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Detection method for pollen viability of nymphaea hybrid

A technology for perfume lotus and pollen viability, which is applied to the measurement of pollen germination ability and the detection of perfume lotus pollen viability, can solve problems such as large differences and uncertainty, and achieve the effects of simple method, reliable data and strong operability.

Pending Publication Date: 2019-08-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are studies on the pollen viability of cold-resistant water lilies of the same genus, they have not been systematically designed to thoroughly explore the effects of different medium treatments on the germination rate of water lily pollen. Whether the culture combination is the best and whether it can be widely used cannot be Sure
At the same time, the culture medium, culture conditions and culture time of in vitro culture are

Method used

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  • Detection method for pollen viability of nymphaea hybrid
  • Detection method for pollen viability of nymphaea hybrid
  • Detection method for pollen viability of nymphaea hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Taking the 'yellow flower type' perfume lotus as an example, collect its pollen during the best collection period, put the pollen into a centrifuge tube, and store it in the dark at 4°C for later use; 1% solid culture medium is used as nutrient matrix, carries out sucrose, boric acid and temperature three-factor orthogonal experiment, carries out pollen viability measurement according to the method provided by the present invention with the collected ' yellow flower type ' perfume lotus pollen, the obtained ' yellow flower type ' perfume The test results of the lotus pollen culture medium are shown in Table 1. The results show that: the culture medium of 50g / L sucrose+10mg / L boric acid+20mg / L calcium chloride+10g / L agar, cultured at 30°C, the 'yellow flower type' perfume Lotus pollen has the highest vitality, with an average vitality of 60.00%.

[0066]

[0067] Table 1

Embodiment 2

[0069] Taking the 'pink flower type' perfume lotus as an example, collect its pollen during the best collection period, put the pollen into a centrifuge tube, and store it in the dark at 4°C for later use; The 1% solid culture medium is used as the nutrient matrix, and the three-factor orthogonal test of sucrose, boric acid and temperature is carried out, and the pollen vigor is measured with the collected 'pink flower type' perfume lotus pollen according to the method provided by the invention, and the obtained 'pink flower Type 'perfume lotus pollen culture medium test result is as shown in table 2, and the result shows: the culture medium of 50g / L sucrose+10mg / L boric acid+20mg / L calcium chloride+10g / L agar, cultivates under 30 ℃, 'powder The flower type 'perfume lotus' has the highest pollen vigor, with an average vigor of 56.67%.

[0070]

[0071]

[0072] Table 2

Embodiment 3

[0074] Taking the 'Purple Flower Type' perfume lotus as an example, collect its pollen during the best collection period, put the pollen into a centrifuge tube, and store it in the dark at 4°C for later use; 1% solid culture medium is used as the nutrient matrix, and the three-factor orthogonal experiment of sucrose, boric acid and temperature is carried out, and the pollen vigor measurement is carried out according to the method provided by the present invention with the collected 'purple flower type' perfume lotus pollen, and the obtained 'purple flower type' perfume The test results of the lotus pollen culture medium are shown in Table 2. The results show that: the culture medium of 50g / L sucrose+10mg / L boric acid+20mg / L calcium chloride+10g / L agar, cultured at 30°C, 'purple flower type' perfume Lotus pollen has the highest vigor, with an average vigor of 48.26%.

[0075]

[0076] table 3

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Abstract

The invention discloses a detection method for pollen viability of nymphaea hybrid. The method comprises the following steps: 1, collecting pollen of the nymphaea hybrid in the optimum collecting period of the pollen of the nymphaea hybrid, putting in a centrifuge tube, and keeping in dark place at 4 DEG C for standby use; 2, preparing a solid medium with distilled water, wherein the solid mediumcontains saccharose, boric acid, agar, and calcium chloride; 3, dipping a drop of an unconsolidated solid medium with a glass bar and placing in the spherical concave surface of a concave glass slide,and keeping flat; standing, and then dipping the pollen of the nymphaea hybrid with a brush pen and spreading uniformly on a solidified medium; then placing the concave glass slide in a petri dish spread with wet filter paper, placing in an artificial incubator for constant-temperature and dark-box culture; 4, after completing the culture, placing the concave glass slide under a microscope for observing, and recording the number of germinated pollen and the sum of pollen grains. The detection method disclosed by the invention is scientific, reasonable, and simple, and is strong in operability; complete quantitative detection on the pollen viability of the nymphaea hybrid of large tropical nymphaea tetragona.

Description

technical field [0001] The invention relates to a method for measuring the germination ability of pollen, in particular to a method for detecting the vigor of perfumed lotus pollen, and belongs to the technical field of plant hybridization and breeding. Background technique [0002] Perfume lotus (Nymphaea hybrid), an aquatic bulbous flower of the Nymphaea family, has huge flowers, beautiful flower shapes, and strong fragrance. Now it mainly has nine colors such as gold, yellow, red, purple, blue, red, white, tea, and green. Therefore, it is also known as "Nine Pin Perfume Lotus". It is suitable for tropical to temperate climates. Under suitable temperature conditions, it can bloom uninterrupted all year round and can be viewed all year round. It has a wide range of functions, not only can be used for potted plants, gardens, garden landscaping, etc., but also has practical value in the fields of medicine, industry, food, perfume, cosmetics, etc. and medicinal value. [00...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5097
Inventor 祝遵崚张慧会司宇火艳
Owner NANJING FORESTRY UNIV
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