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Nymphaea hybrida polysaccharide extract as well as preparation method and application thereof

A technology for extracting perfume lotus and polysaccharides, which can be used in applications, polysaccharide/gum-containing food ingredients, pharmaceutical formulations, etc. It can solve the problems of unsatisfactory properties, polysaccharide structure breakage, denaturation, etc., and achieve pure white color, convenient operation, and stable properties Effect

Pending Publication Date: 2021-09-17
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of extraction and purification of lotus polysaccharides in perfume, the current existing technology relies on the adsorption of macroporous resins, and the prepared polysaccharides have obvious color, so it is easy to change the color after long-term storage, which will trouble the follow-up of related products, especially whitening products. to develop
At present, the extraction of polysaccharides from lotus flower of perfume generally adopts high temperature and ultrasonic-assisted extraction methods. However, under the action of high temperature or ultrasonic waves, the polysaccharide structure is prone to breakage and denaturation.
[0005] At the same time, the extraction of polysaccharides from the lotus flower of the perfume is mainly concentrated in crude extracts or high molecular weight polysaccharides. The content of polysaccharides is not high or the properties cannot meet the needs of subsequent product development.

Method used

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  • Nymphaea hybrida polysaccharide extract as well as preparation method and application thereof
  • Nymphaea hybrida polysaccharide extract as well as preparation method and application thereof
  • Nymphaea hybrida polysaccharide extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, the preparation of perfume lotus flower polysaccharide extract

[0021] Get freeze-dried perfume lotus 100g, pulverize through a grinder, and the pulverized powder is filtered through a 20-order iron sieve to obtain perfume lotus micropowder. Add 10 times the volume (1L) of water to the perfumed lotus micropowder, and extract in a water bath with mechanical stirring (400 rpm) for 4-5 hours at room temperature. The extract was initially filtered through gauze, and the filtrate was collected. The dregs were extracted twice in the same way, and the filtrates were combined three times. The filtrate was concentrated to 1 / 10 (100mL) of the original volume through a rotary evaporator again, and a volume fraction 95% ethanol solution (130mL) was added to the concentrated solution, and stirred to precipitate, when the added ethanol concentration reached 60% ( volume content), a large number of flocculent precipitates appeared. After the precipitate was filtered ...

Embodiment 2

[0025] Embodiment 2: the antibacterial activity evaluation of compound

[0026] In this embodiment, the minimum inhibitory concentration (MIC) of the antibacterial activity of the sample will be determined by the resazurin chromogenic method. The experimental strains were Methicillin-resistant Staphylococcus aureus (MRSA, methicillin-resistant Staphylococcus aureus), Staphylococcus aureus (SA, Staphylococcus aureus), Bacillus cereus (BC, Bacillus cereus), B.subtilis (BS, Gram-positive or negative bacteria such as Bacillus subtilis), B.thuringiensis (BT, Bacillus thuringiensis) or Escherichiacoli (EC, Escherichia coli).

[0027] In the test, the 96-well plate dilution titer technique will be used to simultaneously determine the minimum inhibitory concentration (MIC) of various substances. Firstly mix 7.5mL indicator solution (100μg / mL resazurin aqueous solution) with 5mL test bacteria solution (10 8 CFU / mL) Mix well, and add 100 μL of the mixed bacterial solution to all the t...

Embodiment 3

[0031] Example 3: Determination of Inhibiting Lipid Peroxidation Activity

[0032] Weigh 0.50 mL lecithin solution and add it into 1.0 mL PBS buffer solution with pH=7.0. 1.0mL of sample solutions containing different concentrations were uniformly mixed with 1.0mL of an aqueous solution containing 2.5mmol / L EDTA-Fe(II), and then added to the aforementioned PBS mixed solution of lecithin. After mixing, place it in a water bath at 37°C for 45 minutes. Then, 2.0 mL of 28% (w / v) trichloroacetic acid and 1.0 mL of 1% (w / v) thiobarbituric acid were added successively. After mixing, the mixed solution was heated in a boiling water bath at 100°C for 10 min, and the absorbance was measured at 532 nm after cooling. Use PBS buffer to adjust to zero, and replace the sample with PBS buffer to measure the absorbance A0 of the blank tube. Make 3 parallel samples for each sample, and take the average value A1. Calculate the lipid peroxidation inhibition rate according to the following for...

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Abstract

The invention discloses a nymphaea hybrida polysaccharide extract as well as a preparation method and application thereof. The preparation method comprises the steps of crushing nymphaea hybrida, adding water for extraction, and concentrating an extracting solution to obtain an extract; adding an ethanol solution into the extract, and conducting stirring, filtering, precipitating, freezing and drying to obtain a polysaccharide crude extract I; dissolving the polysaccharide crude extract I in water, conducting deproteinizing by using a Sevag method, washing out a precipitate, conducting filtering, and drying the precipitate to obtain a polysaccharide crude extract II; removing monosaccharide and oligosaccharide: dissolving the polysaccharide crude extract II in water, conducting dialyzing by using a dialysis membrane with the cutoff molecular weight of 8000-14000 D, adding an ethanol solution into the dialyzed polysaccharide crude extract liquid, conducting stirring until the precipitate is sucked out, conducting filtering, and drying the precipitate to obtain a polysaccharide crude extract III; and conducting gel column purification: purifying the polysaccharide crude extract III by using a gel chromatographic column, tracking fractions by using a sulfuric acid-phenol method, collecting main peaks, conducting merging, adding an ethanol solution, and conducting stirring, filtering, precipitating, and freeze-drying to obtain the nymphaea hybrida polysaccharide extract. The nymphaea hybrida polysaccharide extract has the effects of resisting bacteria, whitening, moisturizing and resisting oxidation.

Description

Technical field: [0001] The invention belongs to the field of plant extraction, and in particular relates to a perfume lotus flower polysaccharide extract and its preparation method and application. Background technique: [0002] Perfume lotus is a large water lily, which belongs to the perennial aquatic perennial herbaceous plant of Nymphaeaceae. Perfume lotus has an elegant fragrance and a long flowering period. It is the most high-end variety of urban garden waterscapes, courtyard landscapes, and potted ornamental rare aquatic flowers at home and abroad. In the 1970s, American fragrant lotus was introduced to Taiwan, and on this basis, nine color systems were developed through cultivation, including gold, yellow, purple, blue, red, tea, green, red, and white, so it is also known as "Nine Grades". Perfume lotus". Perfume lotus is suitable for tropical to temperate climates, so it is widely planted in Guangdong, Hunan, Zhejiang, Jiangsu, Hainan and other places in my coun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A23L2/52A23L33/125A61Q17/00A61Q19/00A61Q19/02A61Q19/08
CPCC08B37/0003A61K8/73A61Q19/08A61Q19/02A61Q17/005A61Q19/00A23L2/52A23L33/125A61K2800/805A61K2800/84A23V2002/00A23V2250/51A23V2200/30
Inventor 谭海波崔博张文歌
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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