38 results about "Skeletonema costatum" patented technology

Subterranean fluids are provided that have improved environmental characteristics, and more particularly, subterranean fluids are provided that include a hydraulic cement in an invert emulsion. Methods of using such fluids in subterranean operations also are provided. An example of a method is a method of using a fluid in a subterranean formation. An example of a composition is a fluid comprising a hydraulic cement and an invert emulsion of an oleaginous fluid, a nonoleaginous fluid, and an emulsifying surfactant, wherein the emulsifying surfactant: has an LC50 or EC50 that is greater than about 10 milligrams / liter with respect to Skeletonema costatum, Acartia tonsa, Scopthalmus maximus (juvenile), or Corophium volutator, and demonstrates a biodegradability of: greater than about 70% biodegradation in 28 days when tested according to method OECD 301A or 301E; or greater than about 60% biodegradation in 28 days when tested according to method OECD 301B, 301C, 301F, or 306.

Subterranean fluids are provided that have improved environmental characteristics, and more particularly, subterranean fluids are provided that include a hydraulic cement in an invert emulsion. Methods of using such fluids in subterranean operations also are provided. An example of a method is a method of using a fluid in a subterranean formation. An example of a composition is a fluid comprising a hydraulic cement and an invert emulsion of an oleaginous fluid, a nonoleaginous fluid, and an emulsifying surfactant, wherein the emulsifying surfactant: has an LC50 or EC50 that is greater than about 10 milligrams / liter with respect to Skeletonema costatum, Acartia tonsa, Scopthalmus maximus (juvenile), or Corophium volutator; and demonstrates a biodegradability of: greater than about 70% biodegradation in 28 days when tested according to method OECD 301A or 301E; or greater than about 60% biodegradation in 28 days when tested according to method OECD 301B, 301C, 301F, or 306.

The invention relates to offspring seed breeding technology of aquaculture, in particular to a fry breeding method for thais luteostoma. Wild adult individuals of the thais luteostoma are collected in an open sea at the temperature of 22-28 DEG C as parent luteostoma, breeding density is 2-5 / m<3>, intensive breeding is conducted, eggs are laid, egg capsules are hatched into swimming larva according to hatching density as 3000-6000 egg capsules / m<3>, the swimming larva is fed with swimming larva chrysophyceae, skeletonema costatum, chlorella or chaetoceros according to feeding quantity of 30-100 thousand / mL, after the larva is turned to be baby luteostoma, feeding quantity is10-20 g per time for 1000 young luteostoma, water is changed and disinfected timely, the baby luteostoma is turned to be young luteostoma after 25-45 days, and the young luteostoma can be cultivated. The fry breeding method for the thais luteostoma is simple to operate, achieves scale breeding of thais luteostoma fry, and improves fry yield and survival rate of the thais luteostoma on the basis of reducing cost.

The invention relates to the technical field of cosmetics and in particular relates to a long-lasting moistening skin conditioning agent and a preparation method and application thereof. The skin conditioning agent is prepared by extracting the following raw materials in percentage by weight: 5-30% of porphyridium, 5-30% of Skeletonema costatum, 5-30% of tremella fuciformis, 3-30% of lentinus edodes, 5-30% of dahurian larch, and 5-30% of ophiopogon root. The conditioning agent has excellent moistening effect, can be used for effectively reducing dandruff, dryness heat and other symptoms caused by skin dryness; in addition, the conditioning agent has good affinity with skin membrane protein, is capable of enhancing the absorption of skin to the nutrients, increasing the hydration function of the cells, and ensuring that the self tolerance of the skin in a dry environment is improved.

The present invention discloses a method to test the growth rate of the skeletonema costatum, which includes the procedures as follows: a. the extraction of RNA of skeletonema costatum, b. separation and cloning of partial sequences of the PCNA gene of the skeletonema costatum, c. cloning of partial sequences of Cytb gene of skeletonema costatum, d. a standard real-time quantitative PCR curve used to test genes of PCNA and Cytb of skeletonema costatum is developed, based on the gene sequences of both PCNA and Cytb, e. the growth rate of the skeletonema costatum is tested based on the expression of quantity (REQ) of gene PCNA of skeletonema costatum, with PCNA as the target gene and the Cytb as the housekeeping gene. The present invention provides a technical method for accurate estimation on the growth rate on the spot of the skeletonema costatum, which can be used to estimate the productivity of the floating algae, study the variance of the alga species, construct the zoological dynamic model and even forecast the red tide.

