Detection probe for ocean Prorocentrum
A technology for detecting probes and Prorocentrum, applied in the field of oligonucleotide probes, can solve the problems of small number of capture probes, large fluctuation of detection results, and difficult operation
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[0029] Example: Qualitative and quantitative detection of marine Prorocentrum by using S1 enzyme protection analysis for rRNA and sandwich hybridization technology
[0030] In this embodiment, the S1 enzyme protection analysis technique and the sandwich hybridization technique are combined to realize the qualitative and quantitative detection of marine red tide organism Prorocentrum micans (Prorocentrum micans), and the steps are as follows:
[0031]1.1 The search for the specific region of marine Prorocentrum rRNA and the design and synthesis of the S1 enzyme protection analysis probe. The 430-520 region of the large subunit rRNA is different from other algae, and the sequence of this region is determined to be a characteristic sequence; according to the specific
[0032] Sequence design and synthesis of S1 enzyme protection analysis probe MIC_S1: 5'-ACCAGGCACATTCACCCACCCAGGGGTAGGCTACCATGTCCCTGGAGTTTTCCTCGAT (SEQ ID NO.1).
[0033] Design and synthesis of additional probes r...
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