ability is poor, and the effective fertilization time is short, resulting in a large number of buckwheat flowers, but a very low pollination and fruiting rate, which is only about 10% of the flowering amount.

[0005] Therefore, buckwheat is prone to lodging and has a low fruit set rate, resulting in low and unstable yields, which seriously affects the development of the buckwheat industry.

[0006] Cultivation techniques such as selecting lodging-resistant varieties, timely sowing, reasonable density planting, reasonable nitrogen, phosphorus and potassium application, reasonable irrigation, scientific prevention and control of pests and diseases, furrow sowing and soiling can improve the lodging resistance of buckwheat to a certain extent and reduce production losses. However, the anti-lodging effect is not ideal, and the pollination rate of buckwheat cannot be improved, and the yield-increasing effect is not ideal; applying chemical regulation technology, spraying paclobutrazol, chlormequat, ethephon, etc. on the leaves of buckwheat from the seedling stage to the flowering stage, or soaking seeds with chlormequat and paclobutrazol to prevent excessive elongation of the internodes at the base, can reduce the occurrence of lodging by reducing plant height to a certain extent, but it also cannot effectively solve the problem of low pollination rate. In addition, ethephon, naphthylacetic acid, etc. can easily lead to an increase in the bud and flower abscission rate, resulting in a reduction in production. Therefore, developing special buckwheat-resistant and lodging-resistant yield-increasing regulators can reduce the lodging rate of buckwheat, increase the mechanical harvest rate of buckwheat, improve stress resistance, increase the pollination rate of buckwheat, increase the number of grains per plant, promote grain filling, and increase grain yield and quality on the basis of ensuring the normal growth and development of buckwheat. This is of great significance for the development of buckwheat specialty agricultural production. Summary of the invention

[0007] In view of this, the object of the present invention is to provide a buckwheat stress resistance and lodging resistance yield-increasing regulator with a scientific formula and convenient preparation, as well as a preparation method and application thereof. The buckwheat stress resistance and lodging resistance yield-increasing regulator can induce buckwheat to establish a huge root system, improve root system activity, increase the toughness of buckwheat stems, shorten the internode length of buckwheat, reduce plant height, and improve the ability of buckwheat to resist lodging; shorten branch length, improve canopy structure and function, improve the photosynthetic performance of buckwheat leaves and the development of spikes; improve buckwheat stress resistance, improve pollen and stigma activity, extend the effective pollination time, and improve the pollination rate and fruit setting rate; increase the number of grains per plant and the thousand-grain weight, and promote buckwheat to obtain high and stable yields.

[0008] The invention provides a buckwheat stress resistance and lodging resistance yield-increasing regulator, which takes water as a solvent and comprises the following components in concentrations: 7.5-10.5 g / L of sodium p-chlorophenoxyacetate, 125-245 g / L of cyprodinil, 70-200 g / L of polyaspartate and 18-22 ml / L of a spreader.

[0009] Preferably, the mass ratio of sodium p-chlorophenoxyacetate, cyprodinil and polyaspartate is 1:(11.90-23.33):(9.52-18.67).

[0010] Preferably, the spreading agent is selected from Triton and / or Tween substances.

[0011] Preferably, the Tween-like substance is selected from one or more of Tween 20, Tween 40, Tween 60, Tween 80 and Tween 85.

[0012] The present invention provides a method for preparing the buckwheat stress resistance and lodging resistance yield-increasing regulator, comprising the following steps:

[0013] After dissolving sodium chlorophenoxyacetate and polyaspartate in water, the mixture is mixed with a spreader to obtain a mixed solution; cyprodinil is dissolved in the mixed solution and waits for complete dissolution; and finally, water is added to make up the volume to obtain the mixture.

[0014] Preferably, the volume of water used to dissolve sodium chlorophenoxyacetate and polyaspartate is 30% to 70% of the total volume of water.

[0015] The invention provides the buckwheat stress resistance, lodging resistance and yield-increasing regulator, and application of the buckwheat stress resistance, lodging resistance and yield-increasing regulator prepared by the preparation method in buckwheat planting.

