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Cultivation method for improving disease resistance and stress resistance of epinephelus spp. fries

A method for cultivating grouper and a technology for grouper, applied in the field of fish farming, can solve problems such as grouper death, grouper stress response, etc., and achieve the effects of improving immunity, realizing effective utilization, and improving stress resistance.

Active Publication Date: 2020-05-22
HAINAN CHENHAI AQUATIC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the grouper cultivation process, after a certain period of time, the fry need to be transferred to outdoor ecological ponds for cultivation. During this process, the environment changes greatly, and the stress response of the grouper often occurs, which leads to a large number of grouper deaths in severe cases. Therefore, It is also extremely important to explore a fish fry breeding method with strong stress resistance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A breeding method for improving disease resistance and stress resistance of grouper fry, comprising the following steps:

[0025] 1) Water quality requirements: pH7.8~8.5, ammonia nitrogen below 0.5mg / g, salinity 28~31‰, water temperature 25~28℃, dissolved oxygen in water greater than 5mg / L;

[0026] 2) Put the seedlings: put the size 25m 3 , the breeding pool with a depth of 1.2 meters is cleaned and disinfected and put into the newly hatched tiger spot larvae, and the stocking density is 30,000 fish / m 3 ;

[0027] 2 days before the seedlings were released, Sclerostenae was introduced into the nursery pond, and the inoculation concentration of Sclerotinia was 4×10 3 a / L;

[0028] Within 7 days after the seedlings are released, feed SS-type rotifers every day. The length of the rotifers is less than 120um and the width is less than 60um. Keep the density of rotifers in the water at 10,000 / L;

[0029] On the 8th to 15th day of seedling release, feed Artemia nauplii a...

Embodiment 2

[0040] A breeding method for improving disease resistance and stress resistance of grouper fry, comprising the following steps:

[0041] 1) Water quality requirements: pH7.8~8.5, ammonia nitrogen below 0.5mg / g, salinity 28~31‰, water temperature 25~28℃, dissolved oxygen in water greater than 5mg / L;

[0042] 2) Put the seedlings: put the size 25m 3 , the breeding pool with a depth of 1.2 meters is cleaned and disinfected and put into the newly hatched tiger spot larvae, and the stocking density is 30,000 fish / m 3 ;

[0043] 2 days before the seedlings were released, Sclerostenae was introduced into the nursery pond, and the inoculation concentration of Sclerotinia was 3×10 3 a / L;

[0044] Within 7 days after the seedlings are released, feed SS-type rotifers every day. The length of the rotifers is less than 120um and the width is less than 60um, and the density of rotifers in the water is kept at 20,000 / L;

[0045] On the 8th to 15th day of seedling release, feed Artemia na...

Embodiment 3

[0056] The difference between this embodiment and embodiment 1 is:

[0057] The rotifers are cultivated in the following way: the rotifers are inoculated in seawater at an initial density of 5 / mL; the salinity of the seawater is 28‰, the pH is 7.8, the dissolved oxygen is greater than 5mg / L, and the water temperature is 26-28°C; Contains a density of 6×10 7 Cell / mL Chlorella, 10mg / L Bacillus subtilis and Lactobacillus rhamnosus fermentation powder, 2mg / L glutamic acid and 1mg / L methionine, cultivated for 8h.

[0058] Artemia nauplii are cultured in the following way: introduce Artemia nauplii into seawater at an initial density of 5 / mL; the salinity of the seawater is 28‰, the pH is 7.8, the dissolved oxygen is greater than 5mg / L, and the water temperature is 26 ~28°C; seawater also contains 10mg / L Bacillus subtilis and Lactobacillus rhamnosus fermentation powder, 20mg / L L-arginine and 4.0×10 7 cell / mL Chlorella, cultured for 4 hours.

[0059] Described fermentation powder:...

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PUM

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Abstract

The invention provides a cultivation method for improving disease resistance and stress resistance of epinephelus spp. fries. The cultivation method comprises the following steps of: 1) in two days before fries are added, introducing a variety of skeletonema costatum into a nursery pond; 2) within seven days after fries are added, adding SS type rotifers in each day; 3) during the 8th-15th day offry adding, adding fairy shrimp nauplius and LS type rotifers in each day; 4) during the 16th-25th days of the fry adding, adding the fairy shrimp nauplius in each day; and 5) during the 26th-35th days of the fry adding, adding the fairy shrimp nauplius and compound feed in each day. The nutrients of fingerlings during different periods can be regulated, fry immunity is improved, and the disease resistance and stress resistance of the epinephelus spp. fries can be improved. Through experiments, under a condition that salinity is 3.6%, a survival rate still can be above 68%, a survival rate still can be kept at about 30% under a condition that the salinity is 4.2%, and the survival rate is kept to be 70% or above after the epinephelus spp. fries are infected with vibrio alginnolyficus for two weeks.

Description

technical field [0001] The invention relates to the technical field of fish breeding, in particular to a cultivation method for improving disease resistance and stress resistance of grouper fry. Background technique [0002] In the grouper breeding process, common diseases include vibriosis, enteritis disease, ringworm disease and cryptocytoniasis, among which bacterial diseases such as vibriosis are one of the main diseases of grouper, mainly caused by algae caused by Vibrio. Common prevention and treatment methods for vibriosis are sprinkling Galla galla or feed with three yellow powder, etc. At present, it is mainly in the form of treatment after the disease, and there are few methods that can effectively improve the disease resistance of fry through the cultivation of fry. [0003] During the grouper cultivation process, after a certain period of time, the fry need to be transferred to outdoor ecological ponds for cultivation. During this process, the environment change...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/13A23K50/80A23K10/20A23K10/22A23K10/12A23K10/30A23K20/20A23K20/26
CPCA01K61/10A01K61/13A23K50/80A23K10/20A23K10/22A23K10/12A23K10/30A23K20/20A23K20/26Y02A40/81Y02A40/818
Inventor 张国庆蔡春有陈艺斌蔡有森蔡建顺黄春仁李伟蔡四川蔡金泉蔡惠明蔡智怀王景宝罗磊蔡艺新陈猛猛李伟峰刘赐福吴光灿
Owner HAINAN CHENHAI AQUATIC CO LTD
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