Method for preparing inhibitor of skeletonema costatum (greville) cleve

A technology of inhibitor and stripe algae, applied in the field of water treatment, can solve the problems of loss of aquaculture, health hazards of aquatic animals and human beings, reduction of dissolved oxygen in seawater, etc. Effect

Inactive Publication Date: 2006-10-25
OCEAN UNIV OF CHINA
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Problems solved by technology

Eutrophication leads to the explosive growth of red tide algae, which will reduce the dissolved oxygen in seawater and bring h...

Method used

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Examples

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Effect test

Embodiment 1

[0014] Soak 0.1 kg of Ulva ulva in 3 liters of sterilized seawater for 1 day, then filter, and concentrate the filtrate to 300 ml. Take concentrated solution 1, 2, 3, 4, 5 milliliters and add respectively in 200 milliliters of Skeletalum costa culture medium, after 5 days, inhibition rate is respectively 65%, 86%, 96%, 987%, 99.5%.

Embodiment 2

[0016] 1 kilogram of Ulva ulva was extracted with 2 liters of ethanol for 12 hours and then filtered, the filtrate was concentrated to 200 milliliters, and 0.5 milliliters of the obtained extract was applied to 200 milliliters of Scutellaria costarum culture medium. After 5 days, the density of the algae was only 3 times that of the control group. %, that is, the inhibition rate is 97%.

[0017] 1 kg of Ulva ulva was extracted with 2 liters of ethanol for 48 hours and then filtered, the filtrate was concentrated to 200 ml, and 0.5 ml of the obtained extract was applied to 200 ml of culture medium of Skeletalum costa, and after 5 days, the density of algae was only 2% of that of the control group , that is, the inhibition rate was 98%.

Embodiment 3

[0019] 1 kg of Ulva ulva was extracted with 2 liters of acetone for 12 hours and then filtered, the filtrate was concentrated to 200 ml, and 0.5 ml of the obtained extract was added to 200 ml of culture medium of Skeletalum costa, and after 5 days the density of the algae was only 3% of that of the control group , that is, the inhibition rate was 97%.

[0020] 1 kg of Ulva ulva was extracted with 2 liters of acetone for 24 hours and then filtered, the filtrate was concentrated to 200 ml, and 0.5 ml of the obtained extract was applied to 200 ml of culture medium of Skeletalum costa. After 5 days, the density of algae was only 2% of that of the control group , that is, the inhibition rate was 98%.

[0021] 1 kg of Ulva ulva was extracted with 2 liters of acetone for 48 hours and then filtered, the filtrate was concentrated to 200 ml, and 0.5 ml of the obtained extract was applied to 200 ml of culture medium of Skeletalum costa, and after 5 days, the density of algae was only 1.5...

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Abstract

The invention belongs to the water processing field, especially relating to a method for preparing an Skeletonema costatuma inhibitor, a method for preparing Ulva linza Linnaeus extract for inhibiting Skeletonema costatuma: leaching Ulva linza Linnaeus with sterilized sea water, alcohol, acetone, and aether or chloroform, concentrating the obtained extract by rotary evaporation, putting the concentrated extract into a to-be-processed water body to inhibit red tide alga growth in the water body, where ordinarily, per kg Ulva linza Linnaeus is leached with 30-50 L sea water for 0.5-2 days, the obtained extract is concentrated to 10% of its original bulk, and 2-10 ml concentrate is putted into per L to-be- processed water body. And the invention fully uses Ulva linza Linnaeus in Ulva linza Linnaeus plants, effectively inhibiting growth of harmful marine red tide alga, especially Skeletonema costatuma, and can be widely applied to various water bodies to implement red tide.

Description

technical field [0001] The invention belongs to the field of water treatment, and in particular relates to a method for preparing an inhibitor of Skeletalum costatum. Background technique [0002] Nutrients from land sources and pollutants discharged and leaked from marine operations enter the ocean water body, leading to eutrophication of seawater, which is the main reason for the deterioration of the marine environment. Eutrophication leads to the explosive growth of red tide algae, which will reduce the dissolved oxygen in seawater and bring huge losses to the aquaculture industry. Some algae can also produce algae toxins, which are harmful to aquatic animals and human health. S. costata is a common and main red tide algae in the east coast of China, so S. costata is usually used as a representative of red tide algae. [0003] Existing red tide algae inhibitors are mainly copper ion preparations and synthetic organic herbicides. Copper ions (Cu 2+ ) Killing red tide a...

Claims

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Application Information

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IPC IPC(8): C02F1/00C02F3/00C02F3/32
Inventor 王震宇李锋民安蓁
Owner OCEAN UNIV OF CHINA
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