[0016] Preferably, the buckwheat stress resistance and lodging resistance yield-increasing regulator is diluted before use, and the dilution multiple is 500 to 700 times.

[0017] Preferably, the buckwheat stress resistance and lodging resistance yield-increasing regulator is used by seed soaking and / or foliar spraying.

[0018] Preferably, the foliar spraying is performed during the seedling stage and / or the flowering stage.

[0019] Compared with the prior art, the present invention has the following beneficial effects: the buckwheat stress resistance and lodging resistance yield-increasing regulator provided by the present invention is a product with the dual functions of enhancing stress resistance and lodging resistance and increasing yield, and has the characteristics of being non-toxic, having no side effects, being low in cost, and being easy to use. The buckwheat stress resistance and lodging resistance yield-increasing regulator can play multiple roles in one use, and compared with the existing plant growth regulators for adjusting buckwheat morphological indicators and yield indicators, it has the advantages of low investment, wide range of effects, and can comprehensively improve buckwheat stress resistance and lodging resistance, and increase pollination rate and yield.

[0020] The buckwheat stress resistance and lodging resistance yield-increasing regulator was used for multi-point demonstration test observations at the Touying Research Base of the Ningxia Hui Autonomous Region Academy of Agricultural and Forestry Sciences (Xuhe Village, Touying Town, Yuanzhou District, Guyuan City) and the Mahuangshan Buckwheat Demonstration Base of the Ningxia Hui Autonomous Region Academy of Agricultural and Forestry Sciences in Yanchang County (Huangyangling Village, Mahuangshan Township, Yanchang County, Wuzhong City). The results showed that after the application of the product, no lodging occurred in all the participating buckwheat fields in 2021 and 2022, and lodging occurred to varying degrees in the control fields, with the lodging rate ranging from 35% to 60%; after using the buckwheat stress resistance and lodging resistance yield-increasing regulator, the pollination rate and fruit setting rate were improved, and the yield increased by an average of more than 20%.

[0021] According to observations, after using the buckwheat stress resistance and lodging resistance yield-increasing regulator, the buckwheat plants underwent the following specific changes:

[0022] After use, the buckwheat root system will be huge, the main root will be deep, the secondary roots will be numerous and long, and the root system will be vigorous. It will increase the buckwheat root system's ability to fix the soil and absorb nutrients, making the seedlings strong, drought-resistant, low-temperature-resistant, and resistant to adversity, and lodging.

[0023] After use, the internode length of buckwheat stems is shortened, the plant height is reduced, the stems are thickened, the cellulose content of the stems is increased, and the buckwheat is resistant to lodging.

[0024] After use, the branches of buckwheat canopy are shortened and the permeability is enhanced; the leaves are broad, thick and dark green, photosynthesis is enhanced, and the photosynthetic functional period is extended; the flowering and pollination rate and fruiting rate are increased, promoting early maturity.

[0025] After use, buckwheat's stress resistance is improved, pollen and stigma vitality is improved, and the effective pollination period is extended; the pollination rate is increased, the number of grains per plant and the thousand-grain weight are increased, the quality of buckwheat is improved, and the yield is increased by more than 20%. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figures 1 to 3 This is a scanned root system image of buckwheat seeds soaked with the stress resistance and lodging resistance yield-increasing regulator in Example 10;

[0027] Figures 4 to 6 This is a scanned root system image after the seeds were soaked in clean water in Example 10. DETAILED DESCRIPTION

[0028] The invention provides a buckwheat stress resistance and lodging resistance yield-increasing regulator, which takes water as a solvent and comprises the following components in concentrations: 7.5-10.5 g / L of sodium p-chlorophenoxyacetate, 125-245 g / L of cyprodinil, 70-200 g / L of polyaspartate and 18-22 ml / L of a spreader.

