31results about How to "Guaranteed not to be degraded" patented technology

The invention discloses a method for fast determination of algae species and quantity by employing rRNA-targeted S1 enzyme protection probe and sandwich hybridization technique, which comprises, (1) mixing and hybridizing the detected sample with S1 enzyme protection analyzing probe, (2) treating the mixed solution in step (1) through S1 enzyme digestion, (3) carrying out denaturation treatment to release S1 enzyme protection probe, (4) trapping residual S1 enzyme protection analyzing probes with trapping probes fixed on the solid-phase carriers, (5) hybridizing the linking probe with S1 enzyme protection analyzing probe, then hybridizing with the signal probe with detectable signals, (6) detecting the detectable signals to determine the algae species and/or quantity in the sample.

The invention provides pepper oil resin which comprises the following components by mass fraction: 45-48% of piperine and 24-28% of pepper oil. The preparation method of the resin comprises the steps of breaking dried pepper fruits to obtain ground pepper, mixing with cellulase and pectinase for enzymolysis, obtaining an enzymolysis product, mixing the enzymolysis product with an alcohol aqueous solution, extracting, condensing and drying. The contents of the pepper oil and piperine in the pepper oil resin are higher; the method is mild in condition; the quality of the pepper oil resin is improved; the yield of the pepper oil resin is increased; flavor components of the pepper are completely extracted; in addition, no toxic and harmful organic solvent is residual in the pepper oil resin; and the purity of the pepper oil resin is improved.

This invention discloses a pyramidal Si alga test probe, which belongs to nucleic acid test field and comprises the following steps: to integrate a group of probes centered as Si enzyme protective probe in the specific area of rRNA; to combine the Si enzyme protective analysis and clamping crossbreed technique to realize the ration measurement of the pyramidal Si alga. The said probes comprise three connected oligonucleotide probes, Si enzyme protective probe, catching probe, and signal probes or connection probe.

A detection probe for Prorocentrum micans Ehrenberg belongs to the nucleic acid detection field. It is used for S1 enzyme protection analysis and sandwich hybridization detection of the Prorocentrum micans Ehrenberg, and comprises three correlative oligonucleotide probes. Wherein a S1 enzyme protection analysis probe only serves as a complement of the nucleotides in the Prorocentrum micans Ehrenberg ribosome big subunit 430~520 regions, and shows no homology with other Prorocentrum micans Ehrenberg ribosome RNAs. The S1 enzyme protection analysis probe has a length of 58bp, and can be combined specifically onto the rRNAs of the Prorocentrum micans Ehrenberg under certain conditions, and the sequence is: 5'-ACCAGGCACATTCACCCACCCAGGGGTAGGCTACCATGTCCCTGGAGTTTTCCTCGAT. The invented probes have excellent applicability to make it convenient to seek and realize the specific probe. The invention has higher stability than that of the sandwich hybridization technology.

The invention discloses salivary DNA preservation liquid, which is prepared from the following component in the following content: 0.1 to 8 percent (w/v) of SDS. The salivary DNA preservation liquid disclosed by the invention is simple in formula, and is used for preserving salivary DNA samples of a human body and can preserve the salivary DNA for a long time at room temperature, and the DNA in the preserved saliva is high in integrity; the salivary DNA preservation liquid can ensure the stability of transportation of a saliva sample between various collection sites and is applicable to gene detection and relevant scientific researches.

The invention provides a preserving fluid for preserving bacterial DNA in an excrement sample at normal temperature. The preserving fluid comprises sodium chloride, absolute ethyl alcohol, a biological bacteriostatic agent, disodium EDTA, and deionized water. The preserving fluid comprises the following components: 0.5-2 mol/L of the sodium chloride, 20-60% (v/v) of the absolute ethyl alcohol, 0.01-0.2% (v/v) of the biological bacteriostatic agent, 10-100 mmol/L of the disodium EDTA, and the balance of the deionized water. The pH value of the preserving fluid is 7-8, and the biological bacteriostatic agent is Proclin 300. According to the preserving fluid for preserving the bacterial DNA in the excrement sample at normal temperature, the situation that the DNA of microorganisms in the excrement sample is not degraded in a normal-temperature environment can be guaranteed, the number, the composition and the abundance of the microorganisms in the excrement sample are kept stable throughgood storage performance, and the accuracy of data analysis after intestinal flora sequencing is guaranteed; and the normal-temperature preservation and the transportation of the excrement sample areachieved, the preserving fluid is suitable for long-distance transportation, and compared with an existing preserving fluid on the market, the preserving fluid has the advantages of being good in preservation effect, easy to operate, good in applicability and free of toxicity and harm.