The present invention provides a genetic marker and method for quickly quantitatively detecting skeletonema costatum. It provides skeletonema costatum ribosomal DNA (rDNA) and transcription unit internal spacer (ITS) nucleotide sequence, and oligonucleotide probe designed by using said sequence as basis. The invention utilizes the sequence comparison and experiment to show that these probes are genetic marker peculiary to the skeletonema costatum. The invention also provides the method for quickly quantitatively detecting skeletonema costatum.

The invention discloses a marine functional facial mask for refining pores. The facial mask is prepared from the following components in parts by weight: 1-8 parts of alfonsino collagen peptide, 1-4 parts of skeletonema costatum algae extract, 30-45 parts of deep sea water, 2-4 parts of crithmum maritimum extract, 1-5 parts of black pearl powder, 10-20 parts of oceanic sediment, 2-6 parts of avocado oil and 1-5 parts of vitamin C. The facial mask is prepared from purely natural ingredients, can sufficiently achieve a synergistic effect of marine active ingredients and plant extract, has a good pore shrinking effect, and does not have side effect or dependent effect.

The invention discloses moisturizing composition containing snow lotus extract and a skin care product. The moisturizing composition has good moisturizing and water-blocking ability and comprises, byweight, 4-8 parts of skeletonema costatum extract, 2-9 parts of Quinoa seed extract, 2-9 parts of hypnea musciformis extract, 10-20 parts of snow lotus extract and 3-7 parts of centella asiatica extract. The moisturizing composition containing snow lotus extract belongs to the technical field of skin care products.

The invention provides a cultivation method for improving disease resistance and stress resistance of epinephelus spp. fries. The cultivation method comprises the following steps of: 1) in two days before fries are added, introducing a variety of skeletonema costatum into a nursery pond; 2) within seven days after fries are added, adding SS type rotifers in each day; 3) during the 8th-15th day offry adding, adding fairy shrimp nauplius and LS type rotifers in each day; 4) during the 16th-25th days of the fry adding, adding the fairy shrimp nauplius in each day; and 5) during the 26th-35th days of the fry adding, adding the fairy shrimp nauplius and compound feed in each day. The nutrients of fingerlings during different periods can be regulated, fry immunity is improved, and the disease resistance and stress resistance of the epinephelus spp. fries can be improved. Through experiments, under a condition that salinity is 3.6%, a survival rate still can be above 68%, a survival rate still can be kept at about 30% under a condition that the salinity is 4.2%, and the survival rate is kept to be 70% or above after the epinephelus spp. fries are infected with vibrio alginnolyficus for two weeks.

The invention discloses novel application of salicornia europaea and a root extractive thereof. The salicornia europae (Salicornia europaea L.) and the root extractive thereof provided by the invention are used for inhibiting the algae growth causing red tide and inhibiting the red tide. The algae causing the red tide is selected from at least one of skeletonema costatum, prorocentrum donghaiense and prorocentrum minimum. The method for preparing the root extractive of the salicornia europae comprises the following steps: using an organic solvent as an extractant, and extracting from the root of the salicornia europaea, wherein the organic solvent is selected from any one of petroleum ether, chloroform, methanol and normal butanol. The result of the test shows that four different root extractives of the salicornia europaea can strongly inhibit the growth and the photosynthesis of three kinds of algae; for different algae, the allelopathy strength is different; for the skeletonema costatum, the allelopathy of the petroleum ether phase extractive is the strongest, and IC50 is 1.633 mg / L; for the prorocentrum donghaiense, the function of the chloroform phase extractive is the strongest, and IC50 is 13. 799 mg / L; and for the prorocentrum minimum, the allelopathy is improved along with the increasing of the polarity of the extractive, the function of the methanol phase is the strongest, and IC50 is 1.138 mg / L.

The invention belongs to the water processing field, especially relating to a method for preparing an Skeletonema costatuma inhibitor, a method for preparing Ulva linza Linnaeus extract for inhibiting Skeletonema costatuma: leaching Ulva linza Linnaeus with sterilized sea water, alcohol, acetone, and aether or chloroform, concentrating the obtained extract by rotary evaporation, putting the concentrated extract into a to-be-processed water body to inhibit red tide alga growth in the water body, where ordinarily, per kg Ulva linza Linnaeus is leached with 30-50 L sea water for 0.5-2 days, the obtained extract is concentrated to 10% of its original bulk, and 2-10 ml concentrate is putted into per L to-be- processed water body. And the invention fully uses Ulva linza Linnaeus in Ulva linza Linnaeus plants, effectively inhibiting growth of harmful marine red tide alga, especially Skeletonema costatuma, and can be widely applied to various water bodies to implement red tide.