[0029] In the present invention, the concentration of sodium p-chlorophenoxyacetate in the buckwheat stress resistance and lodging resistance yield-increasing regulator is 7.5-10.5 g / L, preferably 8-10 g / L, and more preferably 8.5-9.5 g / L. In the present invention, the sodium p-chlorophenoxyacetate accounts for 2.33% to 4.46% of the total solute. In the present invention, the sodium p-chlorophenoxyacetate can improve the toughness of the stems, promote the establishment of a huge root system, shorten the length of branches, and increase the lodging resistance of buckwheat. The source of the sodium p-chlorophenoxyacetate described in the present invention is not particularly limited, and conventional commercial products in this field can be used.

[0030] Sodium (4-chlorophenoxy) acetate (PCPA or 4-CPA): Other names: 4-chlorophenoxyacetic acid sodium, anti-drop agent, fruit setting agent, molecular formula C8H6ClNaO3, molecular weight 208.574, pure product is white needle-shaped or prismatic crystals, with a slight phenolic taste. It is easily soluble in water, and the aqueous solution has stable properties. Melting point 156.5℃, boiling point: 315.2℃ (at760mmHg), flash point 144.4℃. CAS No. 13730-98-8, EINECS No.: 237-299-4. Sodium (4-chlorophenoxy) acetate is a phenoxy plant growth regulator with auxin activity, which is absorbed by the roots, stems, leaves, flowers and fruits of plants. It can promote the synthesis of stem fiber, improve stem toughness, shorten the length of canopy branches, promote the construction of a large root system, and increase buckwheat's ability to resist lodging; it can promote the synthesis and transportation of plant photosynthetic products; it can prevent the formation of abscission layers, effectively prevent flowers and fruits from falling, increase the fruit setting rate of crops, and have the effect of increasing production and improving fruit quality. It is particularly effective for dicotyledonous plants. The oral LD50 of mice is 794mg / kg.

[0031] In the present invention, the concentration of cyprodinil in the buckwheat stress resistance and lodging resistance yield-increasing regulator is 125-245 g / L, preferably 130-200 g / L, and more preferably 140-175 g / L. In the present invention, cyprodinil plays the role of dwarfing plant height, improving buckwheat canopy light transmittance, improving leaf photosynthetic efficiency, promoting dry matter accumulation and distribution to grains, so as to promote flower organ development, promote grain filling, and increase thousand-grain weight.

[0032] Ancymidol (A-REST): Chemical name: α-cyclopropyl-α-(pyrimidin-5-yl)-4-methoxybenzyl alcohol, molecular formula: C 15 H 16 N2O2, molecular weight: 256.30, colorless crystal, insoluble in water, soluble in ethanol, ethyl acetate, chloroform and acetonitrile. The aqueous solution is stable at pH 7-11. Cyclopromazine inhibits the synthesis of gibberellins, slows down the growth rate of the aboveground vegetative part, inhibits the elongation of plant internodes, dwarfs plant height, promotes root development, increases the depth of the main root, and increases the number of lateral roots; increases the chlorophyll content of leaves, prolongs the functional period of leaves, promotes the synthesis of dry matter, can promote the development of buckwheat spike organs, promote flowering, and increase the fertilization rate of florets; improves buckwheat's resistance to lodging and increases yield. CAS number: 12771-68-5, EINECS number: 235-814-7.

[0033] In the present invention, the concentration of polyaspartate in the buckwheat stress resistance and lodging resistance yield regulator is 70-200 g / L, preferably 90-180 g / L, and more preferably 100-140 g / L. In the present invention, the polyaspartate is preferably a potassium polyaspartate solution, and the mass percentage of potassium polyaspartate in the potassium polyaspartate solution is 48.50%. In the present invention, the role of the polyaspartate is to improve buckwheat stress resistance, improve pollen and stigma activity, extend the effective fertilization time, improve pollination rate and fruit setting rate, reduce sterility rate, and increase the number of grains per plant and thousand-grain weight.

