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Nymphaea hybrid polysaccharide and preparation method thereof

A technology for perfume lotus and polysaccharide, which is applied in the directions of antitoxins, skin diseases, drug combinations, etc., can solve the problems of high extraction temperature, uneven color, low extraction efficiency, etc., and achieves the effects of good moisturizing, high purity, uniform and stable color

Inactive Publication Date: 2021-03-30
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation method of lotus flower polysaccharide in perfume is mainly alcohol precipitation after boiling water extraction. This method has high extraction temperature, low extraction efficiency, great damage to the polysaccharide structure, low purity and uneven color, which cannot meet people's needs for the wide application of its polysaccharide.
In previous studies, Sevage method and trichloroacetic acid method were used to remove protein from lotus polysaccharides, which resulted in the residue of highly toxic reagents such as chloroform and trichloroacetic acid, and the loss of polysaccharides was high.
Studies have shown that the hydrogen peroxide method has a significant decolorization effect on polysaccharides. This method will cause the degradation of the molecular weight of polysaccharides while oxidizing pigments, and is not suitable for the preparation of high molecular weight perfume lotus polysaccharides
The current research on lotus polysaccharides in perfume mainly focuses on low and medium molecular weight polysaccharides of 200-400kDa, and there are few studies on high molecular weight polysaccharides. In the separation and purification, DEAE-52 and Sephadex G-150 chromatographic columns were used for separation, and the polysaccharides were obtained with high purity, but The yield is low, the production cost is high, and the steps are cumbersome, so it is not suitable for industrial production

Method used

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  • Nymphaea hybrid polysaccharide and preparation method thereof
  • Nymphaea hybrid polysaccharide and preparation method thereof
  • Nymphaea hybrid polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 prepares macromolecule perfume lotus polysaccharide from perfume lotus:

[0037] (1) Perfume lotus is crushed and passed through a 40-mesh sieve, and 200 g of perfume lotus powder is weighed and placed in 600 mL of ethanol with a volume fraction of 90%, heated to reflux at 70 ° C for 3 h, filtered through filter paper, and the filter residue is dried in an oven at 40 ° C for 12 h. Get Perfume Lotus Powder.

[0038] (2) Accurately weigh 100g of perfume lotus powder, the mass ratio of perfume lotus powder to deionized water is 1:30, after 200W ultrasonication for 30min, heat and extract in a water bath at 70°C for 4h, the speed of the stirring paddle is 200rpm, after gauze filtration, Concentrate to 300mL under reduced pressure at 40°C with a rotary evaporator to obtain a concentrated perfume lotus crude polysaccharide extract.

[0039] (3) The AB-8 macroporous resin was soaked in deionized water for 6 hours, then soaked in ethanol with a volume fraction of ...

Embodiment 2

[0041]Embodiment 2 prepares the perfume lotus polysaccharide from the perfume lotus:

[0042] (1) Perfume lotus is crushed and passed through a 40-mesh sieve, weighed 200g of perfume lotus coarse powder and placed in 600mL of ethanol with a volume fraction of 90%, heated to reflux at 80°C for 3h, filtered through filter paper, and the filter residue was dried in an oven at 40°C for 12h. Get Perfume Lotus Powder.

[0043] (2) Accurately weigh 100g of perfume lotus powder, the mass ratio of perfume lotus powder to deionized water is 1:40, after 250W ultrasonication for 25min, heat and extract in a water bath at 80°C for 3h, the speed of the stirring paddle is 200rpm, after gauze filtration, Concentrate to 300mL under reduced pressure at 40°C with a rotary evaporator to obtain a concentrated perfume lotus crude polysaccharide extract.

[0044] (3) The AB-8 macroporous resin was soaked in deionized water for 6 hours, then soaked in ethanol with a volume fraction of 70% for 6 hour...

Embodiment 3

[0046] Embodiment 3 prepares the perfume lotus polysaccharide from the perfume lotus:

[0047] (1) Perfume lotus is crushed and passed through a 40-mesh sieve, and 400g of perfume lotus powder is weighed and placed in 1600mL of ethanol with a volume fraction of 90%, heated to reflux at 70°C for 3h, filtered through filter paper, and the filter residue is dried in an oven at 40°C for 10h. Get Perfume Lotus Powder.

[0048] (2) Accurately weigh 200g of perfume lotus flower powder, the mass ratio of perfume lotus flower powder to deionized water is 1:50, after 300W ultrasonication for 25min, heat and extract in a water bath at 70°C for 3h, the speed of the stirring paddle is 200rpm, after gauze filtration, Concentrate under reduced pressure at 60°C to 400mL in a rotary evaporator to obtain a concentrated extract of crude polysaccharide from lotus flower.

[0049] (3) The AB-8 macroporous resin was soaked in deionized water for 6 hours, then soaked in ethanol with a volume fracti...

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Abstract

The invention discloses nymphaea hybrid polysaccharide and a preparation method thereof. The preparation method comprises the following steps that 1, crushing and sieving the nymphaea hybrid to obtainnymphaea hybrid coarse powder, adding ethanol into the nymphaea hybrid coarse powder, performing heating reflux, and drying filter residues to obtain nymphaea hybrid powder; 2, adding water into nymphaea hybrid powder, carrying out ultrasonic treatment and heating and stirring extraction, then filtering the extract, and carrying out reduced pressure concentration to obtain a concentrated nymphaeahybrid crude polysaccharide solution; 3, adding pretreated macroporous resin into the nymphaea hybrid crude polysaccharide solution, carrying out heating and stirring for adsorption, carrying out suction filtration to obtain a decolorized and deproteinized polysaccharide solution, and carrying out vacuum concentration to obtain a concentrated sugar solution; and 4, adding ethanol into the concentrated sugar solution for precipitation, centrifuging the liquid, collecting the precipitate, and carrying out vacuum drying to obtain nymphaea hybrid polysaccharide. The extraction rate is high, the molecular weight of the obtained polysaccharide is high and is about 1100 Kda, and the polysaccharide is faint yellow in appearance, high in purity and uniform and stable in color and luster and has good moisture retention, oxidation resistance and anti-inflammatory activity.

Description

technical field [0001] The invention relates to the extraction of natural active substances, and provides a perfume lotus flower polysaccharide and a preparation method thereof. Background technique [0002] Perfume lotus is an aquatic perennial herb of the Nymphaeaceae family. At present, there are nine colors of gold, purple, blue, yellow, white, and red, so it is also known as the nine-pin perfume lotus. At present, there are planting bases in Zhuhai, Guangdong, Liuzhou, Guangxi, Fujian, Sichuan and other places, with rich resources. However, except that the perfume lotus is used as an ornamental, its main product is only perfume lotus tea. Studies have shown that the perfume lotus has the functions of lowering blood fat, anti-oxidation, whitening, and protecting the liver. The edible safety of the perfume lotus has also been confirmed. Deep mining of functional ingredients can increase the added value of the lotus perfume. [0003] The polysaccharide content in the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P17/00A61P17/18
CPCC08B37/0003A61P39/06A61P17/00A61P17/18
Inventor 谭诗敏程建华罗志刚周心慧
Owner SOUTH CHINA UNIV OF TECH
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