The invention discloses halobacillus kuroshimensis HSQAY1 which is collected in the China center for type culture collection on 12, June 2012 with the collection number of CCTCC NO: M2012221. The invention also discloses application of the strain in skeletonema costatum in the degradable red tide outbreak sea region. According to the strain, skeletonema costatum cells arranged in a chain shape can be broken, so that the cells are expanded into a ball shape and are finally broken to die; and moreover, the strain is autochthonous bacteria and is expected to exert important functions in biological treatment practice in red tide, and secondary pollution or alien invasive species and other pollution conditions are avoided in actual application.

A method for improving a survival rate of charybdis japonica larvae comprises the steps of disinfection of ovigerous crabs, collection of larvae, disinfection of live food and combination of various baits and the like. The method of performing combined feeding of skeletonema costatum, chlorella, live rotifers and artemia nauplii not only can regulate water quality and makes the water quality not deteriorate easily, but also meets nutrition requirements of the charybdis japonica larvae, the charybdis japonica larvae smoothly casts off a skin and performs metamorphosis development, and thus the survival rate of the larvae is improved. The ovigerous crabs and the rotifers and artemia nauplii as the live baits are disinfected, filamentous bacterium disease pathogens are prevented from being brought into a culture tank, and the outbreak of the filamentous bacterium disease in the process of cultivating the charybdis japonica larvae is effectively prevented. According to characteristics of development of the charybdis japonica larvae, the method is scientific and reasonable, the survival rate of the charybdis japonica larvae is improved, and rapid development of the charybdis japonica breeding industry is promoted.

The present invention discloses a RFQ-PCR detection method of skeletonema costatum cytochrome b gene. Said method includes the following steps: (a), extracting skeletonema costatum RNA as standard sample; (b), separating and cloning skeletonema costatum cytochrome b gene to obtain skeletonema costatum cytochrome b gene sequence, and obtaining cytochrome b sequences of several algal seeds which are approaching to the skeletonema costatum in status from GenBank, and constructing system tree; (c), making RFQ-PCR; and (d) utilizing obtained skeletonema costatum cytochrome b gene sequence with 700-740bp to construct standard curve and utilizing said standard curve to detect the skeletonema costatum cytochrome b gene to be detected.

The invention discloses an aryl thiazole-tryptamine compound for killing skeletonema costatum and a combination agent thereof. The combination agent for killing the skeletonema costatum is composed of aryl thiazole-tryptamine, isothiazolinone, an auxiliary ingredient and a solvent. The combination agent has the advantages of being simple in synthesis / preparation, remarkable in effect, lasting in drug effect, high in biological safety and the like, growth of skeletonema costatum can be inhibited for a long time while the biological safety is guaranteed, and after 10 days, growth of skeletonema costatum still does not rebound. The compound and the combination agent thereof are suitable for industrial production and popularization.

The invention discloses an enzyme-linked immuno sorbent assay (ELISA) detection method of Skeletonema costatum. The method comprises the steps of: (a) repeatedly picking individual algal cell to obtain pure culture under a microscope; (b) preparing an ELISA plate; (c) preparing and diluting test reagents: uniformly mixing concentrated washing liquid (if there are crystals, fully dissolve and mix before usage), and diluting the concentrated washing liquid with deionized water (or distilled water) according to the proportion of 1:1, and storing at 4 DEG C for standby; (d) using a double antibody sandwich ELISA method to complete an antibody test, adding a confining liquid and conducting enclosing treatment on removed micropore strips; and (e) detecting the OD value of an enzyme-labeled second antibody participated in the reaction after coloration by using a microplate reader at single wavelength of 450 nm or dual wavelength of 450 nm / 630 nm (use a blank aperture for zeroing when single wavelength determination is employed), recording the results and calculating the concentration. The detection method provided by the invention has the advantages of specificity, sensitivity, high efficiency, easy operation and low cost, and can analyze a large amount of samples at the same time; therefore, the method is an effective means for qualitative and quantitative analysis of target antigens.

The invention provides a Skeletonema costatum culture medium formula. A specific technical scheme is as follows: a Skeletonema costatum culture medium formula is as follows: 0.4g of KNO3, 4mg of KH2PO4, 0.5g of NaHCO3, 5 IU of a 920 growth hormone (namely gibberellin), 1 ml of an f / 2 trace element solution, 1 ml of an f / 2 vitamin solution and 1000 ml of seawater. The f / 2 trace element solution comprises 23 mg of ZnSO4.4H2O, 178 mg of MnCl2.4H2O, 10 mg of CuSO4.5H2O, 3.9 g of FeC6H5O7.5H2O, 7.3 mg of Na2MoO4.2H2O, 12 mg of CoCl2.6H2O, 4.35 g of Na2EDTA, and 1000 ml of pure water; and the f / 2 vitamin solution comprises 0.5 mg of vitamin B12, 100 mg of vitamin B1, 0.5 mg of vitamin H and 1000 ml of pure water. The optimized medium and a plastic film suspension sling method for culturing Skeletonema costatum have advantages of fast growth, short production cycle, high yield, high content of highly unsaturated fatty acid, low cost, easy operation, no pollution, easy control, flexibility, safety and reliability.

