Detection probe of Skeletonema costatum

Abstract

A detecting probe for mid-rib-bar seaweed is disclosed. A group of probes, whose core is SI enzyme protecting and analyzing probe, is synthesized for the particular rRNA region of mid-rib-bar seaweed in order to qualitatively and quantitatively detect the rRNA of said seaweed. Said tetecting probe includes Si enzyme protecting and analyzing probe, capture probe and signal probe or binding probe.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for the qualitative and quantitative detection of Skeletalum costatum. It belongs to the field of nucleic acid detection. Background technique [0002] Skeletonema costatum is a kind of eurythermal and euryhaline phytoplankton that widely exists in the world, and it is also one of the most common dominant species of diatoms in my country's coastal waters. This species is a good bait for marine secondary producers, and it is also a common red tide organism in my country's coastal waters and a good pollution indicator. In economical seaweed farming areas, it often competes with kelp and laver for nutrients. Cause economic algae to change color, even rot and lose edible and commercial value; it is also reported that the bloom of Sclerostena mesoscarcinus as the dominant species can cause damage to the gills of Atlantic salmon and eventually die...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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