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Algae-inhibiting use of cyclic dipeptide compounds and assay method for algae-inhibiting activity

A technology of compound and cyclic dipeptide, which is applied in the field of algae inhibitory activity of cyclic dipeptide compounds on red tide microalgae, can solve the problems of no formation, insufficient comprehensive and perfect biological activity of cyclic dipeptide, and achieve convenient operation and algae inhibition Good effect, reasonable design effect

Active Publication Date: 2019-09-06
江苏一青生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the biological activity of cyclic dipeptides is not comprehensive and perfect enough, and a complete system has not been formed. Gracilaria lemaneiformis ) to 3-hydroxymethyl-pyrrolopiperazine-2,5-dione
However, unfortunately, in the research on the activity of cyclic dipeptide compounds, there is no research on its anti-algae activity at home and abroad.

Method used

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  • Algae-inhibiting use of cyclic dipeptide compounds and assay method for algae-inhibiting activity
  • Algae-inhibiting use of cyclic dipeptide compounds and assay method for algae-inhibiting activity
  • Algae-inhibiting use of cyclic dipeptide compounds and assay method for algae-inhibiting activity

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Embodiment 1

[0021] Embodiment 1, the assay method of the anti-algae activity of cyclic dipeptide compounds:

[0022] Cyclic dipeptide compounds pyrrolopiperazine-2, 5-dione and 3-hydroxymethyl-pyrrolopiperazine-2, 5-dione were respectively dissolved in an appropriate amount of methanol (analytical grade) to a concentration of 5 g / L mother liquor. Pipette different volumes of mother liquor and add them to the red tide microalgae culture solution in the exponential growth phase, so that the final concentrations of cyclic dipeptides are 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL. mL (the maximum pipetting volume shall not exceed 1% of the culture system volume). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, potassium dichromate was set as the positive control group, that is, different volumes of potassium dichromate aqueous s...

Embodiment 2

[0024] Embodiment 2, the assay experiment one of the anti-algae activity of cyclic dipeptide compounds, with reference to Figure 1-3 :

[0025](1) Dissolve 0.01 g of cyclic dipeptide compounds in 2 mL of methanol (analytical grade) to prepare a stock solution with a concentration of 5 g / L; (2) Pipette 1.6 µL, 8.0 µL, 40 µL and 200 µL of the stock solution, Add it to the red tide microalgae culture solution in the exponential growth phase, and the final volume of the culture system is 20 mL. The final concentrations of cyclic dipeptides were 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL (the maximum pipetting volume was 1% of the final volume of the culture system). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, 0.025 g of potassium dichromate (analytical grade) was dissolved in 5 mL of distilled water at a concent...

Embodiment 3

[0029] Embodiment 3, Determination experiment two of the anti-algae activity of cyclic dipeptide compounds:

[0030] (1) Dissolve 0.02 g of cyclic dipeptide compounds in 4 mL of methanol (analytical grade) to prepare a stock solution with a concentration of 5 g / L; (2) Pipette 8 µL, 40 µL, 200 µL and 1000 µL of the stock solution, Add it to the red tide microalgae culture solution in the exponential growth phase, and the final volume of the culture system is 100 mL. The final concentrations of cyclic dipeptides were 0.4 µg / mL, 2 µg / mL, 10 µg / mL and 50 µg / mL (the maximum pipetting volume was 1% of the final volume of the culture system). At the same time, the same volume of methanol was pipetted into the red tide microalgae culture solution as a control group to eliminate the influence of methanol on the growth of red tide microalgae. In addition, 0.05 g of potassium dichromate (analytical grade) was dissolved in 10 mL of distilled water at a concentration of 5 g / L. Pipette 10...

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Abstract

The invention discloses an alga inhibition application of a cyclodipeptide compound, wherein the inhibited alga herein is a red tide microalga selected from: Amphidinium carterae, Heterosigma akashiwo, Phaeocystis globsa, Prorocentrum donghaiense, or Skeletonema costatum. The cyclodipeptide compound is pyrrolopiperazine-2,5-dione or 3-hydromethyl-pyrrolopiperazine-2,5-dione. The invention also discloses a measurement method of the alga inhibition activity of the cyclodipeptide compound. The cyclodipeptide compound has good alga inhibition effect, and the application field of the compound is broadened.

Description

technical field [0001] The invention belongs to the field of marine biochemical engineering, and in particular relates to the algae-inhibiting activity of cyclic dipeptide compounds on red tide microalgae. Background technique [0002] Cyclic dipeptides, also known as diketopiperazines (DKPs), are formed by the condensation of two amino acids, and their skeletons have a stable six-membered ring structure. Studies have found that cyclic dipeptide compounds widely exist in nature, and active cyclic dipeptide compounds can be found in animals and plants, yeast, protozoa, fungi and marine organisms. Cyclic dipeptide compounds have physiological activities such as antibacterial, antifouling, antiviral, antitumor, antioxidative, hypoglycemic and intercellular signal mediation, and can also be used as plant growth regulators, immunosuppressants and immune enhancers. In recent years, cyclic dipeptides have attracted great interest of researchers due to their molecular diversity and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P13/00G01N33/00
Inventor 孙颖颖郭赣林位义迎浦寅芳苏振霞
Owner 江苏一青生物科技有限公司
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