[0034] Polyaspartic acid is a specific protein polymerized from a single amino acid. It has peptide bonds and carboxylic acid groups in the molecule, has a strong chelating ability for ions, and is a biodegradable water-soluble polymer. The polyaspartic acid described in the present invention is a new type of green water treatment agent main component commonly found on the market, such as the polyaspartic acid (powder) product of Shandong Zibo Li Fine Chemical Co., Ltd., which contains more than 99.0% polyaspartic acid, and the ash, salt, and ammonia content are ≤1.0%. Polyaspartic acid (Polyaspartic acid, PASP): molecular formula C4H6NO3 (C4H5NO3) C4H6NO4, molecular weight 1000-5000, pure product is light brown crystals, insoluble in water, acetone, and ethyl acetate. During the growth and development of buckwheat, polyaspartic acid improves the physiological functions of cells by activating phosphodiesterase and regulating calcium metabolism. It improves the balance of endogenous hormones during the growth and development of buckwheat by inducing and regulating the second messenger system (calmodulin), thereby increasing the activity of protective enzymes in buckwheat leaves and flower organs, improving stress resistance, increasing division and stigma vitality, improving the photosynthetic performance of buckwheat leaves during the grain filling period, extending the photosynthetic life of leaves, and reducing the abortion rate. At the same time, it promotes the absorption and utilization of nitrogen by the roots, promotes protein synthesis, and improves the quality of buckwheat grains.

[0035] In the present invention, the concentration of the spreading agent in the buckwheat stress resistance and lodging resistance yield-increasing regulator is 18-22 ml / L, preferably 19-21 ml / L, and more preferably 20 ml / L. In the present invention, the spreading agent is preferably selected from Triton and / or Tween substances, and further preferably selected from one or more of Tween 20, Tween 40, Tween 60, Tween 80 and Tween 85.

[0036] In the present invention, sodium p-chlorophenoxyacetate, cyprodinil and polyaspartic acid act synergistically, have the functions of dwarfing plant height, improving stem toughness, shortening branch length, promoting root development and increasing buckwheat lodging resistance; have the functions of improving buckwheat canopy structure and function, promoting flower organ development and grain filling, and increasing thousand-grain weight; have the functions of improving buckwheat stress resistance, increasing pollination rate and fruit setting rate, and increasing the number of grains per plant and thousand-grain weight.

[0037] The present invention also provides a method for preparing the buckwheat stress resistance and lodging resistance yield-increasing regulator, comprising the following steps:

[0038] After dissolving sodium chlorophenoxyacetate and polyaspartate in water, the mixture is mixed with a spreader to obtain a mixed solution; cyprodinil is dissolved in the mixed solution and waits for complete dissolution; and finally, water is added to make up the volume to obtain the mixture.

[0039] In the present invention, the volume of water used to dissolve sodium chlorophenoxyacetate and polyaspartate is preferably 30% to 70% of the total volume of water, and more preferably 40% to 60%.

[0040] The present invention also provides the buckwheat stress resistance, lodging resistance and yield-increasing regulator, and application of the buckwheat stress resistance, lodging resistance and yield-increasing regulator prepared by the preparation method in buckwheat planting.

[0041] In the present invention, the buckwheat stress resistance and lodging resistance yield-increasing regulator is preferably diluted before use, and the dilution multiple is preferably 500 to 700 times, and more preferably 550 to 650 times.

[0042] In the present invention, the buckwheat stress resistance and lodging resistance yield-increasing regulator is preferably used by seed soaking and / or foliar spraying.

[0043] In the present invention, the seed soaking is preferably carried out before sowing, and the buckwheat stress resistance and lodging resistance yield-increasing regulator is preferably diluted before seed soaking, and the dilution multiple is preferably 500 to 700 times, and more preferably 550 to 650 times. In the present invention, the mass ratio of seeds to the diluted buckwheat stress resistance and lodging resistance yield-increasing regulator is preferably 1:0.7 to 0.9, and more preferably 1:0.8; after the seeds and the buckwheat stress resistance and lodging resistance yield-increasing regulator are mixed, the seeds are soaked at room temperature for 10 to 14 hours, preferably 12 hours; after the seed soaking is completed, the buckwheat stress resistance and lodging resistance yield-increasing regulator is almost completely absorbed by the seeds, and before sowing, the seeds are spread out in a cool place to dry, and can be used for sowing.

