Method for preparing phenolic acid and alkaloid by separation and purification from ulva pertusa kjellm and application thereof

A technology for separation, purification, and alkaloids, applied in the fields of botanical equipment and methods, chemicals for biological control, applications, etc. Effect

Active Publication Date: 2014-04-02
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Ulva kongfu is mainly used in aquaculture feed and ecol...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, a method for separating and purifying phenolic acids and alkaloids from Ulva pore, the steps are as follows:

[0015] (1) Add anhydrous methanol to the dry powder of Ulva ulvae, extract under ultrasonic waves at room temperature for 2 hours, filter and evaporate to dryness under reduced pressure to prepare the evaporated product; add the evaporated product to distilled water, shake fully, stand overnight at 4°C, centrifuge, Filter to remove the precipitate to obtain the supernatant;

[0016] (2) Adjust the pH of the supernatant to 11, add ethyl acetate for extraction; the obtained upper layer was evaporated to dryness under reduced pressure to obtain component A, the obtained lower layer was adjusted to pH 7, extracted with ethyl acetate, and the obtained upper layer was evaporated to dryness under reduced pressure, Prepare component B, then adjust the pH of the obtained lower layer to 2, extract with ethyl acetate, and evaporate the obtained upper layer a...

Embodiment 2

[0019] Example 2, a method experiment for the separation and purification of phenolic acids and alkaloids from Ulva pore:

[0020] The dry Ulva kongii was crushed into 0.3mm powder, added with anhydrous methanol, and extracted under ultrasonic wave at room temperature for 2 hours. After repeated extraction for 3-4 times, combine the extracts, centrifuge, filter and evaporate to dryness under reduced pressure to prepare the evaporated product; add an appropriate amount of distilled water to the evaporated product, shake fully, let stand overnight at 4°C, centrifuge, filter, and remove the precipitate , keep the supernatant. Use 2-5mol / L sodium hydroxide to adjust the pH of the supernatant to 11, add ethyl acetate to extract 3-4 times. The upper layer was evaporated to dryness under reduced pressure to obtain component A. Adjust the pH of the lower layer to 7, extract 3-4 times with ethyl acetate, and evaporate the upper layer to dryness under reduced pressure to prepare compo...

Embodiment 3

[0022] Example 3, a method experiment for the separation and purification of phenolic acids and alkaloids from Ulva pore:

[0023] Weigh 400g of dry Ulva pore powder, dissolve it in 2000mL of anhydrous methanol, and ultrasonically extract it at room temperature for 2h. After extracting 4 times, the extracts were combined, centrifuged, filtered and evaporated to dryness under reduced pressure to prepare 42.6 g of evaporated matter. Add 40 mL of distilled water to the evaporated product, shake it well, let it stand overnight at 4°C, centrifuge and filter to remove the precipitate, and keep the supernatant. The pH of the supernatant was adjusted to 11 with 2 mol / L sodium hydroxide, and ethyl acetate was added to extract 4 times. The upper layer was evaporated to dryness under reduced pressure at 40°C to obtain 2.219 g of dark green evaporated matter (component A). The pH of the lower layer was adjusted to 7, extracted 4 times with ethyl acetate, and the upper layer was evaporat...

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PUM

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Abstract

The invention provides a method for preparing phenolic acid and alkaloid by separation and purification from ulva pertusa kjellm. The method comprises the following steps: adding ulva pertusa kjellm dry powder into absolute methanol to prepare a steamed object, and adding distilled water to prepare supernate; regulating pH of the supernate, and adding ethyl acetate to extract for multiple times to obtain components A, B, C and D; spotting methanol solution to silica gel GF254, combining and collecting strips at same Rf positions, dissolving in acetone, preparing eleven components by centrifuge and concentration in reduced pressure, and spotting four of the components to the silica gel GF254 by a streaking method once again for purifying preparation to obtain six components, wherein thirteen components are phenolic acid and alkaloid, and can be used for inhibiting growth of karenia mikimotoi or skeletonema costatum. The method is strong in operability, the prepared phenolic acid and alkaloid are high in purity, and the breakthrough of phenolic acid and alkaloid prepared by extracting ulva pertusa kjellm can be realized. The phenolic acid and alkaloid prepared by the method have algal inhibition activity, and have practical application values.

Description

technical field [0001] The invention belongs to the field of marine biochemical engineering, in particular to a method for separating and purifying phenolic acids and alkaloids from Ulva pore. The present invention also relates to the use of the phenolic acid and alkaloid prepared by the method. Background technique [0002] At present, red tides are common in coasts all over the world, which poses a great threat to the ecological environment of offshore waters, human life and the sustainable development of coastal aquaculture. While the occurrence frequency, scale and harm of red tides are becoming more and more serious, green water such as Ulva sp. and Enteromorpha sp. Seaweed, mainly represented by algae, also began to flood, forming algae blooms, causing significant losses to coastal fisheries, aquaculture and tourism. How to use these green tide seaweed resources scientifically and effectively to reduce the economic loss caused by green tide has become one of the prob...

Claims

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Application Information

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IPC IPC(8): A61K36/05A01N65/03A01P21/00
Inventor 孙颖颖阎斌伦王辉
Owner HUAIHAI INST OF TECH
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