Schizochytrium limacinum strain detection probe

Abstract

A Schizochytrium limacinum strain detection probe group belongs to the field of nucleic acid detection. The invention includes three strips of interrelated oligonucleotide probes: an analysis probe for specific binding of rRNA of the Schizochytrium limacinum (S.limacinum), a capture probe for binding on a vector and specific binding with the analysis probe, and a signal probe for specific binding with the analysis probe. The analysis probe has a nucleotide sequence shown as in a SEQ ID NO:1; the capture probe has a sequence shown as in a SEQ ID NO:2 and has biotin labeling; and a signal probe has a sequence as shown in a SEQ ID NO:3 and has an isothiocyanic acid (FITC) label at the 5' terminal. The detection probe group is in linear correlation (R2=0.9942) with detection signal strength when cell concentration of S.limacinum is within 0.29*10<3>-2.1*10<3> in per milliliter of nutrient solution, has the advantages of good specificity, high sensitivity, high stability, qualitative and quantitative research, and is used for quality inspection during breeding and fermentation production process of the S.limacinum strain.

Description

technical field [0001] The present invention relates to a group of Schizochytrium ( Schizochytrium limacinum ) The oligonucleotide probe for strain detection, specifically the specific detection probe for qualitative and quantitative detection of Schizochytrium, belongs to the field of nucleic acid detection. Background technique [0002] In recent years, docosahexaenoic acid (22:6, n-3, docosahexaenoic acid, DHA) has been favored for its important physiological functions in humans and animals. Traditionally, DHA is obtained from deep-sea fish oil, which has problems such as unstable output, low yield, high cost, and other ω-6PUFAs. With the increasingly tense fishery resources, it has been unable to meet the growing market demand. . Therefore, the development of new resources of DHA has become a new research focus. [0003] Schizochytrium is a marine fungus with high DHA content discovered by foreign scientists in the 1990s, belonging to Labyrinthulomycota, Labyrinthulom...

AI Technical Summary

Problems solved by technology

These methods have great limitations: First, the specificity is poor. Microscopic observation can only identify bacterial species with large individual differences from the production strains, and plate separation can only identify colonies that can grow on the plate and are greatly different from the production strains. The second is that it takes a long time, such as plate culture, it generally takes at least 2 days, and the industrial fermentation time is about 5 days, so it cannot reflect the situation in the fermentation broth in time; the third is that it cannot be qualitative for high-yield strains Quantitative detection and microscopic observation can only achieve quantitative analysis of strains with similar appearance, while the plate separation method has a large error in quantification

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