Detection probe for phycobiont

Abstract

A detecting probe for phycobiont is disclosed. A group of probes, whose core is S1 enzyme protecting and analyzing probe, is synthesized for the particular rRNA region of phycobiont in order to quaitatively and quantitatively detect the rRNA of phycobiont. Said detecting probe includes S1 enzye protecting and analyzing probe, capture probe and signal probe or binding probe.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for the qualitative and quantitative detection of a symbiotic algae. It belongs to the field of nucleic acid detection. Background technique [0002] Symbiotic algae (Symbiodinium sp.) is a symbiotic dinoflagellate often found in marine invertebrates such as clams, sea anemones, jellyfish, foraminifers and reef-building corals, etc., which live symbiotically within invertebrate-microalgae The environment plays a key role. As a global environmental problem, the degradation of coral reefs has attracted increasing attention, and symbiotic algae play a vital role in obtaining sufficient nutrients for coral polyps to form a calcareous skeleton to construct coral reefs. Therefore, timely, accurate and rapid detection of the population dynamics of symbiotic algae in the marine environment, and keeping abreast of their quantity changes in the ocea...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

Images