38 results about "Skeletonema costatum" patented technology

Subterranean fluids are provided that have improved environmental characteristics, and more particularly, subterranean fluids are provided that include a hydraulic cement in an invert emulsion. Methods of using such fluids in subterranean operations also are provided. An example of a method is a method of using a fluid in a subterranean formation. An example of a composition is a fluid comprising a hydraulic cement and an invert emulsion of an oleaginous fluid, a nonoleaginous fluid, and an emulsifying surfactant, wherein the emulsifying surfactant: has an LC50 or EC50 that is greater than about 10 milligrams / liter with respect to Skeletonema costatum, Acartia tonsa, Scopthalmus maximus (juvenile), or Corophium volutator; and demonstrates a biodegradability of: greater than about 70% biodegradation in 28 days when tested according to method OECD 301A or 301E; or greater than about 60% biodegradation in 28 days when tested according to method OECD 301B, 301C, 301F, or 306.

The present invention discloses a skeletonema costatum PCNA gene detection method. Said method includes the following steps: (1), extracting RNA of skeletonema costatum; (2), separating and cloning partial sequence of skeletonema costatum PCNA gene, utilizing 3'RACE to make the obtained skeletonema costatum PCNA sequence implement 3' end extension so as to obtain PCNA sequence with 750-850bp; and (3), according to the obtained PCNA sequence with 750-850bp constructing standard curve and utilizing said standard curve to detect the skeletonema costatum PCNA gene to be detected. According the method of the invention, through the skeletonema costatum PCNA gene, the productivity of the floating alga can be detected and estimated, the vicissitude of the floating alga cluster can be investigated, the zoology dynamic model can be established and even some features can be predicted. Therefore, the defect that the locale zoology parameters of a single floating alga can not be detected in prior art is effectively overcome.

The invention relates to the technical field of cosmetics and in particular relates to a long-lasting moistening skin conditioning agent and a preparation method and application thereof. The skin conditioning agent is prepared by extracting the following raw materials in percentage by weight: 5-30% of porphyridium, 5-30% of Skeletonema costatum, 5-30% of tremella fuciformis, 3-30% of lentinus edodes, 5-30% of dahurian larch, and 5-30% of ophiopogon root. The conditioning agent has excellent moistening effect, can be used for effectively reducing dandruff, dryness heat and other symptoms caused by skin dryness; in addition, the conditioning agent has good affinity with skin membrane protein, is capable of enhancing the absorption of skin to the nutrients, increasing the hydration function of the cells, and ensuring that the self tolerance of the skin in a dry environment is improved.

A method for improving a survival rate of charybdis japonica larvae comprises the steps of disinfection of ovigerous crabs, collection of larvae, disinfection of live food and combination of various baits and the like. The method of performing combined feeding of skeletonema costatum, chlorella, live rotifers and artemia nauplii not only can regulate water quality and makes the water quality not deteriorate easily, but also meets nutrition requirements of the charybdis japonica larvae, the charybdis japonica larvae smoothly casts off a skin and performs metamorphosis development, and thus the survival rate of the larvae is improved. The ovigerous crabs and the rotifers and artemia nauplii as the live baits are disinfected, filamentous bacterium disease pathogens are prevented from being brought into a culture tank, and the outbreak of the filamentous bacterium disease in the process of cultivating the charybdis japonica larvae is effectively prevented. According to characteristics of development of the charybdis japonica larvae, the method is scientific and reasonable, the survival rate of the charybdis japonica larvae is improved, and rapid development of the charybdis japonica breeding industry is promoted.

The invention discloses a skeletonema costatum lytic virus and its separation method and use. The skeletonema costatum lytic virus is a skeletonema costatum single chain DNA lytic virus ScssDNAV, is preserved in the China general microbiological culture collection center (CGMCC) Oon May 25, 2015, has a preservation number of CGMCC No. 10599, is used for inhibiting skeletonema costatum growth and killing skeletonema costatum, has strong pyrolysis effects on skeletonema costatum NMBguh004-1 and NMBguh004-2, realizes safe, effective, fast and specific degradation of skeletonema costatum in sea water and has an algae chlorophyll a concentration reduction rate of 76.14%.

The invention belongs to the water processing field, especially relating to a method for preparing an Skeletonema costatuma inhibitor, a method for preparing Ulva linza Linnaeus extract for inhibiting Skeletonema costatuma: leaching Ulva linza Linnaeus with sterilized sea water, alcohol, acetone, and aether or chloroform, concentrating the obtained extract by rotary evaporation, putting the concentrated extract into a to-be-processed water body to inhibit red tide alga growth in the water body, where ordinarily, per kg Ulva linza Linnaeus is leached with 30-50 L sea water for 0.5-2 days, the obtained extract is concentrated to 10% of its original bulk, and 2-10 ml concentrate is putted into per L to-be- processed water body. And the invention fully uses Ulva linza Linnaeus in Ulva linza Linnaeus plants, effectively inhibiting growth of harmful marine red tide alga, especially Skeletonema costatuma, and can be widely applied to various water bodies to implement red tide.

The invention provides a Skeletonema costatum culture medium formula. A specific technical scheme is as follows: a Skeletonema costatum culture medium formula is as follows: 0.4g of KNO3, 4mg of KH2PO4, 0.5g of NaHCO3, 5 IU of a 920 growth hormone (namely gibberellin), 1 ml of an f / 2 trace element solution, 1 ml of an f / 2 vitamin solution and 1000 ml of seawater. The f / 2 trace element solution comprises 23 mg of ZnSO4.4H2O, 178 mg of MnCl2.4H2O, 10 mg of CuSO4.5H2O, 3.9 g of FeC6H5O7.5H2O, 7.3 mg of Na2MoO4.2H2O, 12 mg of CoCl2.6H2O, 4.35 g of Na2EDTA, and 1000 ml of pure water; and the f / 2 vitamin solution comprises 0.5 mg of vitamin B12, 100 mg of vitamin B1, 0.5 mg of vitamin H and 1000 ml of pure water. The optimized medium and a plastic film suspension sling method for culturing Skeletonema costatum have advantages of fast growth, short production cycle, high yield, high content of highly unsaturated fatty acid, low cost, easy operation, no pollution, easy control, flexibility, safety and reliability.