Method for detecting growth rate of skeletonema costatum

Abstract

The present invention discloses a method to test the growth rate of the skeletonema costatum, which includes the procedures as follows: a. the extraction of RNA of skeletonema costatum, b. separation and cloning of partial sequences of the PCNA gene of the skeletonema costatum, c. cloning of partial sequences of Cytb gene of skeletonema costatum, d. a standard real-time quantitative PCR curve used to test genes of PCNA and Cytb of skeletonema costatum is developed, based on the gene sequences of both PCNA and Cytb, e. the growth rate of the skeletonema costatum is tested based on the expression of quantity (REQ) of gene PCNA of skeletonema costatum, with PCNA as the target gene and the Cytb as the housekeeping gene. The present invention provides a technical method for accurate estimation on the growth rate on the spot of the skeletonema costatum, which can be used to estimate the productivity of the floating algae, study the variance of the alga species, construct the zoological dynamic model and even forecast the red tide.

Description

technical field [0001] The invention relates to a method for detecting planktonic diatoms, in particular to a method for detecting the growth rate of Skeletalum costatum by real-time fluorescent quantitative PCR. Background technique [0002] Scutellaria mesocostalis is a typical representative of wide temperature and wide salt. It is widely distributed, from the arctic to the equator, from high salt water in open seas to coastal low salt water masses, and even in brackish water, but it is most common in the coast. Sclerostena mesospinatus mostly forms dominant species in late summer and early autumn, and causes red tides. When red tides occur, the cell density is 4.3×10 3 ~7.2×10 5 mL -1 (Wang et al., 1991). The density of Scutellaria costatum in the critical state of red tide in the Yangtze Estuary is 7×106 m-3, accounting for about 96% of the total phytoplankton (Hong et al., 1992). In my country's Pearl River Estuary in the South China Sea, the Jiulong River Estuary ...

AI Technical Summary

Problems solved by technology

[0015] The technical problem to be solved by the present invention is to provide a technical method for accurately estimating the on-site growth rate of Scutellaria costarum, which is used for estimating the productivity of planktonic algae, studying the change of planktonic algae populations, constructing ecological dynamic models and even predicting red tides, overcoming existing The defect that basic physiological and ecological parameters such as the on-site growth rate of a single species of planktonic algae cannot be measured in the technology

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