Detection probe of Skeletonema costatum

Abstract

The invention discloses a detection probe for Skeletons costa, which belongs to the field of nucleic acid detection. A set of probes with the S1 enzyme protection analysis probe as the core was synthesized specifically for the specific region of the rRNA of S. costa, combined with the S1 enzyme protection analysis and sandwich hybridization technology to realize the qualitative and quantitative detection of the rRNA of S. costa. The detection probes include three interrelated oligonucleotide probes: S1 enzyme protection analysis probe, capture probe, signal probe or connection probe. The probe of the invention has good applicability and greatly facilitates the search and realization of the specific probe. The stability of the present invention is much higher than that of the sandwich hybridization technique.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for the qualitative and quantitative detection of Skeletalum costatum. It belongs to the field of nucleic acid detection. Background technique [0002] Skeletonema costatum is a kind of eurythermal and euryhaline phytoplankton that widely exists in the world, and it is also one of the most common dominant species of diatoms in my country's coastal waters. This species is a good bait for marine secondary producers, and it is also a common red tide organism in my country's coastal waters and a good pollution indicator. In economical seaweed farming areas, it often competes with kelp and laver for nutrients. Cause economic algae to change color, even rot and lose edible and commercial value; it is also reported that the bloom of Sclerostena mesoscarcinus as the dominant species can cause damage to the gills of Atlantic salmon and eventually die...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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