The invention discloses an alga inhibition application of a cyclodipeptide compound, wherein the inhibited alga herein is a red tide microalga selected from: Amphidinium carterae, Heterosigma akashiwo, Phaeocystis globsa, Prorocentrum donghaiense, or Skeletonema costatum. The cyclodipeptide compound is pyrrolopiperazine-2,5-dione or 3-hydromethyl-pyrrolopiperazine-2,5-dione. The invention also discloses a measurement method of the alga inhibition activity of the cyclodipeptide compound. The cyclodipeptide compound has good alga inhibition effect, and the application field of the compound is broadened.

A detecting probe for mid-rib-bar seaweed is disclosed. A group of probes, whose core is SI enzyme protecting and analyzing probe, is synthesized for the particular rRNA region of mid-rib-bar seaweed in order to qualitatively and quantitatively detect the rRNA of said seaweed. Said tetecting probe includes Si enzyme protecting and analyzing probe, capture probe and signal probe or binding probe.

The invention provides a method for preparing phenolic acid and alkaloid by separation and purification from ulva pertusa kjellm. The method comprises the following steps: adding ulva pertusa kjellm dry powder into absolute methanol to prepare a steamed object, and adding distilled water to prepare supernate; regulating pH of the supernate, and adding ethyl acetate to extract for multiple times to obtain components A, B, C and D; spotting methanol solution to silica gel GF254, combining and collecting strips at same Rf positions, dissolving in acetone, preparing eleven components by centrifuge and concentration in reduced pressure, and spotting four of the components to the silica gel GF254 by a streaking method once again for purifying preparation to obtain six components, wherein thirteen components are phenolic acid and alkaloid, and can be used for inhibiting growth of karenia mikimotoi or skeletonema costatum. The method is strong in operability, the prepared phenolic acid and alkaloid are high in purity, and the breakthrough of phenolic acid and alkaloid prepared by extracting ulva pertusa kjellm can be realized. The phenolic acid and alkaloid prepared by the method have algal inhibition activity, and have practical application values.

The invention discloses a skeletonema costatum lytic virus and its separation method and use. The skeletonema costatum lytic virus is a skeletonema costatum single chain DNA lytic virus ScssDNAV, is preserved in the China general microbiological culture collection center (CGMCC) Oon May 25, 2015, has a preservation number of CGMCC No. 10599, is used for inhibiting skeletonema costatum growth and killing skeletonema costatum, has strong pyrolysis effects on skeletonema costatum NMBguh004-1 and NMBguh004-2, realizes safe, effective, fast and specific degradation of skeletonema costatum in sea water and has an algae chlorophyll a concentration reduction rate of 76.14%.

The invention discloses a method for breeding calanus finmarchicus with a procelin jar and belongs to the technical field of aquaculture. In the prior art, an outdoor extensive mode and an indoor intensive mode are used for breeding calanus finmarchicus; an outdoor earth pond mode is low in breeding cost but low in yield, the outdoor earth pond mode is limited by the seasons and water temperature, and the calanus finmarchicus is prone to pollution of viruses and bacteria; the intensive breeding mode is high in yield and scarcely influenced by natural conditions, but the cost is high. Compared with the prior art, the method has the advantages that the porcelain jar is firm, durable, not prone to breakage, convenient to clean and capable of being repeatedly used, operation is convenient, and investment is saved; the calanus finmarchicus is fed with germs, cyclotella, skeletonema costatum and rhombus thalassiosira, the calanus finmarchicus grows rapidly, propagates rapidly and is robust and high in yield, and the limitation of the seasons is avoided.

The invention relates to the technical field of cosmetics and in particular relates to a long-lasting moistening skin conditioning agent and a preparation method and application thereof. The skin conditioning agent is prepared by extracting the following raw materials in percentage by weight: 5-30% of porphyridium, 5-30% of Skeletonema costatum, 5-30% of tremella fuciformis, 3-30% of lentinus edodes, 5-30% of dahurian larch, and 5-30% of ophiopogon root. The conditioning agent has excellent moistening effect, can be used for effectively reducing dandruff, dryness heat and other symptoms caused by skin dryness; in addition, the conditioning agent has good affinity with skin membrane protein, is capable of enhancing the absorption of skin to the nutrients, increasing the hydration function of the cells, and ensuring that the self tolerance of the skin in a dry environment is improved.

