Detection probe of phaeocystis

Abstract

A detecting probe of brown colpomenia for protecting and analyzing the S1 enzyme of said seaweed and detecting it by sandwich hybridization has mutually correlative three oligonucleotide probes including the S1 enzyme protecting and analyzing probe.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for algae detection, in particular to specific probes for qualitative and quantitative detection of Phaeocystis. It belongs to the field of nucleic acid detection. Background technique [0002] Phaeocystis sp. is a kind of poisonous algae, which can produce toxins with hemolytic effect. It belongs to Chrysophyta and Dinoflagellates. It has caused red tides many times in the southern waters of my country. There is a layer of soft gelatinous algae on the periphery of the algal spherical group, and the algae contain polysaccharides. When a large number of red tides are formed, the algae containing gelatin and sugar will be tightly attached to the gills of the fish, affecting the breathing and feeding of the fish. Cause fish to suffocate and die due to lack of oxygen; secondly, the huge biomass of the algae (especially at dawn and evening) can cause water hypoxia and cause disasters. Coupled with...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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