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Detecting probe of round hailian seaweed

A technology for detecting probes and thalassina, which is applied in the field of oligonucleotide probes and specific probes, can solve the problems of large fluctuations in detection results, small number of captured probes, and difficult operation, so as to facilitate the search and realization, good probe applicability and high stability

Inactive Publication Date: 2005-06-08
OCEAN UNIV OF CHINA
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Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

Method used

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  • Detecting probe of round hailian seaweed
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Embodiment

[0030] Example: Qualitative and quantitative detection of Thalassiosira rubrum by using S1 enzyme protection analysis for rRNA and sandwich hybridization technology.

[0031] In this example, the S1 enzyme protection analysis technique and the sandwich hybridization technique are combined to realize the qualitative and quantitative detection of the marine red tide organism Thalassiosira rotula, and the steps are as follows:

[0032] 1.1 The search for the specific region of Thalassiosira torum rRNA and the design and synthesis of the S1 enzyme protection analysis probe

[0033] By determining the 18S rRNA sequence of the ribosomal small subunit of Thalassiosira cyclamen and performing multiple sequence comparisons with the rRNA sequences of other algae, it was found that the 1670-1740 region of the small subunit rRNA was different from other algae, and the sequence of this region was determined to be a characteristic sequence ; Design and synthesize the S1 enzyme protection an...

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Abstract

A testing probe for a coscinodiscus, concretely aiming at the rRNA special area of coscinodiscus, compose a group of testing probe having s1 enzyme protecting analysis probe as the core, combining s1 enzyme protecting analysis with sandwich hybridization technology to realize the quantitative and qualitative testing for rRNA of coscinodiscus. The probe includes three correlative oligonucleotide probe :S1 enzyme protecting analysis probe, catching probe, signal probe or connection probe.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for qualitative and quantitative detection of Thalassiosira torticola. It belongs to the field of nucleic acid detection. Background technique [0002] Thalassiosira rotula is a planktonic species in the temperate zone. It is distributed in the Yellow Sea, East China Sea and South China Sea. It is one of the main red tide organisms in the East China Sea. There are many records of large-scale red tides. Fisheries and mariculture have had some impact. Therefore, it is of great significance to detect the population dynamics of Thalassiosira in the marine environment in a timely, accurate and rapid manner, and to keep abreast of its quantity changes in the ocean. The traditional identification method of directly observing and counting with an optical microscope is cumbersome, time-consuming, and laborious, and requires the participation of exp...

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Application Information

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IPC IPC(8): C12Q1/68G01N33/50
Inventor 于志刚李荣秀蔡青松米铁柱甄毓
Owner OCEAN UNIV OF CHINA
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