[0044] In the present invention, the period of foliar spraying is preferably the seedling stage and / or the flowering stage; the present invention has no limitation on the method and specific dosage of foliar spraying, and it is advisable to spray the leaves until they are moist without dripping according to conventional spraying methods in the art.

[0045] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0046] The plant growth regulators of the present invention are selected from potassium polyaspartate (Shandong Zibo Libang Fine Chemical Co., Ltd., registration certificate number: Q / ZLB-002-2005), cyprodinil (Henan Yuanqing Biotechnology Co., Ltd., pesticide registration certificate number: PD20180827) and sodium p-chlorophenoxyacetate (Sichuan Guoguang Agrochemical Co., Ltd., registration certificate number: PD20151572), and Tween 20 is used as a spreader.

[0047] Example 1

[0048] To 500 ml of water, add 100 g of polyaspartate and 7.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 125 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0049] Example 2

[0050] To 500 ml of water, add 100 g of polyaspartate and 10.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 125 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0051] Example 3

[0052] To 500 ml of water, add 100 g of polyaspartate and 7.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 175 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0053] Example 4

[0054] To 500 ml of water, add 100 g of polyaspartate and 10.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 175 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0055] Example 5

[0056] To 500 ml of water, add 140 g of polyaspartate and 7.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 125 g of cyclopentane. After dissolution, dilute to 1000 ml with water.

[0057] Example 6

[0058] To 500 ml of water, add 140 g of polyaspartate and 10.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 125 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0059] Example 7

[0060] To 500 ml of water, add 140 g of polyaspartate and 7.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 175 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0061] Example 8

[0062] To 500 ml of water, add 140 g of polyaspartate and 10.5 g of sodium p-chlorophenoxyacetate. After complete dissolution, add 20 ml of Tween 20 to the above solution, and then add 175 g of cyprodinil. After dissolution, dilute to 1000 ml with water.

[0063] Example 9

[0064] In the specific implementation, the three components have a significant synergistic effect. Select potassium polyaspartate, cyprodinil and sodium p-chlorophenoxyacetate as the main components, set three concentration gradients of 0, 1, and 2 respectively (see Table 1 for concentration gradients), perform permutations and combinations, a total of 26 treatments and 1 control, and use the sweet buckwheat variety Xinnong No. 1 as the test variety. Spray the leaves during the seedling and flowering stages, repeat three times, harvest at maturity, and measure yield. As shown in Table 2, the results show that the three components have a significant synergistic effect, plant height is reduced by 1.9%, branch number is increased by 10.7%, harvest density is increased by 2.4%, plant grain number is increased by 57.8%, thousand-grain weight is increased by 2.3%, and yield is increased by 65.3%.

[0065] Table 1 Concentration gradient of potassium polyaspartate, cyprodinil and sodium p-chlorophenoxyacetate (concentration: ppm)

[0066] Code Potassium polyaspartate(P) Cyprodinil (A) Sodium 4-chlorophenoxyacetate (C) 0 0 0 0 1 100 250 15 2 200 500 30

[0067] Table 2 Synergistic effect experimental results of potassium polyaspartate (P), cyprodinil (A) and sodium p-chlorophenoxyacetate (C)

[0068]

[0069]

[0070] Example 10

[0071] Effect of buckwheat seed soaking with stress and lodging resistance regulator on buckwheat morphological indicators

[0072] 1. Experimental scheme: 60 healthy seeds of mature, plump and uniform size were randomly selected, first disinfected with 10% sodium hypochlorite for 10 minutes, and then 30 seeds were grouped and soaked in 500-fold dilution of buckwheat stress resistance and lodging increase regulator (prepared in Example 1) and clean water for 12 hours, and then the surface moisture of the seeds was dried with absorbent paper after being taken out. Then, they were placed on the median position of the vertical glass plate, each vertical plate was paved with 2 layers of wet filter paper, and the seeds were placed between the two filter papers, and then gently compacted with a glass rod to make the seeds in close contact with the filter paper; the vertical plate was inserted into the water tank so that its bottom was placed below the water surface, and the water level in the tank was maintained at 5 cm to ensure that the filter paper continued to absorb water upward and keep the filter paper moist. 10 seeds were placed on each plate, repeated 3 times, and cultured at a constant temperature of 25°C. After 10 days of culture, the root length, the number of lateral roots, the stem thickness and the seedling height were measured.

