Detecting probe for Pseudo-nitzchia pungens

Abstract

A detecting probe for the oxyacanthous pseudodiamond seaweed includes mutually correlative three oligonucleotide probes including S1 enzyme protecting and analyzing probe. It can be used to protect and analyze S1 enzyme and detect it by sandwich hybridization.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for qualitative and quantitative detection of Pseudomonas spinosa. It belongs to the field of nucleic acid detection. Background technique [0002] Pseudo-nitzschia pungens is a wide-temperate nearshore species, which is distributed in the coast of my country and is one of the main red tide organisms. In the East my country Sea of ​​China, there are many records of forming toxic red tides. Marine fisheries and mariculture have had some impact. Therefore, it is of great significance to timely, accurately and quickly detect the population dynamics of Pseudomonas spinosa in the marine environment and keep abreast of its quantity changes in the ocean. The traditional identification method of directly observing and counting with an optical microscope is cumbersome, time-consuming, and laborious, and requires the participation of experienced taxo...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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