31results about How to "Guaranteed not to be degraded" patented technology

The invention discloses a method for fast determination of algae species and quantity by employing rRNA-targeted S1 enzyme protection probe and sandwich hybridization technique, which comprises, (1) mixing and hybridizing the detected sample with S1 enzyme protection analyzing probe, (2) treating the mixed solution in step (1) through S1 enzyme digestion, (3) carrying out denaturation treatment to release S1 enzyme protection probe, (4) trapping residual S1 enzyme protection analyzing probes with trapping probes fixed on the solid-phase carriers, (5) hybridizing the linking probe with S1 enzyme protection analyzing probe, then hybridizing with the signal probe with detectable signals, (6) detecting the detectable signals to determine the algae species and / or quantity in the sample.

A Schizochytrium limacinum strain detection probe group belongs to the field of nucleic acid detection. The invention includes three strips of interrelated oligonucleotide probes: an analysis probe for specific binding of rRNA of the Schizochytrium limacinum (S.limacinum), a capture probe for binding on a vector and specific binding with the analysis probe, and a signal probe for specific binding with the analysis probe. The analysis probe has a nucleotide sequence shown as in a SEQ ID NO:1; the capture probe has a sequence shown as in a SEQ ID NO:2 and has biotin labeling; and a signal probe has a sequence as shown in a SEQ ID NO:3 and has an isothiocyanic acid (FITC) label at the 5' terminal. The detection probe group is in linear correlation (R2=0.9942) with detection signal strength when cell concentration of S.limacinum is within 0.29*10<3>-2.1*10<3> in per milliliter of nutrient solution, has the advantages of good specificity, high sensitivity, high stability, qualitative and quantitative research, and is used for quality inspection during breeding and fermentation production process of the S.limacinum strain.

A probe composition group for detecting Pseudomonas bacteria belongs to the technical field of nucleic acid detection. The probe composition is composed of three oligonucleotide probes. An analysis probe has as a nucleotide sequence shown in SEQ ID NO:1; a capture probe has a nucleotide sequence shown in SEQ ID NO:2, and has biotin label at the 3' terminal; a signal probe has a nucleotide sequence shown in SEQ ID NO: 3, and has isothiocyanic acid label (FITC) at the 5' terminal. The probe composition has bioinformatics specificity, is able to distinguish Pseudomonas, Comamonas, Aeromonas, Acinetobacter, Bacillus subtilis and Escherichia coli; the probe has quantitative linear range of 7.08*10<3> -6.31*10<6> cells / mL, and the linear equations are as below: y=0.373x-0.962, R<2>=0.996. The probe composition can be used for qualitative and quantitative analysis of Pseudomonas bacteria.

The invention relates to a detection probe of a group of bacillus ((i) Bacillus ( / i)) strains and belongs to the field of nucleic acid detection. The detection probe comprises three oligonucleotide probes which are correlated with one another, namely, an analysis probe which is specifically bonded with 16SrRNA (Ribose Nucleic Acid) of the bacillus, a capture probe which is bonded on a carrier and specifically bonded with the analysis probe, and a signal probe which is specifically bonded with the analysis probe, wherein the analysis probe has the nucleotide sequence shown in SEQ ID NO:1; the capture probe has a nucleotide sequence which is shown in SEQ ID NO:2 and provided with biotin marks; the signal probe has the nucleotide sequence shown in SEQ ID NO:3; and the 5' end of the nucleotide sequence of the signal probe is provided with fluorescein isothiocyanate (FITC) marks. If the strain concentration of the (i) B.subtilis ( / i) strains in a culture solution per milliliter is within 1.33*10<2>- 2.13*10<5>, the strain concentration is linearly correlated with the strength of detection signals, wherein R2=0.99741. In addition, the detection probe is good in specificity, high in sensitivity, high in stability, qualitative and quantitative. Thus, the detection probe can be applied to the real-time monitoring of the bacillus during the traditional fermentation production process of white wine and the like.

The invention discloses a drug-containing esomeprazole pellet and a preparation method thereof. Expressed in percent by weight, the esomeprazole drug-containing pellets mainly consist of 25-50% of raw materials esomeprazole and its derivatives, 20-50% of mannitol and 5-30% of crospovidone. % composition; In addition, it also contains pharmaceutically acceptable pharmaceutical excipients. The invention carries out proportioning according to the raw material composition of the esomeprazole drug-containing pellets, and utilizes an extrusion-spheronization preparation method to produce the product of the invention, the esomeprazole drug-containing pellets. Compared with the prior art, the technical scheme of the present invention is used to prepare esomeprazole drug-containing pellets, which improves efficiency and avoids wasting a lot of time, manpower and material resources; and the method is simple, and the obtained product preparation intermediate has the The advantages of stability and high reproducibility.

