Detection probe for ocean Prorocentrum

Abstract

A detection probe for Prorocentrum micans Ehrenberg belongs to the nucleic acid detection field. It is used for S1 enzyme protection analysis and sandwich hybridization detection of the Prorocentrum micans Ehrenberg, and comprises three correlative oligonucleotide probes. Wherein a S1 enzyme protection analysis probe only serves as a complement of the nucleotides in the Prorocentrum micans Ehrenberg ribosome big subunit 430~520 regions, and shows no homology with other Prorocentrum micans Ehrenberg ribosome RNAs. The S1 enzyme protection analysis probe has a length of 58bp, and can be combined specifically onto the rRNAs of the Prorocentrum micans Ehrenberg under certain conditions, and the sequence is: 5'-ACCAGGCACATTCACCCACCCAGGGGTAGGCTACCATGTCCCTGGAGTTTTCCTCGAT. The invented probes have excellent applicability to make it convenient to seek and realize the specific probe. The invention has higher stability than that of the sandwich hybridization technology.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes used for algae detection in the field of nucleic acid detection, in particular to specific probes for qualitative and quantitative detection of marine Prorocentrum. Background technique [0002] Prorocentrum micans is a common dinoflagellate that is widely distributed in coastal areas, estuaries, oceanic waters, and China's offshore waters, East China Sea, Hong Kong, and Nansha Islands. It is one of the main red tide organisms. Harmful red tides have been formed in the waters of Zhoushan and Hong Kong many times, causing a large number of deaths of farmed fish, posing a huge threat to marine fisheries. Therefore, it is of great significance to detect the population dynamics of marine Prorocentrum in the marine environment in a timely, accurate and rapid manner, and to keep abreast of its quantity changes in the ocean. The traditional identification method of directly observing and counting wit...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, RNA degradation needs to be prevented during the entire detection process, but Various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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