Novel application of salicornia europaea and root extractive thereof

A technology of salt hornwort and extract, applied in the direction of chemicals, applications, biocides for biological control, etc., can solve the problems of chemical effects related to salt hornwort that have not been seen

Inactive Publication Date: 2010-02-17
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current reports on Salicornia mainly focus on its salt tolerance and salt tolerance mechanism (Ushakova, S.A., N.P.Kovaleva, et al.(2005).Advances in Space Research 36(7):1349-1353.), Ozawa et al. (Ozawa, T., M.Miura,

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  • Novel application of salicornia europaea and root extractive thereof
  • Novel application of salicornia europaea and root extractive thereof
  • Novel application of salicornia europaea and root extractive thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, each extract is to the growth inhibition experiment of three kinds of microalgae

[0034] 1. Test method

[0035] A 100ml Erlenmeyer flask was used in the experiment, containing 40ml of fresh f / 2 culture medium. Three kinds of microalgae and various extract solutions were added respectively. The initial density of algae and the concentration of each extract are shown in Table 1. The microalgae culture solution added with the same volume of f / 2 culture solution was used as the positive control (CK), and the microalgae culture solution added with the same volume of extract solvent was used as the negative control (0mg L -1 ), the petroleum ether phase takes petroleum ether as a contrast, the water phase takes f / 2 culture fluid as a contrast, and the other three phases take acetone as a contrast, and the final concentration of each solvent in the culture fluid is 0.1%. The results show that under this concentration, petroleum Ether and acetone had no effect...

Embodiment 2

[0059] Embodiment 2, culture water filtrate is to the growth inhibition experiment of two kinds of microalgae of Skeletalum costa and Prorocentrum donghaiense

[0060] In order to verify whether the effective grams of biomass can be secreted into the environment, we took S. costatum and P. donghaiense as the experimental objects, and carried out biological identification using the cultured and filtered water of Salicornia.

[0061] experiment method:

[0062] The inoculum biomass will be 10, 30, 60g FW L -1 Salicornia salicornia was cultured in f / 2 culture solution for 30 days, then the salicornia salicornia was removed, and then the culture solution was filtered through high-pressure sterilized filter membranes (Whatman GF / F, 0.65 μm and cellulose acetate filter membrane, 0.22 μm ) and re-enriched with f / 2 nutrient solution, adjusting pH and salinity to 8.0±0.02 and 30 psu. S. costatum and P. donghaiense were immediately inoculated into the filtrate of salicornia culture wa...

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Abstract

The invention discloses novel application of salicornia europaea and a root extractive thereof. The salicornia europae (Salicornia europaea L.) and the root extractive thereof provided by the invention are used for inhibiting the algae growth causing red tide and inhibiting the red tide. The algae causing the red tide is selected from at least one of skeletonema costatum, prorocentrum donghaiense and prorocentrum minimum. The method for preparing the root extractive of the salicornia europae comprises the following steps: using an organic solvent as an extractant, and extracting from the root of the salicornia europaea, wherein the organic solvent is selected from any one of petroleum ether, chloroform, methanol and normal butanol. The result of the test shows that four different root extractives of the salicornia europaea can strongly inhibit the growth and the photosynthesis of three kinds of algae; for different algae, the allelopathy strength is different; for the skeletonema costatum, the allelopathy of the petroleum ether phase extractive is the strongest, and IC50 is 1.633 mg/L; for the prorocentrum donghaiense, the function of the chloroform phase extractive is the strongest, and IC50 is 13. 799 mg/L; and for the prorocentrum minimum, the allelopathy is improved along with the increasing of the polarity of the extractive, the function of the methanol phase is the strongest, and IC50 is 1.138 mg/L.

Description

technical field [0001] The invention relates to a new application of salt hornwort and its root system extract. Background technique [0002] Red tide is one of the marine environmental problems that the international community is concerned about and urgently needs to be resolved. Since the 1990s, the occurrence of red tides in my country has shown the characteristics of "large-scale, high-frequency, multiple types, and fast-paced", which poses a great threat to the ecological environment of coastal waters and the sustainable development of aquaculture, and at the same time endangers tourism. industrial development and human health. Adopting effective red tide control measures is one of the important contents of red tide research. Scholars at home and abroad have proposed including using loess, clay, copper sulfate and some biological agents like bacteria and viruses to control red tide. While these approaches may be effective in the short term, they can cause varying degr...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P13/00
Inventor 李银心黄凌风姜丹
Owner INST OF BOTANY CHINESE ACAD OF SCI
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