Skin whitening and moisturizing mask containing nymphaea hybrid extractives and preparing method thereof
A technology for perfume lotus and extracts, which is applied in the field of daily chemicals to achieve excellent skin feel, excellent antioxidant effect and remarkable effect.
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Embodiment 1
[0021] Example 1: Preparation method of perfume lotus total polysaccharide
[0022] Take the dried perfume lotus ovary and crush it, sieving it through a 40-mesh sieve, weighing 200 g, adding 25 times the amount of water to hot soaking in a water bath at 100°C for 4 to 5 hours, pre-filtering with gauze and centrifuging. The drug residue was extracted twice with the same method. The three supernatants were combined and concentrated under reduced pressure on a rotary evaporator at 70°C to 1 / 5 of the original volume. 95% ethanol was added to the concentrated solution for precipitation. When the ethanol concentration reached At 60% (volume content), a large amount of flocculent precipitation appears, let stand for 24h, and centrifuge. The precipitate was washed with absolute ethanol, acetone, and ether in sequence, and dried in vacuum to obtain the total polysaccharide of Perfume lotus.
Embodiment 2
[0023] Example 2: Preparation method of total flavonoids of perfume lotus
[0024] Take the dried scent lotus with stamen ovary and crush it, sieving through a 40-mesh sieve, weighing 200 g, degreasing with petroleum ether twice, 2 h each time, and filtering. After drying the filter residue, it was extracted twice with 75% ethanol at reflux for 3 h each time. After suction filtration, the extracts were combined, concentrated under reduced pressure to a material-liquid ratio of 1:2, and separated with HPD-400 macroporous resin. The eluents were 30% and 60% ethanol. The eluate of the 60% ethanol fraction was concentrated under reduced pressure and dried under vacuum to obtain the total flavonoids of perfume lotus.
Embodiment 3
[0025] Example 3: Preparation method of natural β-glucan
[0026] Natural β-glucan is prepared from the extracellular polysaccharide produced by Agrobacterium ZX09. For its preparation method, please refer to the patent (Patent No.: 201010146371.2; Inventor: Zhang Jianfa, Xiu Aihui, Zhu Bin; Patent Name: Agrobacterium ZX09, Water-soluble natural β-glucan produced by Agrobacterium ZX09 and its preparation method and its application in lowering blood sugar) Example 3 in the specification.
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