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Detection probe for Scrippsiella trochoidea

A technology for detecting probes and Steineria, which is applied in the determination/inspection of microorganisms, biological testing, material inspection products, etc., and can solve the problems of large fluctuations in test results, difficult operation, and small number of capture probes, and achieve Easy to find and realize, good probe applicability and high stability

Inactive Publication Date: 2005-06-29
OCEAN UNIV OF CHINA
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Problems solved by technology

Its main disadvantages are: first, because the length of the capture probe is only 10-30 bp, the number of capture probes that can effectively distinguish close relatives of microalgae is very small; second, it is necessary to prevent RNA degradation during the entire detection process, However, various steps such as washing and antibody reaction can easily cause RNA degradation, so the operation is difficult and the test results fluctuate greatly

Method used

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  • Detection probe for Scrippsiella trochoidea
  • Detection probe for Scrippsiella trochoidea

Examples

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Embodiment

[0030] Example: Qualitative and quantitative detection of Steineria coniculae by using the S1 enzyme protection analysis for rRNA and the sandwich hybridization technique.

[0031] In this example, the S1 enzyme protection analysis technique and the sandwich hybridization technique are combined to realize the qualitative and quantitative detection of the marine red tide organism Scrippsiella trochoidea, and the steps are as follows:

[0032] 1.1 The search for the specific region of Steineria cone rRNA and the design and synthesis of the S1 enzyme protection analysis probe

[0033] By determining the ribosomal small subunit 18S rRNA sequence of Steineria conicerii and performing multiple sequence comparisons with the rRNA sequences of other algae, it was found that the 230-300 region of the small subunit rRNA was different from other algae, and the sequence of this region was determined to be a characteristic sequence ; Design and synthesize the S1 enzyme protection analysis p...

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Abstract

This invention discloses a pyramidal Si alga test probe, which belongs to nucleic acid test field and comprises the following steps: to integrate a group of probes centered as Si enzyme protective probe in the specific area of rRNA; to combine the Si enzyme protective analysis and clamping crossbreed technique to realize the ration measurement of the pyramidal Si alga. The said probes comprise three connected oligonucleotide probes, Si enzyme protective probe, catching probe, and signal probes or connection probe.

Description

technical field [0001] The invention relates to a group of oligonucleotide probes for detection of algae, in particular to specific probes for qualitative and quantitative detection of Stirrella cones. It belongs to the field of nucleic acid detection. Background technique [0002] Scrippsiella trochoidea is a flagellar algae that is widely distributed and common in coastal waters around the world. The record of red tide has caused a certain impact on marine fishery and mariculture. Therefore, it is of great significance to timely, accurately and quickly detect the population dynamics of Steinerii in the marine environment and keep abreast of its quantity changes in the ocean. The traditional identification method of directly observing and counting with an optical microscope is cumbersome, time-consuming, and laborious, and requires the participation of experienced taxonomy experts. When the amount of samples is large, the biodiversity is rich, and the monitoring range is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50
Inventor 于志刚李荣秀蔡青松米铁柱甄毓
Owner OCEAN UNIV OF CHINA
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