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Method for efficiently collecting and storing nymphaea hybrid anther and pollen

A perfume lotus and pollen technology, applied in the field of plant development biology, can solve the problems of high storage cost, less pollen quantity, pollen loss, etc., and achieve the effect of avoiding cell damage, ensuring objectivity, and spending less time

Inactive Publication Date: 2019-06-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preliminary experiments found that in the natural state, the pollen life of the perfumed lotus flower is very short, which brings great disadvantages to germplasm preservation and hybrid breeding.
[0004] For the preservation of plant pollen, the method of ultra-low temperature is currently used, but this method not only requires high storage conditions and high storage costs, but also requires a long pollen recovery process and complicated technology when pollen is needed. Browning of pollen, loss of activity of pollen protease, the pollen preserved by this method cannot be used immediately, and the use efficiency is low
[0005] Some methods for maintaining the vitality of pollen are also disclosed in the prior art, but the storage period is short, which cannot fully meet the actual needs of water lily hybrid breeding work and germplasm resource preservation research.
At the same time, the pollen of the perfume lotus is small and the amount of pollen is small, so it is difficult to collect a large amount of pollen. If the current method of collecting and storing plant pollen is continued, it will not only take time and labor, but also easily lead to excessive loss of pollen.

Method used

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  • Method for efficiently collecting and storing nymphaea hybrid anther and pollen
  • Method for efficiently collecting and storing nymphaea hybrid anther and pollen
  • Method for efficiently collecting and storing nymphaea hybrid anther and pollen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Take the 'yellow flower type' perfume lotus as an example

[0038] (1) Pretreatment of anthers and pollen: collect perfumed lotus flowers in the big bud stage, take out the anthers of perfumed lotus, set aside anthers in a single layer and spread them evenly on sulfuric acid paper or parchment paper, the thickness of anthers and pollen tiled is 0.6cm, and then put the single-layer anthers and scattered pollen in a square drawer with a size of 45cm*40cm*20cm for 20 hours in shading and drying. Use an external air conditioner to ensure that the dry environment in the square drawer is room temperature 22-28°C, relative humidity 60 %~70%. After drying, the water content of the lotus anthers and pollen of the 'yellow flower type' perfume is 6.5%; the silica gel color-changing desiccant is spread in the square drawer, and the thickness of the silica gel color-changing desiccant is 3 cm, which is placed on sulfuric acid paper or parchment paper When the silica gel discolorati...

Embodiment 2

[0045] Take the 'purple flower type' perfume lotus as an example

[0046] (1) Pretreatment of anthers and pollen: collect perfumed lotus flowers in the big bud stage, take out the anthers of perfumed lotus, set aside anthers in a single layer and spread them evenly on sulfuric acid paper or parchment paper, the thickness of anthers and pollen tiled is 0.4cm, and then put the single-layer anthers and scattered pollen in a square drawer with a size of 45cm*40cm*20cm for 23 hours in shading and drying. Use an external air conditioner to ensure that the dry environment in the square drawer is room temperature 22-28°C, relative humidity 60 %~70%. After drying, the water content of the lotus anthers and pollen of the 'Purple Flower Type' perfume is 4.5%; the silica gel color-changing desiccant is spread in the square drawer, and the thickness of the silica gel color-changing desiccant is 2 cm, which is placed on sulfuric acid paper or parchment paper When the silica gel discolorati...

Embodiment 3

[0053] Take the 'pink flower type' perfume lotus as an example

[0054] (1) Pretreatment of anthers and pollen: collect perfumed lotus flowers in the big bud stage, take out the anthers of perfumed lotus, set aside anthers in a single layer and spread them evenly on sulfuric acid paper or parchment paper, the thickness of anthers and pollen tiled is 1cm, and then put the single-layer anthers and scattered pollen in a square drawer with a size of 45cm*40cm*20cm to dry for 24 hours. Use an external air conditioner to ensure that the dry environment in the square drawer is room temperature 22-28°C and relative humidity 60%. ~70%. After drying, the water content of the lotus anthers and pollen of the 'pink flower type' perfume is 5%; the silica gel color-changing desiccant is tiled in the square drawer, and the thickness of the silica gel color-changing desiccant tiles is 2.5 cm, which is placed on sulfuric acid paper or Under the parchment paper, when the silica gel color-changi...

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PUM

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Abstract

The invention discloses a method for efficiently collecting and storing nymphaea hybrid anther and pollen. The method includes the steps of firstly, preprocessing anther and pollen; secondly, storingthe anther and pollen; thirdly, unfreezing the anther and pollen and conducting pollination. A great amount of pollen can be efficiently collected, the vitality of the pollen is effectively maintained, the pollination rate is increased by naturally separating the anther and pollen in the pollination process, the method has the advantages that the spent time is short, the pollen loss is low and thepollen vitality is high, the service life of the nymphaea hybrid pollen is prolonged, and the pollen utilization rate is remarkably increased.

Description

technical field [0001] The invention relates to a method for preserving plant pollen, in particular to a method for efficiently collecting and preserving perfume lotus anthers and pollen, belonging to the field of plant developmental biology. Background technique [0002] Perfume lotus (Nymphaea hybrid) is a large tropical water lily of the Nymphaeaceae family, mainly distributed in Guangzhou, Fujian, Hainan, Sichuan, Zhejiang and other places in my country. Because of its rich flower colors and beautiful flower shapes, it is widely used in fresh cut flowers, garden water lake landscaping and potted plants. At the same time, many studies have shown that the perfume lotus has anti-oxidation, inhibition of prostatic hyperplasia, lowering blood lipids, etc., and has a certain purification and repair effect on air, water and soil pollution. It can be seen that the perfume lotus is a kind of aquatic flower plant integrating medicinal, ecological and ornamental application values...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 祝遵崚张慧会火艳
Owner NANJING FORESTRY UNIV
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