A detection probe for Prorocentrum micans Ehrenberg belongs to the nucleic acid detection field. It is used for S1 enzyme protection analysis and sandwich hybridization detection of the Prorocentrum micans Ehrenberg, and comprises three correlative oligonucleotide probes. Wherein a S1 enzyme protection analysis probe only serves as a complement of the nucleotides in the Prorocentrum micans Ehrenberg ribosome big subunit 430~520 regions, and shows no homology with other Prorocentrum micans Ehrenberg ribosome RNAs. The S1 enzyme protection analysis probe has a length of 58bp, and can be combined specifically onto the rRNAs of the Prorocentrum micans Ehrenberg under certain conditions, and the sequence is: 5'-ACCAGGCACATTCACCCACCCAGGGGTAGGCTACCATGTCCCTGGAGTTTTCCTCGAT. The invented probes have excellent applicability to make it convenient to seek and realize the specific probe. The invention has higher stability than that of the sandwich hybridization technology.

This invention discloses a pyramidal Si alga test probe, which belongs to nucleic acid test field and comprises the following steps: to integrate a group of probes centered as Si enzyme protective probe in the specific area of rRNA; to combine the Si enzyme protective analysis and clamping crossbreed technique to realize the ration measurement of the pyramidal Si alga. The said probes comprise three connected oligonucleotide probes, Si enzyme protective probe, catching probe, and signal probes or connection probe.

A detection probe for Prorocentrum minimum belongs to the nucleic acid detection field. It is used for S1 enzyme protection analysis and sandwich hybridization detection of the Prorocentrum minimum. and comprises three correlative oligonucleotide probes. Wherein a S1 enzyme protection analysis probe only serves as a complement of the nucleotides in the Prorocentrum minimum ribosome big subunit 430~520 regions, and shows no homology with other Prorocentrum minimum ribosome RNAs. The S1 enzyme protection analysis probe has a length of 58bp, and can be combined specifically onto the rRNAs of the Prorocentrum minimum under certain conditions, and the sequence is: 5'-ACAGTCCGCAAATGAGTTCTGCCAAGGCTATTCACTCACCCGTAGACGAGCTACCATGA. The invented probes have excellent applicability to make it convenient to seek and realize the specific probe. The invention has higher stability than that of the sandwich hybridization technology.

A detecting probe of brown colpomenia for protecting and analyzing the S1 enzyme of said seaweed and detecting it by sandwich hybridization has mutually correlative three oligonucleotide probes including the S1 enzyme protecting and analyzing probe.

The invention relates to a thiobacillus ferrooxidans detection probe composition comprising an analysis probe used to be specifically combined with the rRNA of the thiobacillus ferrooxidans, a capturing probe used to be combined on a carrier and is used to be specifically combined with the analysis probe, and a signal probe used to be specifically combined with the analysis probe. The signal probe has protein markers. Preferably, the analysis probe has a nucleotide sequence represented by SEQ ID No. 1, the capturing probe has a nucleotide sequence represented by SEQ ID No.2 and has biotin markers, and the signal probe has a nucleotide sequence represented by SEQ ID No. 3. The protein markers are fluorescein markers. The invention also provides a thiobacillus ferrooxidans detection method. The thiobacillus ferrooxidans detection probe composition provided by the invention is inelegantly designed. With the detection probe composition, thiobacillus ferrooxidans can be rapidly quantitatively and qualitatively detected. The detection method has the advantages of simple operation and good repeatability. The method is suitable for large-scale popularization.

A detection probe for Heterosigma akashiwo belongs to the nucleic acid detection field. It is used for S1 enzyme protection analysis and sandwich hybridization detection of the Heterosigma akashiwo, and comprises three correlative oligonucleotide probes. Wherein a S1 enzyme protection analysis probe only serves as a complement of the nucleotides in the Heterosigma akashiwo ribosome small subunit 150~300 regions, and shows no homology with other Heterosigma akashiwo ribosome RNAs. The S1 enzyme protection analysis probe has a length of 59bp, and can be combined specifically onto the rRNAs of the Heterosigma akashiwo under certain conditions, and the sequence is: 5'-GGCAGAAACTTGAATGAACCATCGACCGAAGTCGATTCGCACAGTTACTATGATTCACC. The invented probes have excellent applicability to make it convenient to seek and realize the specific probe. The invention has higher stability than that of the sandwich hybridization technology.

The invention discloses a detection probe for Skeletons costa, which belongs to the field of nucleic acid detection. A set of probes with the S1 enzyme protection analysis probe as the core was synthesized specifically for the specific region of the rRNA of S. costa, combined with the S1 enzyme protection analysis and sandwich hybridization technology to realize the qualitative and quantitative detection of the rRNA of S. costa. The detection probes include three interrelated oligonucleotide probes: S1 enzyme protection analysis probe, capture probe, signal probe or connection probe. The probe of the invention has good applicability and greatly facilitates the search and realization of the specific probe. The stability of the present invention is much higher than that of the sandwich hybridization technique.