The invention discloses a nymphaea hybrida polysaccharide extract as well as a preparation method and application thereof. The preparation method comprises the steps of crushing nymphaea hybrida, adding water for extraction, and concentrating an extracting solution to obtain an extract; adding an ethanol solution into the extract, and conducting stirring, filtering, precipitating, freezing and drying to obtain a polysaccharide crude extract I; dissolving the polysaccharide crude extract I in water, conducting deproteinizing by using a Sevag method, washing out a precipitate, conducting filtering, and drying the precipitate to obtain a polysaccharide crude extract II; removing monosaccharide and oligosaccharide: dissolving the polysaccharide crude extract II in water, conducting dialyzing by using a dialysis membrane with the cutoff molecular weight of 8000-14000 D, adding an ethanol solution into the dialyzed polysaccharide crude extract liquid, conducting stirring until the precipitate is sucked out, conducting filtering, and drying the precipitate to obtain a polysaccharide crude extract III; and conducting gel column purification: purifying the polysaccharide crude extract III by using a gel chromatographic column, tracking fractions by using a sulfuric acid-phenol method, collecting main peaks, conducting merging, adding an ethanol solution, and conducting stirring, filtering, precipitating, and freeze-drying to obtain the nymphaea hybrida polysaccharide extract. The nymphaea hybrida polysaccharide extract has the effects of resisting bacteria, whitening, moisturizing and resisting oxidation.

The invention relates to a detection probe of a group of bacillus ((i) Bacillus (/i)) strains and belongs to the field of nucleic acid detection. The detection probe comprises three oligonucleotide probes which are correlated with one another, namely, an analysis probe which is specifically bonded with 16SrRNA (Ribose Nucleic Acid) of the bacillus, a capture probe which is bonded on a carrier and specifically bonded with the analysis probe, and a signal probe which is specifically bonded with the analysis probe, wherein the analysis probe has the nucleotide sequence shown in SEQ ID NO:1; the capture probe has a nucleotide sequence which is shown in SEQ ID NO:2 and provided with biotin marks; the signal probe has the nucleotide sequence shown in SEQ ID NO:3; and the 5' end of the nucleotide sequence of the signal probe is provided with fluorescein isothiocyanate (FITC) marks. If the strain concentration of the (i) B.subtilis (/i) strains in a culture solution per milliliter is within 1.33*10<2>- 2.13*10<5>, the strain concentration is linearly correlated with the strength of detection signals, wherein R2=0.99741. In addition, the detection probe is good in specificity, high in sensitivity, high in stability, qualitative and quantitative. Thus, the detection probe can be applied to the real-time monitoring of the bacillus during the traditional fermentation production process of white wine and the like.

The invention discloses a zearalenone detoxifying agent and a preparation method thereof, and belongs to the field of additives. The zearalenone detoxifying agent includes bacillus subtilis HDR-036, and the bacillus subtilis HDR-036 is preserved in the Chinese typical culture preservation center on April 30, 2019 with the preservation number being CCTCC No: M 2019320. The embodiment of the invention provides the zearalenone detoxifying agent. The zearalenone detoxifying agent has good specificity for zearalenone, the zearalenone is degraded effectively, at the same time, a degradation reactionis irreversible, in the degradation process, nutrients cannot be adsorbed, and thus the nutrients in contaminated feed are ensured not to be degraded.

The invention discloses a detection probe for leptocylindrus danicus, which belongs to the field of nucleic acid detection. Specifically aiming at a rRNA particular area of leptocylindrus danicus, a set of probes taking a S1 enzyme protection analysis probe as a core is formed, and in combination with S1 enzyme protection analysis and sandwich hybridization technologies, the qualitative and quantitative detection on leptocylindrus danicus rRNA is realized. The detection probe comprises three correlated oligonucleotide probes, namely, a S1 enzyme protection analysis probe, a capture probe, and a signal probe or a connecting probe. The probe disclosed by the invention is good in applicability, and greatly facilitates the finding and implementation of specific probes. The stability of the detection probe disclosed by the invention is far higher than that in the sandwich hybridization technology.

A detecting probe for phycobiont is disclosed. A group of probes, whose core is S1 enzyme protecting and analyzing probe, is synthesized for the particular rRNA region of phycobiont in order to quaitatively and quantitatively detect the rRNA of phycobiont. Said detecting probe includes S1 enzye protecting and analyzing probe, capture probe and signal probe or binding probe.

A testing probe for a coscinodiscus, concretely aiming at the rRNA special area of coscinodiscus, compose a group of testing probe having s1 enzyme protecting analysis probe as the core, combining s1 enzyme protecting analysis with sandwich hybridization technology to realize the quantitative and qualitative testing for rRNA of coscinodiscus. The probe includes three correlative oligonucleotide probe :S1 enzyme protecting analysis probe, catching probe, signal probe or connection probe.