Detection probe of phaeocystis

Abstract

A detecting probe of brown colpomenia for protecting and analyzing the S1 enzyme of said seaweed and detecting it by sandwich hybridization has mutually correlative three oligonucleotide probes including the S1 enzyme protecting and analyzing probe.

Description

Technical field [0001] The invention relates to a set of oligonucleotide probes for the detection of algae, in particular to specific probes for the qualitative and quantitative detection of Phaeocystis. It belongs to the field of nucleic acid detection. Background technique [0002] Phaeocystis sp. is a toxic algae that can produce toxins with hemolytic effect. It belongs to the Chrysophyta and Phaeocystis sp. It has caused red tides in the southern waters of my country many times. The algae spherical colony has a layer of soft gelatinous covering and the algae contains polysaccharides. When the algae multiply to form a red tide, the algae containing gelatin and sugars will cling to the gills, affecting the respiration and feeding of the fish. It causes fish to suffocate and die due to lack of oxygen; secondly, the huge biomass of the algae (especially at dawn and evening) can cause water shortages and cause disasters. In addition, the death and decay of algae and algae cells wi...

AI Technical Summary

Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

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