The invention belongs to the technical field of clean rooms, in particular relates to a clean room with a non-thermal plasmas air disinfection purifier. The clean room comprises an air purification unit, a reactor power, a fan, the clean room, a fresh air system, a controller, an air return trench, an air filter, a static pressure box, a return air pipe and a grating floor. The air purification unit is installed in the static pressure box arranged above the clean room to form an airtight environment; the air purification unit is a plasma reactor, is internally provided with a plurality of nickel-chromium metal wires or metal bands which are arranged in parallel according to equal distance in the same plane to prepare a positive electrode, two ends of the air purification unit are fixed in a corresponding groove or convex part on a conductive rail for preventing micro-discharge, and a switch tube in the reactor power is arranged by a single-end flyback inverter. The invention provides the clean room, which has the advantages of comprehensive air purification and disinfection effect, high efficiency, low energy consumption, low noise, simple structure, maintenance cost saving and long service life and can be applied to an industrial or biological clean room.