A method for improving a survival rate of charybdis japonica larvae comprises the steps of disinfection of ovigerous crabs, collection of larvae, disinfection of live food and combination of various baits and the like. The method of performing combined feeding of skeletonema costatum, chlorella, live rotifers and artemia nauplii not only can regulate water quality and makes the water quality not deteriorate easily, but also meets nutrition requirements of the charybdis japonica larvae, the charybdis japonica larvae smoothly casts off a skin and performs metamorphosis development, and thus the survival rate of the larvae is improved. The ovigerous crabs and the rotifers and artemia nauplii as the live baits are disinfected, filamentous bacterium disease pathogens are prevented from being brought into a culture tank, and the outbreak of the filamentous bacterium disease in the process of cultivating the charybdis japonica larvae is effectively prevented. According to characteristics of development of the charybdis japonica larvae, the method is scientific and reasonable, the survival rate of the charybdis japonica larvae is improved, and rapid development of the charybdis japonica breeding industry is promoted.

The invention discloses a skeletonema costatum lytic virus and its separation method and use. The skeletonema costatum lytic virus is a skeletonema costatum single chain DNA lytic virus ScssDNAV, is preserved in the China general microbiological culture collection center (CGMCC) Oon May 25, 2015, has a preservation number of CGMCC No. 10599, is used for inhibiting skeletonema costatum growth and killing skeletonema costatum, has strong pyrolysis effects on skeletonema costatum NMBguh004-1 and NMBguh004-2, realizes safe, effective, fast and specific degradation of skeletonema costatum in sea water and has an algae chlorophyll a concentration reduction rate of 76.14%.

The invention belongs to the water processing field, especially relating to a method for preparing an Skeletonema costatuma inhibitor, a method for preparing Ulva linza Linnaeus extract for inhibiting Skeletonema costatuma: leaching Ulva linza Linnaeus with sterilized sea water, alcohol, acetone, and aether or chloroform, concentrating the obtained extract by rotary evaporation, putting the concentrated extract into a to-be-processed water body to inhibit red tide alga growth in the water body, where ordinarily, per kg Ulva linza Linnaeus is leached with 30-50 L sea water for 0.5-2 days, the obtained extract is concentrated to 10% of its original bulk, and 2-10 ml concentrate is putted into per L to-be- processed water body. And the invention fully uses Ulva linza Linnaeus in Ulva linza Linnaeus plants, effectively inhibiting growth of harmful marine red tide alga, especially Skeletonema costatuma, and can be widely applied to various water bodies to implement red tide.

The invention discloses an alga inhibition application of a cyclodipeptide compound, wherein the inhibited alga herein is a red tide microalga selected from: Amphidinium carterae, Heterosigma akashiwo, Phaeocystis globsa, Prorocentrum donghaiense, or Skeletonema costatum. The cyclodipeptide compound is pyrrolopiperazine-2,5-dione or 3-hydromethyl-pyrrolopiperazine-2,5-dione. The invention also discloses a measurement method of the alga inhibition activity of the cyclodipeptide compound. The cyclodipeptide compound has good alga inhibition effect, and the application field of the compound is broadened.

The invention provides a Skeletonema costatum culture medium formula. A specific technical scheme is as follows: a Skeletonema costatum culture medium formula is as follows: 0.4g of KNO3, 4mg of KH2PO4, 0.5g of NaHCO3, 5 IU of a 920 growth hormone (namely gibberellin), 1 ml of an f / 2 trace element solution, 1 ml of an f / 2 vitamin solution and 1000 ml of seawater. The f / 2 trace element solution comprises 23 mg of ZnSO4.4H2O, 178 mg of MnCl2.4H2O, 10 mg of CuSO4.5H2O, 3.9 g of FeC6H5O7.5H2O, 7.3 mg of Na2MoO4.2H2O, 12 mg of CoCl2.6H2O, 4.35 g of Na2EDTA, and 1000 ml of pure water; and the f / 2 vitamin solution comprises 0.5 mg of vitamin B12, 100 mg of vitamin B1, 0.5 mg of vitamin H and 1000 ml of pure water. The optimized medium and a plastic film suspension sling method for culturing Skeletonema costatum have advantages of fast growth, short production cycle, high yield, high content of highly unsaturated fatty acid, low cost, easy operation, no pollution, easy control, flexibility, safety and reliability.