[0073] Buckwheat seedlings were transplanted into plastic pots (specifications of 27cm*20cm*22cm), with 5 plants in each pot and 6kg of soil in the pot. The soil was a mixture of black soil and vermiculite in a volume ratio of 4:1. From transplanting to the peak flowering period of buckwheat, the soil moisture content in the pot was maintained at 70% of the field water holding capacity by weighing method, that is, weighing once every 5 days to replenish the water lost by transpiration from the buckwheat leaves. Root samples were taken during the peak flowering period, and the roots were scanned with a root scanner to study root traits such as the number, length, surface area of ​​roots, and the number, length, and surface area of ​​secondary roots of different diameters. In order to avoid root overlap, squeezing, and masking, the roots were dismembered before being scanned. The black-and-white photos of the roots taken by the scanner after dismemberment are shown below. Figures 1 to 3 and Figures 4 to 6 As shown in the photo, the shape, size, number and length of the main root and secondary root are clearly shown. Figures 1 to 3 This is a scanned root system image of buckwheat seeds soaked with stress resistance, lodging resistance and yield-increasing regulator; Figures 4 to 6 This is a scanned root system image after the seeds were soaked in clean water.

[0074] 2. Test results

[0075] The experiment showed that the buckwheat stress resistance and lodging resistance yield regulator treatment promoted root development, reduced seedling height, increased stem thickness, and improved the quality of buckwheat seedlings. Among them, the length of the main root and the number of lateral roots of buckwheat increased by 7.65% and 41.48%, the stem thickness increased by 14.92%, and the seedling height decreased by 39.39%.

[0076] Table 3 The regulatory effect of buckwheat seed soaking treatment with stress and lodging resistance regulators on buckwheat morphological indicators

[0077] Morphological indicators Comparison deal with Increase / decrease% Root length (cm) 53.07±1.49b 57.13±2.47a 7.65 Number of lateral roots 25.07±1.87b 35.47±1.68a 41.48 Stem diameter (cm) 2.48±0.17b 2.85±0.16a 14.92 Seedling height (cm) 96.47±4.73b 58.47±3.31a -39.39

[0078] Embodiment 11

[0079] Foliar spray conditioner

[0080] The regulator is sprayed on the leaves during the buckwheat seedling and flowering stages. When applied, the regulators of Examples 1, 2, 5, and 6 are diluted with water to 500 times, and the regulators of Examples 3, 4, 7, and 8 are diluted with water to 700 times, and then sprayed on the leaves.

[0081] Example 12

[0082] Effects of buckwheat stress and lodging resistance regulators on pollination rate, fruit setting rate, empty rate and yield of buckwheat

[0083] 1. Experimental scheme: The buckwheat stress resistance and lodging resistance and yield-increasing regulator provided by the present invention was used for field trials in Touying Research Base of Ningxia Academy of Agricultural and Forestry Sciences (Xuhe Village, Touying Town, Yuanzhou District, Guyuan City) in 2021 and 2022. The Xinnong No. 1 sweet buckwheat variety was used as the experimental material, and the solution diluted 500 times in Example 1 was sprayed on the leaves at the seedling stage and flowering stage, respectively. The foliar spraying of clean water was used as a control. The total number of flowers, total number of grains, number of filled grains, and number of empty grains were investigated and counted at the flowering stage (0, 5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 days after flowering) and the maturity stage, and the pollination rate, fruit setting rate, and empty rate were calculated. The experiment adopted a randomized block design with three repetitions. The plot is 6m long, 3m wide, and covers an area of ​​18m 2 ; Each plot is planted with 6 rows, 0.5m spacing, and a density of 66,600 plants / mu; artificial spot sowing, sowing depth of 3-5cm, sowing dates are July 17, 2021 and July 18, 2022 respectively; nitrogen, phosphorus and potassium fertilizers are applied as a one-time basal fertilizer, 1500kg / mu of decomposed farmyard manure, 10kg / mu of urea, 11.5kg / mu of P2O5, and 12kg / mu of K2O. Field management is the same as conventional fields.

