Preserving fluid for preserving bacterial DNA in excrement sample at normal temperature

Abstract

The invention provides a preserving fluid for preserving bacterial DNA in an excrement sample at normal temperature. The preserving fluid comprises sodium chloride, absolute ethyl alcohol, a biological bacteriostatic agent, disodium EDTA, and deionized water. The preserving fluid comprises the following components: 0.5-2 mol / L of the sodium chloride, 20-60% (v / v) of the absolute ethyl alcohol, 0.01-0.2% (v / v) of the biological bacteriostatic agent, 10-100 mmol / L of the disodium EDTA, and the balance of the deionized water. The pH value of the preserving fluid is 7-8, and the biological bacteriostatic agent is Proclin 300. According to the preserving fluid for preserving the bacterial DNA in the excrement sample at normal temperature, the situation that the DNA of microorganisms in the excrement sample is not degraded in a normal-temperature environment can be guaranteed, the number, the composition and the abundance of the microorganisms in the excrement sample are kept stable throughgood storage performance, and the accuracy of data analysis after intestinal flora sequencing is guaranteed; and the normal-temperature preservation and the transportation of the excrement sample areachieved, the preserving fluid is suitable for long-distance transportation, and compared with an existing preserving fluid on the market, the preserving fluid has the advantages of being good in preservation effect, easy to operate, good in applicability and free of toxicity and harm.

Description

technical field [0001] The invention relates to the technical field of DNA preservation solution, in particular to a preservation solution for preserving bacterial DNA in feces samples at normal temperature. Background technique [0002] Low-temperature cryopreservation is the most common method to preserve microorganisms in fecal samples, but cryopreservation usually requires large-scale refrigeration equipment, which is not universal and complicated to operate. Therefore, it is necessary to develop a DNA preservation solution suitable for microbial preservation. [0003] At present, the DNA preservation solutions on the market usually contain toxic components such as guanidine isothiocyanate, or the single component makes the microorganisms in the sample easy to contaminate, the composition and quantity of the microorganisms change, and cannot truly reflect the biological information of the sample. . Contents of the invention [0004] In order to solve the above problem...

AI Technical Summary

Problems solved by technology

[0002] Low-temperature cryopreservation is the most common method to preserve microorganisms in fecal samples, but low-temperature preservation usually requires large-scale refrigeration equipment, which is not universal and complicated to operate. Therefore, it is necessary to develop a DNA preservation solution suitable for microbial preservation

[0003] At present, the DNA preservation solutions on the market usually contain toxic components such as guanidine isothiocyanate, or the single component makes the microorganisms in the sample easy to contaminate, the composition and quantity of microorganisms change, and cannot truly reflect the biological information of the sample

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