Skeletonema costatum culture medium formula and preparation method thereof, and culture method
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A technology of medium formula and culture method, which is applied in the field of culture medium formula and plastic film suspension hanging bags of Skeletons costa, which can solve the problems of long doubling time, slow growth of algae cells, easy pollution, etc.
Inactive Publication Date: 2014-03-26
LINYI UNIVERSITY
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[0004] At present, the main problems in the large-scale culture of Skeletodes costata are the slow growth of algae cells, long doubling time, and easy pollution.
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[0011] Culture medium preparation method:
[0012] 1) According to the order and dosage of nutrient salts in the formula, add nutrient salts in sequence;
[0013] 2) Due to iron citrate (FeC 6 h 5 o 7 ·5H 2 O) It is difficult to dissolve, you can add a small amount of tap water and heat it slightly to 80-90°C on the stove, and keep stirring until it is completely melted;
[0014] 3) 920 plant growth stimulant (namely gibberellin) is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved with alcohol first (generally dissolve with 50 ml of alcohol or 60-degree liquor for more than 1 hour per gram), and then Dilute with water to desired concentration.
[0015] 4) After adding all the nutrient salts, inflate and stir for 30 minutes before inoculating.
[0016] 5) This medium formula is applicable to both the expansion stage and the large-scale cultivation stage of Skeletalum costanum.
[0017] Culture method of Skeletodera mesoscarci...
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Abstract
The invention provides a Skeletonema costatum culture medium formula. A specific technical scheme is as follows: a Skeletonema costatum culture medium formula is as follows: 0.4g of KNO3, 4mg of KH2PO4, 0.5g of NaHCO3, 5 IU of a 920 growth hormone (namely gibberellin), 1 ml of an f / 2 trace element solution, 1 ml of an f / 2 vitamin solution and 1000 ml of seawater. The f / 2 trace element solution comprises 23 mg of ZnSO4.4H2O, 178 mg of MnCl2.4H2O, 10 mg of CuSO4.5H2O, 3.9 g of FeC6H5O7.5H2O, 7.3 mg of Na2MoO4.2H2O, 12 mg of CoCl2.6H2O, 4.35 g of Na2EDTA, and 1000 ml of pure water; and the f / 2 vitamin solution comprises 0.5 mg of vitamin B12, 100 mg of vitamin B1, 0.5 mg of vitamin H and 1000 ml of pure water. The optimized medium and a plastic film suspension sling method for culturing Skeletonema costatum have advantages of fast growth, short production cycle, high yield, high content of highly unsaturated fatty acid, low cost, easy operation, no pollution, easy control, flexibility, safety and reliability.
Description
technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a culture medium formula of Sclerotina costa and a method for cultivating it with a plastic film suspension hanging bag. Background technique [0002] Skeletonema costatum (Skeletonema costatum) belongs to Diatomphyta, Central Class, Cyclophylles, Skeletoneceae, and Skeletonema genus. The cells are lens-shaped or cylindrical, with a diameter of 6-7 microns, and form a chain group with the corresponding spines of adjacent cells. It can grow and reproduce at ~34°C. It has the characteristics of rich nutrition and easy cultivation. It is an ideal opening bait for prawns, river crabs, swimming crabs, blue crab larvae, and oyster larvae in the middle and late stages. [0003] Inorganic carbon in seawater exists in the form of HCO 3 - Mainly (90%), while free CO 2 Less than 1%. In the past, it was believed that free CO in water 2 It is the only form of exo...
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