[0084] Artificial assisted pollination: During the peak flowering period, artificial assisted pollination is performed using the rope pulling method. A long strip of linen that is the same length as the test plot and 10-15 cm wide is fixed on a rope. From 10 a.m. to 5 p.m. on a sunny day, two people pull the two ends of the rope and walk back and forth along the edge of the plot 2-3 times. When walking, let the linen strip brush the top inflorescence of the buckwheat flower, causing it to shake. This can be done once every 3-4 days during the peak flowering period, and 3-4 times during the entire growth period.

[0085] Statistics of pollination rate, fruit setting rate and empty grain rate: One representative sampling point was selected in each plot for treatment and control. Starting from the first day of flowering, 15 plants were continuously surveyed at each sampling point, and the number of primary branches, number of flower clusters, total number of flowers, total number of grains, number of filled grains and number of empty grains were counted.

[0086] Pollination rate = total number of seeds / total number of flowers;

[0087] Fruit setting rate = number of seeds / total number of flowers;

[0088] Empty rate = number of empty grains / total number of grains

[0089] Yield: One representative sample point was selected from each plot for treatment and control, and 1 m 2 , equivalent to per mu yield (666.67m 2 calculate).

[0090] 2. Test results:

[0091] The results of the field test in 2021-2022 showed that the treatment of buckwheat stress resistance and lodging resistance increased pollination rate, fruit setting rate and grain yield. As shown in Tables 4, 5 and 7, the treatment of buckwheat stress resistance and lodging resistance increased pollination rate and fruit setting rate, and increased yield; among them, the pollination rate and fruit setting rate increased by an average of 11.36-13.70 percentage points and 8.43-11.60 percentage points respectively, and the average yield increased by 29.59%-33.13%.

[0092] Table 4 The regulatory effect of buckwheat stress and lodging resistance regulators on the pollination rate of buckwheat

[0093]

[0094]

[0095] Pollination rate = total number of seeds / total number of flowers

[0096] Table 5 The effect of buckwheat stress resistance and lodging resistance regulator treatment on buckwheat fruit setting rate

[0097]

[0098]

[0099] Fruit setting rate = number of seeds / total number of flowers

[0100] Table 6 The effect of buckwheat stress resistance and lodging resistance regulator treatment on buckwheat shriveling rate

[0101]

[0102]

[0103] Empty rate = number of empty grains / total number of grains;

[0104] Table 7 The regulatory effect of buckwheat stress resistance and lodging resistance regulator treatment on buckwheat yield

[0105]

[0106]

[0107] 2. The effect of buckwheat stress resistance and lodging resistance yield regulator on buckwheat yield and lodging rate (field demonstration)

[0108] 1. Experimental scheme: The products provided by the present invention are respectively arranged for field demonstration in 2021 and 2022 at the Touying Research Base of the Ningxia Hui Autonomous Region Academy of Agricultural and Forestry Sciences (Xuhe Village, Touying Town, Yuanzhou District, Guyuan City) and the Mahuangshan Buckwheat Demonstration Base of the Ningxia Hui Autonomous Region Academy of Agricultural and Forestry Sciences in Yanchi County (Huangyangling Village, Mahuangshan Township, Yanchi County, Wuzhong City), with a demonstration area of ​​50 mu; Xinnong No. 1 sweet buckwheat variety is used as the experimental material, and the 500-fold diluted solution of Example 1 is set to be sprayed on the leaves at the seedling stage and flowering stage respectively, and the foliar spraying of clean water is used as the control. The buckwheat stress resistance and lodging resistance yield regulator treatment demonstration area is 30 mu, and the clean water control demonstration is 20 mu. The yield is measured at five points in the demonstration area, and the yield measurement point area is 10m 2 , count the number of harvested plants and the number of lodging plants, and mechanically harvest and measure yield. Sowing on July 10, 2021, seedling stage on July 16, full flowering stage on August 20, harvest stage on October 2, growth period of 78 days; sowing on July 18, 2022, seedling stage on July 24, full flowering stage on August 26, harvest stage on October 3, growth period of 71 days.

[0109] Lodging rate = number of lodging plants / number of harvested plants.

[0110] 2. Test results:

[0111] The results of field demonstration from 2021 to 2022 showed that the treatment of buckwheat stress resistance and lodging resistance yield-increasing regulator reduced the lodging rate and increased the yield; among them, the lodging rate was reduced by 35.20%-60.04% (see Table 9 and Table 11), and the average yield per mu increased by 20.34%-39.33% (see Table 8 and Table 10).

[0112] Table 8 The regulatory effect of buckwheat stress and lodging resistance regulators on buckwheat yield (Touying base, Yuanzhou District, Guyuan City)

[0113]

[0114]

[0115] Table 9 The effect of buckwheat stress resistance and lodging resistance regulator treatment on buckwheat lodging rate (Touying base, Yuanzhou District, Guyuan City)

[0116]

[0117]

[0118] Table 10 The regulatory effect of buckwheat stress and lodging resistance regulators on buckwheat yield (Yanchi County Mahuangshan Demonstration Base)

[0119]

[0120] Table 11 The effect of buckwheat stress resistance and lodging resistance regulator on buckwheat lodging rate (Yanchi County Mahuangshan Demonstration Base)

[0121]

[0122] It can be seen from the above embodiments that the sodium p-chlorophenoxyacetate, cyprodinil and polyaspartic acid in the buckwheat stress resistance and lodging resistance yield-increasing regulator provided by the present invention act synergistically, have the functions of dwarfing plant height, shortening branch length, promoting root development and increasing buckwheat lodging resistance; have the function of increasing thousand-grain weight; have the function of improving buckwheat stress resistance, increasing pollination rate and fruit setting rate, and increasing the number of grains per plant and thousand-grain weight.

[0123] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A buckwheat stress resistance and lodging resistance yield-increasing regulator, characterized in that: The invention uses water as solvent and comprises components with the following concentrations: 7.5-10.5 g / L sodium p-chlorophenoxyacetate, 125-245 g / L cyprodinil, 70-200 g / L polyaspartate and 18-22 ml / L spreading agent; the spreading agent is selected from Triton and / or Tween substances.

2. The buckwheat stress resistance and lodging resistance yield-increasing regulator according to claim 1, characterized in that The mass ratio of sodium p-chlorophenoxyacetate, cyprodinil and polyaspartate is 1:(11.90~23.33):(9.52~18.67).

3. The buckwheat stress resistance and lodging resistance yield-increasing regulator according to claim 1, characterized in that The Tween-like substance is selected from one or more of Tween 20, Tween 40, Tween 60, Tween 80 and Tween 85.

4. The method for preparing the buckwheat stress resistance and lodging resistance yield-increasing regulator according to any one of claims 1 to 3, characterized in that: The following steps are involved: After dissolving sodium chlorophenoxyacetate and polyaspartate in water, the mixture is mixed with a spreader to obtain a mixed solution; cyprodinil is dissolved in the mixed solution and waits for complete dissolution; and finally, water is added to make up the volume to obtain the mixture.

5. The preparation method according to claim 4, characterized in that: The volume of water used to dissolve sodium chlorophenoxyacetate and polyaspartate is 30% to 70% of the total volume of water.

6. Use of the buckwheat stress resistance, lodging resistance and yield-increasing regulator according to any one of claims 1 to 3 or the buckwheat stress resistance, lodging resistance and yield-increasing regulator prepared by the preparation method according to claim 4 or 5 in buckwheat planting.

7. The use according to claim 6, characterized in that: The buckwheat stress resistance and lodging resistance yield-increasing regulator is diluted before use, and the dilution multiple is 500 to 700 times.

8. The use according to claim 7, characterized in that: The buckwheat stress resistance and lodging resistance yield-increasing regulator is used by seed soaking and / or foliar spraying.

9. The use according to claim 8, characterized in that: The period for foliar spraying is the seedling stage and / or the flowering stage.

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